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Trachyspermum ammi L. (Carom) Oil Induces Alterations in SOD-3, GST-4 Expression and Prolongs Lifespan in Caenorhabditis elegans

  • Laxmi Rathor
  • Aakanksha Pant
  • Abhishek Nagar
  • Sudeep Tandon
  • Shalini Trivedi
  • Rakesh PandeyEmail author
Research Article

Abstract

The use of herbs in the form of dietary supplements and medicine is fascinating the world, owing to their safety and protective effects against oxidative stress. The elevation in oxidative stress promotes aging and age related disorders. To this end, the authors evaluated in vivo antioxidant activity and anti-aging potential of Trachyspermum ammi L. (TA) oil in Caenorhabditis elegans for the first time. This medicinally important herb is commonly known as carom seed, ajwain, Bishop’s weed and traditionally used as spice in food preparations in India and other parts of world. The authors evaluated three doses of TA oil (10, 100 and 1000 ppm) and 10 ppm dose demonstrated maximal lifespan extension (12.45 %, P ≤ 0.0001) in C. elegans. The 10 ppm dose of TA oil demonstrated in vivo antioxidant activity as it was able to extend lifespan in oxidative stress sensitive mev-1 mutant by 9.53 % (P < 0.0001). Furthermore, TA oil treatment elevated expression of SOD-3 and GST-4 and conferred oxidative stress resistance in worms. This study broadens current perspectives concerning antiaging potential of TA oil. The present study has implications in the development of natural antioxidant based therapy for treating age related disorders which can be subjected to future investigations.

Keywords

Trachyspermum ammi Aging Stress Superoxide dismutase Glutathione-S-transferase 

Abbreviations

TA

Trachyspermum ammi

C. elegans

Caenorhabditis elegans

SOD-3

Superoxide dismutase-3

GST-4

Glutothione-S-transferase-4

Notes

Acknowledgments

The authors are highly grateful to the Caenorhabditis Genetics Centre (CGC), Minneapolis, MN, USA, which is funded by the NIH, National Centre for Research Resources (USA), for providing C. elegans strains. The authors are also thankful to the Director, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India for his valuable support.

Compliance with Ethical Standards

Conflict of interest

Authors declare that they have no conflict of interest.

Human or Animal Studies

This article contain studies on Caenorhabditis elegans model system.

Supplementary material

40011_2016_710_MOESM1_ESM.doc (2.1 mb)
Supplementary material 1 (DOC 2194 kb)

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Copyright information

© The National Academy of Sciences, India 2016

Authors and Affiliations

  • Laxmi Rathor
    • 1
  • Aakanksha Pant
    • 1
  • Abhishek Nagar
    • 2
  • Sudeep Tandon
    • 2
  • Shalini Trivedi
    • 1
  • Rakesh Pandey
    • 1
    Email author
  1. 1.Microbial Technology and Nematology DepartmentCSIR-Central Institute of Medicinal and Aromatic PlantsLucknowIndia
  2. 2.Chemical Processing DepartmentCSIR-Central Institute of Medicinal and Aromatic PlantsLucknowIndia

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