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Assessment of immune response in post-probiotic treated Litopenaeus vannamei challenged with Vibrio harveyi

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences volume 88, pages 797–802 (2018)Cite this article

Abstract

Immunological responses of Litopenaeus vannamei fed with two probiotic bacteria, Bacillus licheniformis and Bacillus megaterium were evaluated at a post probiotic treatment scenario. This shrimp fed with the probiotics individually and in combination for 60 days was fed with normal feed and the immune response was evaluated at the 10th day post probiotic treatment (PPT). The immune response was reduced in all the treatments during the post probiotic treatment even though it was higher than the control. The probiotic treated shrimps were challenged with Vibrio harveyi after 5 days PPT and the immunological evaluation were carried out at the 10th day PPT. Probiotic fed shrimps showed significantly higher survival and immunological response than the control. Among the treatments, combination of the two probiotics enhanced the non-specific immunity significantly in the shrimps than the individual probiotic.

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References

  1. Anderson DP (1992) Immunostimulants, adjuvants and vaccine carriers in fish: application to aquaculture. Annu Rev Fish Dis 2:281–307

    Article  Google Scholar 

  2. Sung HH, Yang YL, Song YL (1996) Enhancement of microbicidal activity in the tiger shrimp Penaeus monodon via immunostimulation. J. Crustac. Biol. 16:278–284

    Article  Google Scholar 

  3. Li K, Zheng T, Tian Y, Xi F, Yuan J, Zhang G, Hong H (2007) Beneficial effects of Bacillus licheniformis on the intestinal microflora and immunity of the white shrimp, Litopenaeus vannamei. Biotechnol. Lett. 29:525

    Article  PubMed  CAS  Google Scholar 

  4. Tseng DY, Ho PL, Huang SY, Cheng SC, Shiu YL, Chiu CS, Liu C-H (2009) Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20. Fish Shellfish Immunol 26:339–344

    Article  PubMed  CAS  Google Scholar 

  5. Rengpipat S, Rukpratanporn S, Piyatiratitivorakul S, Menasaveta P (2000) Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11). Aquaculture 191:271–288

    Article  CAS  Google Scholar 

  6. Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol 64:548

    Article  CAS  Google Scholar 

  7. Moriarty DJW (1997) The role of microorganisms in aquaculture ponds. Aquaculture 151:333–349

    Article  Google Scholar 

  8. Moriarty DJW (1998) Control of luminous Vibrio species in penaeid aquaculture ponds. Aquaculture 164:351–358

    Article  Google Scholar 

  9. Kumar A, Suresh Babu PP, Roy SD, Razvi SSH, Charan R (2014) Synergistic effects of two probiotic bacteria on growth, biochemical, and immunological responses of Litopenaeus vannamei (Boone, 1931). Isr J Aquacult 66:1009–1017

    Google Scholar 

  10. Sritunyalucksana K, Gangnonngiw W, Archakunakorn S, Fegan D, Flegal T (2005) Bacterial clearance rate and a new differential hemocyte staining method to assess immunostimulant activity in shrimp. Dis Aquat Org 63:89–94

    Article  PubMed  Google Scholar 

  11. Tanner C, Burnett L, Burnett K (2006) The effects of hypoxia and pH on phenoloxidase activity in the Atlantic blue crab, Callinectes sapidus. Comp Biochem Physiol A 144:218–223

    Article  CAS  Google Scholar 

  12. Secombes CJ (1996) The nonspecific immune system: cellular defenses. In: Iwama G, Nakanishi T (eds) The fish immune system: organism, pathogen, and environment. Academic Press, San Diego, pp 63–103

    Chapter  Google Scholar 

  13. Stasiack AS, Bauman CP (1996) Neutrophil activity as a potent indicator of neromitant analysis. Fish Shellfish Immunol 37:539–542

    Google Scholar 

  14. Reinhold JG (1953) Determination of plasma proteins. In: Standard methods of clinical chemistry, vol 1. Academic press, New York, p 88

  15. Misra HP, Fridovich I (1972) The role of superoxide anion in the antioxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 217:3170–3175

    Google Scholar 

  16. Gullian M, Thompson F, Rodriguez J (2004) Selection of probiotic bacteria and study of their immunostimulatory effect in Penaeus vannamei. Aquaculture 233:1–14

    Article  Google Scholar 

  17. Wiegertjes GF, Stet RJM, Parmeatier HK, Van Muiswinkel WB (1996) Immunogenetics of disease resistance in fish; a comparable approach developmental and comparative immunology. J Exp Boil 20:365–381

    CAS  Google Scholar 

  18. Sung HH, Yang YL, Song YL (1996) Enhancement of microbicidal activity in Tiger shrimp (Penaeus monodon) via immunostimulation. J Crustac Biol 16:278–284

    Article  Google Scholar 

  19. Itami T, Kubono K, Asano M, Tokushige K, Takeno N, Nishimura H, Kondo M, Takahashi Y (1998) Enhancement of disease resistance of kuruma shrimp, Penaeus japonicus, after oral administration of peptidoglycan derived from Bifidobacterium thermophilum. Aquaculture 164:277–288

    Article  CAS  Google Scholar 

  20. Mou HJ, Jiang XL, Liu SQ, Guan HS (1999) Effects of immunopolysaccharide on the activities of acid phosphatase, alkaline phosphatase and superoxide dismutase in Chlamys farreri. J Ocean Univ Qingdao 29:463–468

    Google Scholar 

  21. Liu H, Li GY (1998) The effect of immunopolysaccharide as a food additive on the penaeid shrimp, Penaeus vannamei. Oceanol Limnol Sin 29:113–118

    Google Scholar 

  22. Vieira FN, Buglione CC, Mouriño JPL, Jatobá A, Martins ML, Schleder DD, Andreatta ER, Barraco MA, Vinatea LA (2010) Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi. Arq Bras Med Vet Zootec 62:631–638

    Article  Google Scholar 

  23. Van DeB CBT, Botterblom MHA, Taverne N (2002) The roles of haemocytes and the lymphoid organ in the clearance of injected Vibrio bacteria in Penaeus monodon shrimp. Fish Shellfish Immunol 13:293–309

    Article  Google Scholar 

  24. Jiravanichpaisal P, Lee BL, Söderhäll K (2006) Cell-mediated immunity in arthropods, Hematopoiesis, coagulation, melanization and opsonization. Immunobiology 211:213–236

    Article  PubMed  CAS  Google Scholar 

  25. Fridovich I (1995) Superoxide radical and superoxide dismutases. Annu Rev Biochem 64:97–112

    Article  PubMed  CAS  Google Scholar 

  26. Sharp GJE, Secombes CJ (1993) The role of reactive oxygen species in the killing of the bacterial fish pathogen Aeromonas salmonicida by rainbow trout macrophages. Fish Shellfish Immunol 3:119–129

    Article  Google Scholar 

  27. Thompson FL, Abreu PC, Cavalli R (1999) The use of microorganisms as food source for Penaeus paulensis, larvae. Aquaculture 174:139–153

    Article  Google Scholar 

  28. Verschuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotics bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  29. Ochoa-Solano LJ, Olmos-Soto J (2006) The functional property of Bacillus for shrimps feeds. Food Microbiol 23:519–525

    Article  CAS  Google Scholar 

  30. Nakayama T, Lu H, Nomura N (2009) Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp. Lett Appl Microbiol 49:679–684

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to Dr. W. S. Lakra, The Director, Central Institute of Fisheries Education, Mumbai for the continuous encouragement to carry out the research work.

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Authors and Affiliations

  1. National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Telibagh, Lucknow, UP, 226 002, India

    Aditya Kumar

  2. Central Marine Fisheries Research Institute, West Hill, Calicut, 673005, Kerala, India

    P. P. Suresh Babu

  3. Central Island Agriculture Research Institute, Port Blair, Andaman and Nicobar Island, India

    S. D. Roy

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  1. Aditya Kumar
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  2. P. P. Suresh Babu
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  3. S. D. Roy
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Correspondence to Aditya Kumar.

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Kumar, A., Suresh Babu, P.P. & Roy, S.D. Assessment of immune response in post-probiotic treated Litopenaeus vannamei challenged with Vibrio harveyi . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 797–802 (2018). https://doi.org/10.1007/s40011-016-0818-8

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  • DOI: https://doi.org/10.1007/s40011-016-0818-8

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