The increasing heavy metal pollution in the agro-ecosystem has become a serious concern worldwide. These metals do not decay in the nature and become toxic to the plants, animals and human beings when exceed specific thresholds. Anthropogenic input of heavy metals in agricultural land includes industrial and agricultural disposal, waste incineration and urban effluent of wastewater. Phytoremediation, by using metal-accumulating plants like Brassica sp., including Indian mustard (Brassica juncea) for toxic metal removal from soil has been proposed as a possible solution to this problem. Phytoremediation is a cost effective and nondestructive, but the challenges in the fast extraction of heavy metals by Indian mustard include initial slow growth, ability to extract some particular metal only, poor bioavailability of heavy metals in soils and non-compartmentalization within plant parts. Addition of organic matter, organic chelates, soil amendments, adoption of suitable cropping systems, intercrops and fertilizer selection can enhance the phytoremediation capacity of Indian mustard. Growing Indian mustard with these agronomic interventions can augment the ability to absorb, uptake and concentrate heavy metal under contaminating soils.
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Ministry of Agriculture (2015) Agricultural statistics at a glance. Department of Agricultural and Cooperation, Krishi Bhawan, New Delhi, p 2015
Jabeen R, Ahmad A, Iqbal M (2009) Phyto-remediation of heavy metals: physiological and molecular aspects. Bot Rev 75:339–364
Claus D, Dietze H, Gerth A, Grosser W, Hebner A (2007) Application of agronomic practice improves phytoextraction on a multipolluted site. J Environ Eng Landscape Manag 15:208–212
Mohammad Naser H, Shil NC, Mahmud NU, Rashid H, Hossain KM (2009) Lead, cadmium and nickel contents of vegetables grown in industrially polluted and non-polluted areas of Bangladesh. Bangladesh J Agric Res 34:545–554
Diwan H, Ahmad A, Iqbal M (2008) Genotypic variation in the phytoremediation potential of Indian mustard for chromium. Environ Manag 41:734–741
Bajaj M, Eiche E, Neumann T, Winter J, Gallert C (2011) Hazardous concentrations of selenium in soil and groundwater in north-west India. J Hazard Mater 189:640–646
Rajappa B, Manjappa S, Puttaiah ET (2010) Monitoring of heavy metal concentration in groundwater of Hakinaka Taluk, India. Contemp Eng Sci 3:183–190
Patra M, Sharma A (2000) Mercury toxicity in plants. Bot Rev 66:379–422
Linshy VN, Saraswa R, Sujata RK, Nigam R (2013) Experiment to decipher the effect of heavy metal cadmium on coastal benthic foraminifera pararotalianipponica (ASANO). J Palaeontol Soc India 58:205–211
Vanita C, Piar C, Avinash N, Kaur KJ, Pakade YB (2014) Evaluation of heavy metals contamination and its genotoxicity in agricultural soil of Amritsar, Punjab, India. Int J Res Chem Environ 4:20–28
Arunachalam P, Kannan P, Prabukumar G, Govindaraj M (2013) Zinc deficiency in Indian soils with special focus to enrich zinc in peanut. Afr J Agric Res 8:6681–668812
Ma LQ, Komar KM, Tu C, Zhang WH, Cai YE, Kennelley D (2001) A fern that hyperaccumulates arsenic: a hardy versatile, fast growing plant helps to remove arsenic from contaminated soils. Nature 4:409–579
Rathore SS, Kapila S, Premi OP, Kandpal BK (2013) Water-use efficiency, productivity, photosynthesis and sustainability of pressurized irrigation systems for Indian mustard (Brassica juncea L. Czernj and Cosson) under semi-arid conditions of Rajasthan. Res Crops 14:140–150
Ansari MK, Ahmad A, Umar S, Zia MH, Iqbal M, Owens G (2015) Genotypic variation in phytoremediation potential of Indian mustard exposed to heavy metal stress: a hydroponic study. Int J Phytoremediat 17:135–144
Hall JL (2002) Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 53:1–11
Boominathan R, Doran PM (2003) Cadmium tolerance and antioxidative defenses in hairy roots of the cadmium hyper-accumulator, Thlaspi caerulescens. Biotechnol Bioeng 83:158–167
Ma JF, Ueno D, Zhao FJ, McGrath SP (2005) Subcellular localization of Cd and Zn in the leaves of a Cd-hyperaccumulating ecotype of Thlaspi caerulescens. Planta 220:731–736
Mani D, Sharma B, Kumar C, Balak S (2013) Depth-wise distribution, mobility and naturally occurring glutathione based phyto-accumulation of Cd and Zn in sewage-irrigated soil profiles. Int J Environ Sci Technol 10:1167–1180
Kaur Leela G, Adgil K, Sharma S (2015) Phytoextraction based on Indian mustard (Brassica juncea) planted on spiked soil by aliquot amount of lead and nickel. EQA Environ Qual 17:13–23
Yadav SK (2010) Heavy metals toxicity in plants: an overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. South Afr J Bot 76:167–179
Muthukumar N, Maruthamuthu S, Palaniswamy N (2007) Role of cationic and non-ionic surfactants on biocidal efficiency in diesel–water interface. Colloids Surf B Biointerfaces 57:152–160
Sahay S, Inam A, Iqbal H, Iqbal S (2013) Growth, physiological and yield response in four oilseed Brassica cultivars under urban wastewater irrigation. Biosci Int 2:45–53
Bauddh K, Singh RP (2009) Genotypic differences in nickel (Ni) toxicity in Indian mustard (Brassica juncea). Pollut Res 28:699–704
Roychoudhury A, Pradhan S, Chaudhuri B, Das K (2012) Phytoremediation of toxic metals and the involvement of Brassica species. In: Naser AR et al (eds) Phytotechnologies remediation of environmental contaminants. CRC Press, Taylor and Francis groups, Boca Raton, pp 219–252
Birringer M, Pilawa S, Flohe L (2002) Trends in selenium biochemistry. Nat Prod Rep 19:693–71825
El Mehdawi AF, Cappa J, Fakra SC, Self J, Pilon-Smits EAH (2012) Interactions of selenium hyperaccumulators and nonaccumulators during cocultivation on seleniferous or nonseleniferous soil—the importance of having good neighbors. New Phytol 194:264–277
Harris J, Schneberg KA, Elizabeth A, Smits P (2014) Sulfur–selenium–olybdenum interactions distinguish selenium hyperaccumulator Stanleyapinnata from non-hyperaccumulator Brassica juncea (Brassicaceae). Planta 239:479–491
Zenk MH (1996) Heavy metal detoxification in higher plants: a review. Gene 179:21–30
Singh S, Sinha S (2005) Accumulation of metals and its effects in Brassica juncea (L.) Czern. (cv. Rohini) grown on various amendments of tannery waste. Ecotoxicol Environ Saf 62:118–127
Sunita S, Bikram S, Manchanda VK (2015) Phytoremediation: role of terrestrial plants and aquatic macrophytes in the remediation of radio-nuclides and heavy metal contaminated soil and water. Environ Sci Pollut Res 22:946–96230
Papoyan A, Kochian LN (2004) Identification of Thlaspi caerulescens genes that may be involved in heavy metal hyperaccumulation and tolerance: characterization of a novel heavy metal transporting ATPase. Plant Physiol 136:3814–3823
Maiti RK, Hernanadez-Pinero JL, Gonzalez-Oreja JA, Lopez-Santiago D (2004) Plant based bioremediation and mechanisms of heavy metal tolerance of plants: a review. Proc Indian Natl Sci Acad 70:1–12
Shiyab S, Chen J, Han FX, Monts DL, Matta FB, Gu M, Su Y (2009) Phytotoxicity of mercury in Indian mustard (Brassica juncea L.). Ecotoxicol Environ Saf 72:619–625
Chaney RL, Li YMS, Brown L, Homer FA, Malik M, Angle JS (2000) Improving metal hyperaccumulator wild plants to develop commercial phyto-extraction systems: approaches and progress. In: Terry N, Banuelos G, Vangronsveld J (eds) Phytoremediation of contaminated soil and water. Lewis Publishers, Boca Raton, pp 129–158
Premi OP, Kandpal BK, Rathore SS, Shekhawat K, Chauhan JS (2013) Green manuring, mustard residues recycling and fertilizer application affects productivity and sustainability of Indian mustard (Brassica juncea L.) in Indian semi arid tropics. Ind Crops Prod 41:423–429
Jing Y, He Z, Yang X (2007) Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils. J Zhejiang Univ Sci B 8:192–207
Singh G, Brar MS, Malhi SS (2007) Decontamination of chromium by farm yard manure application in spinach grown in two texturally different Cr-contaminated soils. J Plant Nutr 30:289–308
Jadia CD, Fulekar MH (2008) Phytoremediation: the application of vermi-compost to remove zinc, cadmium, copper, nickel and lead by sunflower plant. Environ Eng Manag J 7:547–558
Preeti K (2015) Use of natural organic and synthetic chelating agents for efficient phytoremediation. Int J Enhanc Res Sci Technol Eng 4:99–101
Anwar HM, Pukclai P, da Silva JAT, Fujita M (2012) Molecular mechanism of heavy metal toxicity and tolerance in plants: central role of glutathione in detoxification of reactive oxygen species and methylglyoxal and in heavy metal chelation. J Bot. doi:10.1155/2012/872875
Luo ZB, Janz D, Jiang X, Gobel C, Wildhagen H, Tan Y (2009) Upgrading root physiology for stress tolerance by ectomycorrhizas: insight from metabolite and transcriptional profiling into reprogramming for sizes anticipation. Plant Physiol 151:1902–1912
Whitfield L, Richards AJ, Rimmer DL (2003) Effects of mycorrhizal colonization on Thymus polytrichus from heavy metal-contaminated sites in north England. Mycorrhiza 14:47–54
Ma Y, Rajkumar M, Freitas H (2009) Inoculation of plant growth promoting bacterium Achromobacter xylosoxidans strain Ax10 for the improvement of copper phytoextraction by Brassica juncea. J Environ Manag 90:831–837
Sinha S, Mukherjee SK (2008) Cadmium-induced siderophore production by a high Cd-resistant bacterial strain relieved Cd toxicity in plants through root colonization. Curr Microbiol 56:55–60
Dellamic EL, Cavalca V (2008) Andreoni improvement of Brassica napus growth under cadmium stress by cadmium-resistant rhizobacteria. Soil Biol Biochem 40:74–84
Sheng XF, Xia JJ, Jiang CY, He LY, Qian M (2008) Characterization of heavy metal-resistant endophytic bacteria from rape (Brassica napus) roots and their potential in promoting the growth and lead accumulation of rape. Environ Pollut 156:1164–1170
Sheng XF, Xia JJ (2006) Improvement of rape (Brassica napus) plant growth and cadmium uptake by cadmium-resistant bacteria. Chemosphere 64:1036–1042
Zaidi S, Usmani S, Singh BR, Musarrat J (2006) Significance of Bacillus subtilis strain SJ-101 as a bioinoculant for concurrent plant growth promotion and nickel accumulation in Brassica juncea. Chemosphere 64:991–997
Liphadzi MS, Kirkham MB, Mankin KR, Paulsen GM (2003) EDTA-assisted heavy metal uptake by poplar and sunflower grown at a long-term sewage sludge farm. Plant Soil 257:171–8249
Wenger K, Kaiser A, Gupta SK, Furre G, Schulin R (2002) The comparison of NTA and elemental sulfur as potential soil amendments. Soil Sedim Contam 1:655–672
Shekhawat K, Rathore SS, Premi OP, Kandpal BK, Chauhan JS (2012) Advances in agronomic management of Indian Mustard (Brassica juncea (L.) Czernj. Cosson): an overview. Int J Agron 2012:1–14
Olson PE, Castro A, Joern M, DuTeau NM, Pilon-Smits E, Reardon KF (2008) Effects of agronomic practices on phytoremediation of an aged PAH-contaminated soil. J Environ Qual 37:1439–144653
Susarla S, Medina VF, Mc Cutcheon SC (2002) Phytoremediation: an ecological solution to organic chemical contamination. Ecol Eng 18:647–65854
Smical AI, Hotea V, Oros V, Juhasz J, Pop E (2008) Studies on transfer and bioaccumulation of heavy metals from soil into lettuce. Environ Eng Manag J 7:609–615
Kashem MA, Singh BR (2002) The effect of fertilizer additions on the solubility and plant-availability of Cd, Ni and Zn in soil. Nutr Cycl Agroecosyst 62:287–296
Kayser A, Wenger K, Keller A, Attinger W, Felix HR, Gupta SK, Schulin R (2003) Enhancement of phytoextraction of Zn, Cd and Cu from calcareous soil: the use of NTA and sulfur amendments. Environ Sci Technol 34:1778–1783
Zhuang P, Ye ZH, Lan CY, Xie ZW, Shu WS (2005) Chemically assisted phytoextraction of heavy metal contaminated soils using three plant species. Plant Soil 276:153–162
Ramsay MA, Swannell RPJ, Shipton WA, Duke NC, Hill RT (2000) Effect of bioremediation on the microbial community in oiled mangrove sediments. Marine Pollut Bull 41:413–419
Dhillon SK, Dhillon KS (2009) Phytoremediation of selenium-contaminated soils: the efficiency of different cropping systems. Soil Use Manag 25:441–45360
Rathore SS, Shekhawat K, Premi OP, Kandpal BK, Singh D (2015) Improved agronomic practices for cultivation of rapeseed-mustard in India. ICAR-Directorate of Rapeseed-Mustard Research Sewar, Bharatpur
Kacalkova L, Tlustos P, Szakova J (2009) Phyto-extraction of cadmium, copper, zinc and mercury by selected plants. Plant Soil Environ 55:295–304
Yang L, Kai L, Yong L, Yang Wanqin W, Fuzhong ZP, Jian Z, Chen L, Gao S, Zhang L (2016) Cadmium contamination of soil and crops is affected by intercropping and rotation systems in the lower reaches of the Minjiang River in south-western China. Environ Geochem Health 38:811–82063
Rathore SS, Shekhawat K, Premi OP, Kandpal BK (2012) Major weeds of rapeseed-mustard in India. All India Coordinated Research Project on Rapeseed-Mustard (ICAR) Directorate Rapeseed-Mustard Research, Bharatpur
Fulekar MH, Anamika S, Anwesha Bhaduri M (2009) Genetic engineering strategies for enhancing phytoremediation of heavy metals. Afr J Biotechnol 8:529–535
Ruiz ON, Daniell H (2009) Genetic engineering to enhance mercury phyto-remediation. Curr Opin Biotechnol 20:213–219
All benefits in any form from a commercial party related directly or indirectly to the subject of this manuscript or any of the authors are dully acknowledged. This is a review article; therefore the issue of fund is not applicable. However, the support and guidance rendered by ICAR-IARI is fully acknowledged.
Conflict of interest
The authors declare that they have no conflict of interest.
The study is relevant in the context of rising awareness about organic farming. Phytoremediation and its enhancement through agronomic intervention certainly going to help in detoxifying soils and getting good quality of produce. In many of the areas especially in peri urban and urban, polluted water is being released in agricultural lands. Subsequently its disposal in arable soil is leading to soil sickness and poisoning of edible produce with heavy metals. Phytoremediation is going to help in great-way in addressing these problems.
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Rathore, S.S., Shekhawat, K., Dass, A. et al. Phytoremediation Mechanism in Indian Mustard (Brassica juncea) and Its Enhancement Through Agronomic Interventions. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 419–427 (2019). https://doi.org/10.1007/s40011-017-0885-5
- Bioconcentration factor
- Brassica sp.
- Chelating agent