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Protective Mechanisms of Quercetin on Cisplatin Induced Oxidative Damage in Hepatic Tissue of Wistar Rats

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Abstract

The present study was undertaken to investigate the cisplatin (cDDP) induced oxidative damage in hepatic tissue of wistar rats and mechanisms of protection by quercetin. A total of 24 wistar rats were randomly divided into four groups with six animals in each. Group I served as control, group II received cDDP (12 mg kg−1 body weight) and group III quercetin (50 mg kg−1 body weight) intra-peritoneally. Group IV received quercetin 6 h prior to cDDP administration intra-peritoneally. Administration of cDDP in rats resulted in significant (P < 0.05) elevation of plasma hepatic biomarkers, reduction of antioxidant system and marked histopathological alterations indicating acute hepatotoxicity. Treatment with quercetin prior to cDDP administration prevented hepatic dysfunctions as indicated by alterations in hepatic biomarkers, alleviated enzymatic and non-enzymatic components of the antioxidant system with reduced histopathological changes in hepatic tissue. The results suggest that cDDP induced hepatic damage is due to imbalance in oxidant and antioxidant system of hepatic tissue. The pretreatment with quercetin attenuated the oxidative damage induced by cDDP in hepatic tissue and this hepatoprotective effect of quercetin may be due to its direct scavenging of free radicals and/or enhancing antioxidant defense system of hepatic tissue in wistar rats.

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Acknowledgements

The authors thank to the Dean, Faculty of Veterinary Science and Animal Husbandry, R S Pura, Jammu for providing necessary facilities for conducting the research.

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Authors and Affiliations

  1. Division of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, R S Pura, 181102, India

    Pawan K. Verma, Rajinder Raina, Shahid Prawez & Mudasir Sultana

  2. Division of Veterinary Public Health and Epidemiology, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, R S Pura, 181102, India

    Maninder Singh

  3. Division of Pathology, Indian Veterinary Research Institute, Izzatnagar, Bareilly, UP, 243122, India

    Pawan Kumar

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  1. Pawan K. Verma
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Correspondence to Pawan K. Verma.

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Significance statement

Cisplatin is an effective anticancer drug for the treatment of solid tumors with a limitation of potent nephrotoxicity and hepatotoxicity. To reduce its toxicity by using some natural compounds (quercetin) along with cisplatin can be an effective way for therapy. For this it is essential to know protective mechanisms for better utilization of natural compounds.

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Verma, P.K., Raina, R., Prawez, S. et al. Protective Mechanisms of Quercetin on Cisplatin Induced Oxidative Damage in Hepatic Tissue of Wistar Rats. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 1399–1407 (2018). https://doi.org/10.1007/s40011-017-0877-5

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  • DOI: https://doi.org/10.1007/s40011-017-0877-5

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