Expression Profile of Leptin and Aromatase-P450 Genes and Association with Sperm Motility in Fresh Bovine Ejaculates
- 102 Downloads
Aromatase-P450 is an enzyme responsible for estrogen biosynthesis. It plays a major role in normal spermatogenesis and controls the sexual development and neuro-endocrine functions. Leptin, a 167 amino acid long protein-hormone, is the product of obese gene secreted by adipocytes. In the present study the expression of Leptin and Aromatase-P450 in the fresh ejaculates, using SYBR Green chemistry in real time PCR, from a total of 10 bulls- 4 buffalo and 6 crossbred cattle bulls were studied. The experimental animals were further categorized into acceptable (individual motility >50 %, post thaw motility >45 % in cattle and >40 % in buffalo) or poor semen samples (individual motility <50 %, post thaw motility <45 % in cattle and < 40 % in buffalo). The expression of Aromatase-P450 was found to be higher in acceptable semen ejaculates whereas for Leptin gene it was higher in poor quality semen for both the species.Sperm mitochondrial activity index (SMAI) was performed to determine the association between the activity of sperm mitochondria, individual motility with the expression of both the genes. Samples with high mass motility were showing high depositions of NBT dye and high expression of Aromatase-P450 gene but low expression of Leptin gene as compared to low mass motility. In conclusion, higher expression of aromatase and leptin genes in fresh ejaculates is directly and inversely proportional respectively, to higher sperm motility in both cattle and buffalo bulls.
KeywordsLeptin AromataseP450 Expression Sperm motility Real time polymerase chain reaction
The present work has been funded by the Rashtriya Krishi Vikash Yojna (RKVY Scheme), Department of Agriculture and Cooperation, Ministry of Agriculture, Government of India.
Conflict of interest
There is no conflict of interest among the authors
- 2.Hess RA, Bunick D, Lee KH, Bahr J, Taylor JA, Korach KS (1997) A role for estrogens in the male reproductive tract. Nature 12:390–509Google Scholar
- 9.Jorsaraei SGA, Shibahara H, Ayustawati, Hirano Y, Suzuki T, Marzony ET, Zainalzadeh M, Suzuki M (2010) The Leptin concentrations in seminal plasma of men and its relationship to semen parameters. Iran J Reprod Med 8(3):95–100Google Scholar
- 11.Bhat GK, Seaq TL, Olatinwo MO, Simorangkir D, Ford GD, Ford BD, Mann DR (2005) Influence of a leptin deficiency on testicular morphology, germ cell apoptosis and expression levels of apoptosis-related genes in the mouse. J Androl 22:1–30Google Scholar
- 13.Mukhopadhyay CS, Verma A, Joshi BK, Chakravarty AK (2008) Association of sperm mitochondrial activity index with gross seminal parameters and fertility in crossbred bulls. Indian J Anim Res 42(4):282–284Google Scholar
- 16.Kunova S, Hleba L, Hascik P, Cubon J, Kacaniova M (2011) Determination of leptin expression in beef cattle blood samples used by RTQ PCR. J Microbiol, Biotechnol Food Sci 1(1):21–38Google Scholar
- 18.Benjamin BR, Sahni KL, Mohan G (1989) Applicability of NBT reductase test for evaluating the extended semen of cattle and buffaloes. Indian J Anim Sci 59(1):99–100Google Scholar
- 19.Gopalkrishnan K, Hinduja IN, Kumar TC (1991) Assessment of mitochondrial activity of human spermatozoa: motility/viability in fertile/infertile men. Mol Androl 3:243–250Google Scholar