Symbiotic Response of Drought Tolerant Soybean Varieties, DSR 2 and DSR 12 to Different Arbuscular Mycorrhizal Fungi

  • R. Ashwin
  • D. J. Bagyaraj
  • B. Mohan Raju
Research Article


Arbuscular mycorrhizal fungi (AMF) are known for symbiotic association with crop plants. A glasshouse experiment was conducted to screen and select the efficient AMF for inoculating two drought tolerant soybean varieties DSR 2 and DSR 12. Screening was done with 10 different species of AMF and plant parameters like plant height, stem diameter, biovolume index, total leaf area, dry biomass, P concentration, and mycorrhizal parameters like root colonization, spore number in the root zone soil were recorded according to the standard procedures. Based on the improved plant parameters viz., plant dry biomass, pod and seed yield, total leaf area and P uptake, it was concluded that Rhizophagus fasciculatus is the best AMF for inoculating DSR 2 variety and Ambispora leptoticha is the best for inoculating DSR 12 variety.


Ambispora leptoticha AMF Plant growth response Rhizophagus fasciculatus Soybean 



The authors are thankful to ICAR-NBAIM for financial assistance under the scheme AMAAS (Grant No. NBAIM/AMAAS/2014-17/PF/48) to carry out the present study.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest to publish this manuscript.


  1. 1.
    Agarwal DK, Billore SD, Sharma AN, Dupare BU, Srivastava SK (2013) Soybean: introduction, improvement, and utilization in India: problems and prospects. Agric Res 2:293–300CrossRefGoogle Scholar
  2. 2.
    Bisaliah S (1988) Soybean development in India. CGPRT No. 5Google Scholar
  3. 3.
    FAO Data (2010) Greening agriculture in India: an overview of opportunities and constraints. Accessed 19 Feb 2011
  4. 4.
    Bagyaraj DJ, Sharma MP, Maiti D (2015) Phosphorus nutrition of crops through arbuscular mycorrhizal fungi. Curr Sci 108:1288–1293Google Scholar
  5. 5.
    Bagyaraj DJ (2014) Mycorrhizal fungi. Proc Indian Natl Sci Acad 80:415–428CrossRefGoogle Scholar
  6. 6.
    Raghavendra Kumar M, Ashwin R, Bagyaraj DJ (2016) Screening arbuscular mycorrhizal fungi in order to select the best for alleviating wilt disease complex of capsicum. Proc Natl Acad Sci Ind Sect B Biol Sci. Google Scholar
  7. 7.
    Mathimaran N, Sharma MP, Mohan Raju B, Bagyaraj DJ (2017) Arbuscular mycorrhizal symbiosis and drought tolerance in crop plants. Mycosphere 8:361–376CrossRefGoogle Scholar
  8. 8.
    Hemlata C, Bagyaraj DJ, Thilagar G, Ravi JE (2012) Plant growth response of French bean to arbuscular mycorrhizal fungi. J Soil Biol Ecol 32:50–56Google Scholar
  9. 9.
    Srinivasan M, Ashwin R, Bagyaraj DJ (2012) Symbiotic response of amla (Emblica officinalis Gaertn.) to different arbuscular mycorrhizal fungi. J Soil Biol Ecol 32:37–44Google Scholar
  10. 10.
    Sreeramulu (1996) Selection of efficient VA mycorrhiza for inoculating cardamom nurseries. Ph.D. thesis (Agricultural Microbiology), University of Agricultural Sciences, BangaloreGoogle Scholar
  11. 11.
    Thilagar (2015) Development of microbial consortia for sustainable cultivation of chilly. Ph.D. thesis (Microbiology), PRIST University, ThanjavurGoogle Scholar
  12. 12.
    Thilagar G, Bagyaraj DJ (2015) Influence of different arbuscular mycorrhizal fungi on growth and yield of chilly. Proc Natl Acad Sci India Sect B Biol Sci 85:71–75CrossRefGoogle Scholar
  13. 13.
    Hatchell GE (1985) Production of bare root seedlings. In: Proceedings of 3rd bio south SI research conference, pp 395–402Google Scholar
  14. 14.
    Jackson ML (1973) Soil chemical analysis. Prentice Hall of India, New DelhiGoogle Scholar
  15. 15.
    Gerdemann JW, Nicolson TH (1963) Spores of mycorrhizal Endogone species extracted from soil by wet-sieving and decanting. Trans Br Mycol Soc 46:235–244CrossRefGoogle Scholar
  16. 16.
    Philips JH, Hayman DS (1970) Improved procedures for cleaning roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161CrossRefGoogle Scholar
  17. 17.
    Giovannetti M, Mosse B (1980) An evaluation of techniques to measure vesicular arbuscular infection in roots. New Phytol 84:489–500CrossRefGoogle Scholar
  18. 18.
    Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. Wiley, New YorkGoogle Scholar
  19. 19.
    Ulfath Jaiba J, Balakrishna AN, Bagyaraj DJ, Arpana J (2006) Screening of an efficient AM fungus for inoculating Piper longum Linn. J Soil Biol Ecol 26:109–116Google Scholar
  20. 20.
    Soram IS, Dutta BK, Jha DK (2012) Growth response of arbuscular mycorrhizal (Glomus sp.) fungal inoculation in Gmelina arborea (Linn). Assam Univ J Sci Technol 9:11–17Google Scholar
  21. 21.
    Bagyaraj DJ, Kehri HK (2012) AM fungi: importance, nursery inoculation and performance after outplanting. In: Bagyaraj DJ, Tilak KVBR, Kehri HK (eds) Microbial diversity and functions. New India Publishing Agency, New Delhi, pp 641–668Google Scholar
  22. 22.
    Meghvansi MK, Prasad K, Harwani D, Mahna SK (2008) Response of soybean cultivars toward inoculation with three arbuscular mycorrhizal fungi and Bradyrhizobium japonicum in the alluvial soil. Eur J Soil Biol 44:316–323CrossRefGoogle Scholar
  23. 23.
    Pushpa KK, Lakshman HC (2014) Screening of efficient AM fungus to improve plant growth yield and biomass production of tomato (Solanum lycopersicum L). Sci Report 4:08–13Google Scholar
  24. 24.
    Mondal T, Datta JK, Mondal NK (2015) Chemical fertilizer in conjunction with biofertilizer and vermicompost induced changes in morpho-physiological and bio-chemical traits of mustard crop. J Saudi Soc Agric Sci 16:135–144Google Scholar
  25. 25.
    Gupta ML, Janarthanan KK (1991) Mycorrhizal association of Glomus aggregatum with Palmarosa enhances, growth and biomass. Plant Soil 131:261–264CrossRefGoogle Scholar
  26. 26.
    Gogoi P, Singh RK (2011) Differential effect of some arbuscular mycorrhizal fungi on growth of Piper longum L (Piperaceae). Indian J Sci Technol 4:119–125Google Scholar
  27. 27.
    Al-Karaki GN (2006) Nursery inoculation of tomato with arbuscular mycorrhizal fungi and subsequent performance under irrigation with saline water. Sci Hortic 109:1–7CrossRefGoogle Scholar
  28. 28.
    Ortas I (2010) Effect of mycorrhiza application on plant growth and nutrient uptake in cucumber production under field conditions. Span J Agric Res 8:116–122CrossRefGoogle Scholar
  29. 29.
    Berruti A, Lumini E, Balestrini R, Bianciotto V (2016) Arbuscular mycorrhizal fungi as natural biofertilizers: let’s benefit from past successes. Front Microbiol. PubMedPubMedCentralGoogle Scholar
  30. 30.
    Rajan SK, Bagyaraj DJ, Arpana J (2005) Screening for an efficient arbuscular mycorrhizal fungi for inoculating Albizzia lebbeck. J Soil Biol Ecol 25:116–123Google Scholar
  31. 31.
    Wang X, Pan Q, Chen F, Yan X, Liao H (2011) Effects of co-inoculation with arbuscular mycorrhizal fungi and rhizobia on soybean growth as related to root architecture and availability of N and P. Mycorrhiza 21:173–181CrossRefPubMedGoogle Scholar
  32. 32.
    Helgason T, Merryweather JW, Denison J, Wilson P, Young JPW, Fitter AH (2002) Selectivity and functional diversity in arbuscular mycorrhizas of co-occurring fungi and plants from a temperate deciduous woodland. J Ecol 90:371–384CrossRefGoogle Scholar
  33. 33.
    Vandenkoornhuyse P, Ridgway KP, Watson IJ, Fitter AH, Young JPW (2003) Co-existing grass species have distinctive arbuscular mycorrhizal communities. Mol Ecol 12:3085–3095CrossRefPubMedGoogle Scholar
  34. 34.
    Bagyaraj DJ (2011) Microbial biotechnology for sustainable agriculture, horticulture and forestry. New India Publishing Agency, New DelhiGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.PRIST UniversityVallam, ThanjavurIndia
  2. 2.Centre for Natural Biological Resources and Community Development (CNBRCD)BangaloreIndia
  3. 3.Department of Crop PhysiologyUniversity of Agricultural Sciences, GKVKBangaloreIndia

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