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Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Municipal sewage triggers a stress prone environment to accumulate polyhydroxyalkanoates (PHAs) in the cytosol of bacteria. In view of that, different Bacillus species were isolated from municipal sewage and screened for evaluating their efficacy of PHA production. Growth parameters such as temperature, pH, glucose concentration and carbon nitrogen combinations were optimized with respect to higher biomass production as it is analogous to PHA accumulation. Under optimized conditions, the Bacillus species produced 3.09 g/L of PHAs which was estimated as a higher yield in comparison to other similar strains. Fourier transform infrared spectroscopic analysis of the extracted polyhydroxybutyrate confirmed the distinct peak corresponding to C=O group, whereas proton nuclear magnetic resonance (1H NMR) and differential scanning colorimetric analysis exhibited detailed insight of its chemical structure and properties by reflecting monomeric unit. The high yielding bacterial isolate was identified by 16S rDNA sequencing and the sequence was confirmed as Bacillus subtilis with an accession no. KP172548 after submission to NCBI data base. The potential bacterium may be further exploited for cost effective and mass scale production of biopolymer.

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Acknowledgments

The authors are thankful to Dr. B. B. Mishra (HOD, Microbiology), Dr. N. Sahoo, Dr. S. Acharya (In-charge of Central laboratory, OUAT) and Dr. G. S. Acharya for providing laboratory facilities during the period of study. They are also thankful to S. Basu and H. R. Dash of NIT, Rourkela, India for their support during the study. The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Department of Microbiology, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, 751003, India

    S. Mohapatra, P. R. Mohanta & D. P. Samantaray

  2. National Institute of Abiotic Stress Management, Malegaon, Baramati, Pune, Maharashtra, 413115, India

    B. Sarkar & A. Daware

  3. Department of Chemistry, Sheila Dhar Institute of Soil Science, University of Allahabad, Allahabad, Uttar Pradesh, 211002, India

    C. Kumar

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  1. S. Mohapatra
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Correspondence to B. Sarkar or D. P. Samantaray.

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Mohapatra, S., Mohanta, P.R., Sarkar, B. et al. Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 459–466 (2017). https://doi.org/10.1007/s40011-015-0626-6

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  • DOI: https://doi.org/10.1007/s40011-015-0626-6

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