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Preparation and Optimization of Chitosan/pDNA Nanoparticles Using Electrospray

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Chitosan (CS), as a natural polymer, has received a great deal of attention as a carrier for delivery of biomaterials. Biomaterials can be incorporated directly into chitosan nanoparticles using the electrospray method; a cost-effective method with mild condition, suitable for sensitive therapeutic agents. In the present work, the authors prepared chitosan/pDNA nanoparticles by electrospray, and evaluated the influence of process/formulation parameters such as chitosan molecular weight, N/P ratio (moles of CS amino groups to phosphate groups of DNAs) and applied voltage on the size of chitosan/pDNA nanoparticles. Furthermore, these nanoparticles were optimized by artificial neural networks for prediction of particle size. Increase in applied voltage and N/P ratio with a decrease in molecular weight led to the formation of smaller particles. Eventually, chitosan/pDNA nanoparticles with minimum size of 152 (nm) were obtained with molecular weight, N/P ratio and applied voltage values of 7 (kDa), 9, and 15 (kV), respectively.

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Acknowledgements

The authors are grateful to the Tehran University of Medical Sciences and Health Services for providing facilities and financial support through Grant No. 93-01-87-25230.

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Authors and Affiliations

  1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Morteza Abyadeh, Mahdi Aghajani & Amir Amani

  2. Department of Medical Nanotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Arezoo Gohari Mahmoudabad

  3. Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Amir Amani

Authors
  1. Morteza Abyadeh
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  2. Mahdi Aghajani
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  3. Arezoo Gohari Mahmoudabad
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  4. Amir Amani
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Correspondence to Amir Amani.

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The authors declare that they have no conflict of interest to publish this manuscript.

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The authors have not conducted any studies with human participants or animals, for this article.

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Significance Statement

Electrospray is an efficient method for direct production of polymeric nanoparticles. However, the effect of various processing parameters on the formation of chitosan/pDNA has not been studied in depth. In the present study, the authors have investigated the effect of three (3) important variables on the size of chitosan/pDNA nanoparticles prepared, using electrospray.

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Abyadeh, M., Aghajani, M., Gohari Mahmoudabad, A. et al. Preparation and Optimization of Chitosan/pDNA Nanoparticles Using Electrospray. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 931–937 (2019). https://doi.org/10.1007/s40011-018-1009-6

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  • DOI: https://doi.org/10.1007/s40011-018-1009-6

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