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In vitro Evaluation of Insecticides, Bio-Fungicide and Bio-Fertilizer for Strategic and Eco-Friendly Combinatorial Seed Treatments in Chickpea

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Use of seed treatments in Integrated Pest Management of major field crops has increased considerably over the past few years. However, field recommendations are seldom supported by in vitro assays screening the suitability of the pesticides for seed treatment. Hence, the present investigation was undertaken to assess the suitability of three insecticides as imidacloprid (600 FS), fipronil (5 FS) and chlorpyriphos (20 EC) for seed treatment in chick pea as well as to verify the compatibility of identified doses of test-insecticides, along with biofertilizer (Mesorhizobium ciceri @ 25 g/kg seed) and in combination with biofungicide (Trichoderma harzianum @ 4 g/kg seed). Ten treatments were evaluated in insecticidal studies viz. control and three treatments of each insecticides i.e. nine treatments) and eight treatments were evaluated in compatibility study. Based on the results obtained, the authors advocate appropriate IPM-compatible and eco-friendly seed treatment tactics, viz., (1) imidacloprid @ 5 ml/kg + Mesorhizobium, (2) imidacloprid @ 5 ml/kg + Mesorhizobium @ 25 g/kg seed + Trichoderma @ 4 g/kg seed, (3) fipronil @ 5 ml/kg + Mesorhizobium @ 25 g/kg seed, and (4) fipronil @ 5 ml/kg + Mesorhizobium @ 25 g/kg seed + Trichoderma @ 4 g/kg seed. These recommendations may be tested for the field level efficacy and the insecticides tested hereby are essentially termiticides, but recommendations can well be adopted for sucking insect pests for good agricultural practices in chickpea.

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Acknowledgments

The senior author is grateful to Indian Council of Agricultural Research (ICAR) for providing Junior Research Fellowship. Due acknowledgement is expressed to the authorities of Divisions of Entomology, Seed Science and Technology, Microbiology, Plant Pathology and Genetics, and Central Library of IARI for requisite infrastructure and facilities for undertaking this study. This work was also supported by the National Fellow ICAR project.

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Authors and Affiliations

  1. Division of Entomology, ICAR - Indian Agricultural Research Institute, Pusa, New Delhi, 110 012, India

    Shivaji Hausrao Thube & Gagan Kumar Mahapatro

  2. Division of Seed Science and Technology, ICAR - Indian Agricultural Research Institute, Pusa, New Delhi, 110 012, India

    M. B. Arun Kumar

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  1. Shivaji Hausrao Thube
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  2. Gagan Kumar Mahapatro
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  3. M. B. Arun Kumar
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Correspondence to Gagan Kumar Mahapatro.

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Thube, S.H., Mahapatro, G.K. & Arun Kumar, M.B. In vitro Evaluation of Insecticides, Bio-Fungicide and Bio-Fertilizer for Strategic and Eco-Friendly Combinatorial Seed Treatments in Chickpea. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 645–654 (2018). https://doi.org/10.1007/s40011-016-0794-z

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