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Lower Frequency and Diversity of Antibiotic-Producing Fluorescent Pseudomonads in Rhizosphere of Indian Rapeseed–Mustard (Brassica juncea L. Czern.)

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Fluorescent pseudomonads are one of the most important microbial communities which play a key role in rhizosphere to enhance plant growth-promotion and protection. The diverse groups of antibiotics viz. 2,4-diacetylphloroglucinol (DAPG), phenazine-1-carboxylic acid (PCA) and pyoluteorin (PLT) are produced by fluorescent pseudomonads inhibiting growth of fungal pathogens which results in health upliftment of plants. The present study, discusses about frequency and diversity of 138 antibiotic-producing fluorescent pseudomonads isolated from eight genotypes of rapeseed mustard rhizosphere (Brassica juncea L. Czern.). The plant growth promoting traits and antibiotics (DAPG, PCA and PLT) production of isolates were examined by using polymerase chain reaction (PCR), thin layer chromatography (TLC) and dot blot-hybridization. Among 138 isolates, 47, 25 and 9 % of isolates were positive in indole production, phosphate solubilization and antagonism potential against Sclerotinia sclerotiorum (causal agent of white mold disease in rapeseed mustard), respectively. PCR amplifications showed that none of the isolates had phlD (DAPG) and phzC (PCA) genes, but four isolates (UKA-2, UKA-8, UKA-11, UKA-66) had pltB (PLT) gene, which was further confirmed by TLC and DNA dot-blot hybridization. BOX profiles of pltB positive isolates were distinct, showing unique genetic diversity in the small population. The four pltB positive fluorescent pseudomonad isolates could be used as promising bio-control and plant growth-promoting inoculants for Indian rapeseed mustard.

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Abbreviations

FP:

Fluorescent pseudomonads

IRM:

Indian rapeseed mustard

DAPG:

2,4-Diacetyl phloroglucinol

PCA:

Phenazine-1-carboxylic acid

PLT:

Pyoluteorin

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Acknowledgments

The first author thanks to Indian Council of Agricultural Research (ICAR) and Indian Agricultural Research Institute, New Delhi, India for providing financial assistance in the form of Junior Research Fellowship (JRF) and laboratory facility of conducting research work, respectively.

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Authors and Affiliations

  1. Microbiology Laboratory, ICAR-National Rice Research Institute, Cuttack, Odisha, India

    Upendra Kumar, P. Panneerselvam & Avishek Banik

  2. Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    K. Annapurna

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  1. Upendra Kumar
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  2. P. Panneerselvam
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Correspondence to Upendra Kumar.

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Kumar, U., Panneerselvam, P., Banik, A. et al. Lower Frequency and Diversity of Antibiotic-Producing Fluorescent Pseudomonads in Rhizosphere of Indian Rapeseed–Mustard (Brassica juncea L. Czern.). Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 579–586 (2018). https://doi.org/10.1007/s40011-016-0792-1

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  • DOI: https://doi.org/10.1007/s40011-016-0792-1

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