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Substitutions accrued on Foot-and-mouth disease virus capsid during propagation in cell culture

  • Laxmi N. Sarangi
  • Jajati K. Mohapatra
  • Saravanan Subramaniam
  • Biswajit Das
  • Aniket Sanyal
  • Bramhadev Pattnaik
Research Article
  • 27 Downloads

Abstract

Three lineages of serotype O of foot-and-mouth disease virus were passaged serially in BHK-21 cell culture without application of any immune pressure, to study the frequency, nature and location of the substitutions accruing on the virus capsid. The viruses showed unusual stability as only 12 substitutions were observed in 13 different regimens and the majority of the substitutions reverted back to the parental genotype very soon after their appearance. Of the 12 substitutions, a maximum of 8 were found in the VP1 region. Some substitutions (81, 147, 152, 203 and 210 in VP1 and 50 in VP3) were observed at the established antigenic sites suggesting that antigenic diversification can occur in the absence of immune selection. The viruses after serial cytolytic infection of BHK-21 cells, demonstrated an ability to infect the integrin-deficient CHO-K1 cell line suggesting an expansion in their receptor usage potential. Even after 25–50 passages in BHK-21 cell system no histidine to arginine switch was observed at the 56th residue of VP3. Amino acid sequence analysis of 141 Indian field isolates for the residues involved in heparin binding sites suggest the importance of net positive charge in the HS-binding pocket or elsewhere on the capsid for interaction with the alternative receptors and cell culture adaptation rather than acquisition of positive charge at any particular position for all serotype O strains.

Keywords

Foot-and-mouth disease virus Serotype O virus Serial cytolytic infection Antigenic sites Heparin binding sites 

Notes

Acknowledgements

The authors are thankful to Indian Council of Agricultural Research for providing necessary facilities to carry out this work. The first author is thankful to ICAR for providing senior research fellowship during his doctoral degree.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • Laxmi N. Sarangi
    • 1
    • 2
  • Jajati K. Mohapatra
    • 1
  • Saravanan Subramaniam
    • 1
  • Biswajit Das
    • 1
  • Aniket Sanyal
    • 1
  • Bramhadev Pattnaik
    • 1
  1. 1.ICAR- Directorate of Foot-and-Mouth DiseaseMukteswar-Kumaon, NainitalIndia
  2. 2.National Dairy Development Board Research and Development LaboratoryHyderabadIndia

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