Abstract
Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity (He) and observed heterozygosity (Ho) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies.
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The authors acknowledge the gracious funding provided by the Department of Biotechnology, Government of India. The authors are also thankful to Dr. R. K. Pachauri, Director General, TERI, for his kind support.
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Islam, M.A., Sinha, P., Sharma, S.S. et al. Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 727–732 (2017). https://doi.org/10.1007/s40011-015-0646-2
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DOI: https://doi.org/10.1007/s40011-015-0646-2