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In Vitro Antibacterial Activity of Ethno Medicinally Used Lichens Against Three Wound Infecting Genera of Enterobacteriaceae

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Lichens represent mutualistic associations between a fungus and one or more extracellularly located photobiont (algae or cyanobacteria). Hypotrachyna cirrhata (Fr.) Divakar et al. and Flavoparmelia caperata (L.) Hale are used ethno medicinally against wound infections, bite and burn, by the folks of Sikkim and Tamil Nadu, India, respectively. Present study was focused on the efficacy of aforementioned lichen extracts against the members of family Enterobacteriaceae. Enterobacteriaceae a heterogeneous family, comprised of coliform bacteria. Genera comprised in this family have evolved drug resistance mechanism against first line clinical drugs. Clinical and Laboratory Standard Institute recommended antimicrobial susceptibility test which was performed to test the susceptibility of pathogens. Acetone extract of H. cirrhata (Fr.) Divakar et al. and F. caperata (L.) Hale was found to be effective against Vibrio cholerae, Klebsiella pneumoniae and Salmonella typhimurium with MIC value 2.48 ± 0.02, 2.24 ± 0.02 and 2.28 ± 0.03 mg/ml; and 3.29 ± 0.06, 4.40 ± 0.05 and 4.96 ± 0.06 mg/ml, respectively. The aforementioned lichens showed promising antibacterial activity and provided new tools to develop novel drugs.

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Acknowledgments

Thanks are due to the Head, Department of Botany, University of Allahabad for laboratory facilities, Dr. D.K. Upreti, Deputy Director, National Botanical Research Institute (NBRI), Lucknow, for identification of lichens, Dr. G.P. Sinha, Head, Botanical Survey of India, Central Regional Centre, Allahabad for accepting the voucher specimens of Lichens and University Grant Commission (UGC) New Delhi, India, for financial assistance.

Conflict of interest

The authors have no conflict of interest.

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Ethical approval was not required in the present study.

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Authors and Affiliations

  1. Biological Product Lab, Department of Botany, University of Allahabad, Allahabad, 211002, Uttar Pradesh, India

    Ashutosh Pathak, Shashi Kant Shukla, Anand Pandey, Rohit K. Mishra, Rajesh Kumar & Anupam Dikshit

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  1. Ashutosh Pathak
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  2. Shashi Kant Shukla
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  3. Anand Pandey
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  4. Rohit K. Mishra
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  5. Rajesh Kumar
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  6. Anupam Dikshit
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Correspondence to Anupam Dikshit.

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Pathak, A., Shukla, S.K., Pandey, A. et al. In Vitro Antibacterial Activity of Ethno Medicinally Used Lichens Against Three Wound Infecting Genera of Enterobacteriaceae. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 863–868 (2016). https://doi.org/10.1007/s40011-015-0540-y

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  • DOI: https://doi.org/10.1007/s40011-015-0540-y

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