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Extracellular Lipase Purification from a Marine Planomicrobium sp. MR23K and Productivity Optimization in a Pilot-Scale Submerged Bioreactor

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

A halotolerant Planomicrobium species with remarkable lipase activity, isolated from the sediments of a mangrove, was investigated for the lipase purification, yield and production improvement. The enzyme purification was increased by 12.86-folds using a two-step purification process by ammonium sulfate precipitation and gel filtration chromatography. Subsequently, a submerged fermentation was optimized for the maximum lipase production using a response surface method (RSM). Several variables, comprising 8 nutritional variables along with pH, temperature and agitation speed were screened for the effective ones by Placket–Burman design (PBD). The effective variables were further examined for their optimum levels for the enzyme production using a central composite design (CCD). NaCl (1.28 % w/v), glucose (1.20 % w/v), olive oil (1.35 % w/v), peptone (0.69 % w/v) and pH (7.8) were found to be effective in maximizing the enzyme productivity in a lab scale (10.08 U/ml of cell-free supernatant) and a 10 l fermenter (9.89 U/ml). The results suggested that the model is accurate and consistent in predicting the best condition. Therefore, the strain under this specific fermentation condition promises a good prospect for a large-scale lipase production.

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Acknowledgments

The authors are very thankful of Dr. Kavyani for helpful advices and also appreciate of all postgraduate students in Shahid Beheshti University for scientific suggestions about statistical analysis and fermentation method.

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Authors and Affiliations

  1. School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

    Manouchehr Teymouri

  2. Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Manouchehr Teymouri & Abdolrazagh Marzban

  3. Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Maryam Karkhane

  4. Department of Biology, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran

    Farhad Gilavand

  5. Immunogenetics Research Center, Department of Physiology and Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

    Javad Akhtari

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  1. Manouchehr Teymouri
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  2. Maryam Karkhane
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Correspondence to Abdolrazagh Marzban.

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Teymouri, M., Karkhane, M., Gilavand, F. et al. Extracellular Lipase Purification from a Marine Planomicrobium sp. MR23K and Productivity Optimization in a Pilot-Scale Submerged Bioreactor. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 739–746 (2018). https://doi.org/10.1007/s40011-016-0812-1

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  • DOI: https://doi.org/10.1007/s40011-016-0812-1

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