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Assessment of Circulating Biochemical Markers in Mice Receiving Cinnamon and Glycyrrhizin Under Carbon Tetrachloride Induced Hepatic Injury

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The study evaluated the hepatoprotective activity of plant extracts of cinnamon and glycyrrhizin in distinct dosage ways to minimize the oxidative stress induced by carbon tetrachloride (CCl4) in BALB/cJ inbred albino mice. Fifteen albino mice were divided into five groups, each group containing three mice. Group A was referred as positive control while group B, C, D and E were injected intraperitoneally with 1 mL/kg body weight of CCl4 twice a week for 1 month. Group C and D were treated orally with isolated extracts of cinnamon @50 mg/kg and glycyrrhizin @50 mg/kg respectively on daily basis for 1 month. However, group E was treated orally with combination dose of cinnamon @50 mg/kg + glycyrrhizin @50 mg/kg body weight. The increase in the levels of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), triglyceride (TG), malondialdehyde (MDA) and glucose were recorded in CCl4 induced liver injury in mice while there is decrease in the levels of total protein (TP), reduced glutathione (GSH), Superoxide dismutase (SOD) and catalase (CAT) in CCL4 intoxicated mice. Isolated therapy of plant extracts of cinnamon and glycyrrhizin decreased the levels of ALT, AST, ALP, MDA, TG and glucose whereas increase in TP, GSH, SOD and CAT was observed in plant extracts treated mice. The best restoration of all the above said parameters near to control was observed in group of mice treated with combination dose of cinnamon and glycyrrhizin @50 mg/kg. Therefore, the present study declared the antioxidative, anti-inflammatory and hepatoprotective activity of standardized extracts of cinnamon and glycyrrhizin and their potent defensive property.

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Abbreviations

CCl4 :

Carbon tetrachloride

ROS:

Reactive oxygen species

RNS:

Reactive nitrogen species

ALT:

Alanine transaminase

AST:

Aspartate transaminase

ALP:

Alkaline phosphatase

TG:

Triglyceride

MDA:

Malondialdehyde

TP:

Total protein

GSH:

Reduced glutathione

SOD:

Superoxide dismutase

CAT:

Catalase

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Acknowledgements

This research work was funded and supported by Institute of Molecular Biology and Biotechnology, and Department of Pharmacy, The University of Lahore, Lahore, Pakistan. This project was partially supported by the National Plan for Science, Technology and Innovation (MAARIFAH) – King Abdulaziz City for Science and Technology - the Kingdom of Saudi Arabia – award number (10-BIO1073-03, 10-BIO1258-03 and 08-MED120-03).

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Authors and Affiliations

  1. Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, PO BOX 80216, Jeddah, 21589, Kingdom of Saudi Arabia

    Mahmood Rasool, Shakeel Ahmed Ansari, Mohammed Hussain Al-Qahtani & Sajjad Karim

  2. Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan

    Arif Malik & Sadia Saleem

  3. Department of Pharmacy, The University of Lahore, Lahore, Pakistan

    Javed Iqbal

  4. Department of Biotechnology and Informatics, BUITEMS, Quetta, Pakistan

    Muhammad Asif

  5. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia

    Mohammed Amjad Kamal

Authors
  1. Mahmood Rasool
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  2. Arif Malik
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  3. Sadia Saleem
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  4. Shakeel Ahmed Ansari
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  5. Javed Iqbal
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  6. Muhammad Asif
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  7. Mohammed Amjad Kamal
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  8. Mohammed Hussain Al-Qahtani
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  9. Sajjad Karim
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Correspondence to Sajjad Karim.

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This research work is approved by local ethical committee of university and the authors further declare that presently this manuscript is not submitted to any journal for publication consideration.

Additional information

Significance Statement The present study evaluated the medicinal properties of glycyrrhizin and cinnamon extracts and found that they possess the antioxidative properties that help in the recovery of liver injury by lowering the lipid peroxidation and stabilizing the altered serum biomarkers.

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Rasool, M., Malik, A., Saleem, S. et al. Assessment of Circulating Biochemical Markers in Mice Receiving Cinnamon and Glycyrrhizin Under Carbon Tetrachloride Induced Hepatic Injury. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 105–111 (2019). https://doi.org/10.1007/s40011-017-0920-6

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  • DOI: https://doi.org/10.1007/s40011-017-0920-6

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