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Plant Growth-Promoting Potential of Endophytic Bacteria Isolated from Costus speciosus in Tropical Deciduous Forest of Eastern Himalaya

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Endophytic bacteria play an important role in plant growth. In the present investigation, the authors examined the endophytic bacteria associated with tissues (root, stem, leaf) of Costus speciosus (J. Koenig) Sm. collected from the tropical deciduous forest of Meghalaya along with their plant growth promotion traits. Scanning electron microscopy and epifluorescence microscopic studies revealed the presence of bacterial cell internal to the tissues of the plant species. A total of 122 endophytic bacteria were isolated where the extent of colonization varied in plant parts. The molecular characterization of endophytic bacteria showed that bacterial communities associated with plant species are composed of 11 genera comprising actinobacteria, firmicutes and alpha-proteobacteria. Roots unveil the highest diversity and richness of endophytic bacteria, followed by leaf and stem. On evaluating the isolates for plant growth promoting properties, it was found that eight (8) isolates can produce both; indole acetic acid and siderophore, five (5) have phosphate solubilizing activity and six (6) have ACC deaminase activity. The results confirmed that Costus speciosus in tropical deciduous forest of Meghalaya encompasses diverse endophytic bacterial communities that could be potentially useful in promoting plant growth.

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Acknowledgements

The authors are highly thankful to the State Biotech Hub (SBTHub), North-Eastern Hill University, Shillong, funded by DBT, Government of India, New Delhi for providing the required instrumentation facilities. They are also thankful to the Department of Science & Technology, Government of India, for providing financial assistance in the form of fellowship (INSPIRE Fellow). They also thank Dr. Kaushik Bhattacharjee, N.E.H.U., Shillong for his valuable suggestions.

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  1. Microbial Ecology Laboratory, Centre for Advanced Studies in Botany, Department of Botany, North-Eastern Hill University, Shillong, 793022, India

    Dina Barman & Mamtaj S. Dkhar

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  1. Dina Barman
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  2. Mamtaj S. Dkhar
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Correspondence to Dina Barman.

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Significance statement Tissue specific diversity of endophytic bacteria from Costus speciosus in tropical deciduous forest of Eastern Himalaya with plant growth promoting activity.

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Barman, D., Dkhar, M.S. Plant Growth-Promoting Potential of Endophytic Bacteria Isolated from Costus speciosus in Tropical Deciduous Forest of Eastern Himalaya. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 841–852 (2019). https://doi.org/10.1007/s40011-018-0998-5

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  • DOI: https://doi.org/10.1007/s40011-018-0998-5

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