Recombinant Partial Conglutinin of Buffalo and Nilgai In Vitro Can Mimic the Functions of Native Conglutinin In Vivo

  • D. Ramesh
  • S. Chandra Mohan
  • Mohini Saini
  • Sasmita Barik
  • M. Shynu
  • Asit Das
  • Anil Kumar Sharma
  • Vinod Kumar Chaturvedi
  • Praveen Kumar Gupta
Research Article
  • 19 Downloads

Abstract

Conglutinin, a high molecular weight protein detected in bovine serum, belongs to the family of innate immunity markers called collectins. It plays a key role as antimicrobial agent against all kinds of micro-organisms by binding to sugar moieties present on them. In the present study, partial recombinant conglutinin consisting neck and carbohydrate recognition domain of buffalo and nilgai was expressed in prokaryotic system and found to be of 27 kDa through SDS-PAGE and western blotting using antiserum raised against buffalo conglutinin. In the presence of calcium, protein showed high binding activity towards mannan coated to microtitre plate but in contrast, binding activity towards lipopolysaccharide was calcium independent, which needs to be explored further. The partial protein was unable to inhibit haemagglutination by Newcastle disease virus; this could be attributed to lack of complete protein, as complete protein forms tetramer which can cross link the virus. The protein was also found to reduce bovine herpes virus-1 titre propagated in MDBK cells. This paves path to check the effect of complete conglutinin protein on different enveloped viruses, which will provide insight into protein’s importance in innate immunity. Since the concentration of serum conglutinin is found to be hereditary dependent, this could be also helpful in better parentage selection.

Keywords

Conglutinin Nilgai Buffalo Haemagglutination Bovine herpesvirus 

Notes

Acknowledgements

The authors are thankful to the Director, Indian Veterinary Research Institute, Izatnagar for providing necessary facilities to carry out the work.

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflict of interest.

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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • D. Ramesh
    • 1
  • S. Chandra Mohan
    • 2
  • Mohini Saini
    • 3
  • Sasmita Barik
    • 3
  • M. Shynu
    • 4
  • Asit Das
    • 5
  • Anil Kumar Sharma
    • 2
  • Vinod Kumar Chaturvedi
    • 6
  • Praveen Kumar Gupta
    • 7
  1. 1.Department of Veterinary BiochemistryVeterinary CollegeGadagIndia
  2. 2.Centre for WildlifeIndian Veterinary Research InstituteIzzatnagarIndia
  3. 3.Division of BiochemistryIndian Veterinary Research InstituteIzzatnagarIndia
  4. 4.Department of Physiology and BiochemistryVeterinary CollegePookodeIndia
  5. 5.Division of Animal NutritionIndian Veterinary Research InstituteIzzatnagarIndia
  6. 6.Division of Biological ProductsIndian Veterinary Research InstituteIzzatnagarIndia
  7. 7.Division of Veterinary BiotechnologyIndian Veterinary Research InstituteIzzatnagarIndia

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