The Wax Encapsulated Organophosphate Hydrolase (WEO) Displays Prolonged Shelf-Life and Effective in Decontamination of Methyl Parathion and Sarin
- 31 Downloads
The recombinant organophosphate hydrolase (OPH) purified from E. coli was used to encapsulate in nonionic wax. The wax encapsulated OPH (WEO) appeared in uniformly sized particles. The WEOs were used to assess OPH stability at different storage conditions. Unlike the free OPH, which has lost most of its activity within 2 weeks, the WEO particles have shown dramatic increase in shelf-life. The lyophilized WEO particles stored at 4 °C have retained complete activity for more than a year. Only 10% of total activity was lost when stored at room temperature. The foams and gelatin pads prepared using WEO particles successfully hydrolyzed OP nerve agent, sarin.
KeywordsOrganophoshpate hydrolase (OPH) Nerve agents Organophosphate insecticides Wax encapsulated OPH (WEO)
Department of Biotechnology is supported by Andhra Pradesh Netherlands Biotechnology programme and Department of Biotechnology, Govt. of India, is acknowledged.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest to publish this manuscript.
- 5.Cronce DT (1999) Chemical warfare agent decontamination solution. U.S. patent 5859064Google Scholar
- 14.Thakur S, Venkateswar Reddy M, Siddavattam D, Paul AK (2012) A fluorescence based assay with pyranine labeled hexa-histidine tagged organophosphorus hydrolase (OPH) for determination of organophosphates. Sens Actuators B Chem 163:153–158. https://doi.org/10.1016/j.snb.2012.01.024 CrossRefGoogle Scholar
- 16.Rastogi VK, Cheng TC, DeFrank JJ (2002) One-step purification process for organophosphorus hydrolase enzyme. US Patent 6Google Scholar
- 17.LeJeune KE, Mesiano AJ, Bower SB et al (1997) Dramatically stabilized phosphotriesterase-polymers for nerve agent degradation. Biotechnol Bioeng 54:105–114. https://doi.org/10.1002/(SICI)1097-0290(19970420)54:2<105:AID-BIT2>3.0.CO;2-P CrossRefPubMedGoogle Scholar