Isolation and Characterization of Endophytic Bacteria from Piper longum

Abstract

Endophytes are non-pathogenic microorganisms that reside within internal tissues of plant, without causing any apparent symptoms of infection. In an attempt to identify endophytes from Piper longum, the Indian long pepper, a combination of conventional and molecular approaches was used. Using culture-dependent approach, six different bacterial isolates were obtained from various surface-sterilized parts (roots, nodes, internodes, petioles, leaves and spikes) of the plant. In general, roots harboured maximum concentration of endophytic bacterial isolates, while leaves contained the minimum levels. These endophytes were analyzed on the basis of colony morphology and biochemical characteristics. Based on the results obtained after BLAST search of 16S rDNA sequence in NCBI database, the endophytes isolated from Piper longum showed highest similarity to Endophytic bacteria 135L-3, Enterobacter sp. SQ6-43, Bacillus casamancensis strain TN3, Alishewanella sp. JS-30, Bacterium B28 and Enterobacter ludwigii strain g45. Most of the identified bacteria belong to the phylum Proteobacteria and Firmicutes, whose members have been reported to act as growth-promoting bacteria in other plant species. Some of these endophytes tested positive for the ability to produce indole-3-acetic acid and hydrogen cyanide, suggesting their potential roles in plant growth promotion and biological control against pathogens, respectively.

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Acknowledgements

One of the authors wishes to acknowledge the University Grants Commission for non-SAP Fellowship. The authors also acknowledge the Director, Dayalbagh Educational Institute, for the financial support to carry out this work.

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Correspondence to Prem Kumar Dantu.

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Significance Statement

The endophytic bacteria of Piper longum have plant growth-promoting and biocontrol properties. Further studies in this direction would unravel their mode of transmission, co-evolution and bioprospecting for economically important compounds.

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Mintoo, M.N., Mishra, S. & Dantu, P.K. Isolation and Characterization of Endophytic Bacteria from Piper longum. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 1447–1454 (2019). https://doi.org/10.1007/s40011-018-01064-8

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Keywords

  • Piper longum
  • Endophytic bacteria
  • Medicinal plant
  • 16S rRNA sequencing
  • Microbial diversity