Expression Profile of Leptin and Aromatase-P450 Genes and Association with Sperm Motility in Fresh Bovine Ejaculates

  • Jasdeep Kaur Dhanoa
  • C. S. Mukhopadhyay
  • Jaspreet Singh Arora
  • Simarjeet Kaur
Research Article

Abstract

Aromatase-P450 is an enzyme responsible for estrogen biosynthesis. It plays a major role in normal spermatogenesis and controls the sexual development and neuro-endocrine functions. Leptin, a 167 amino acid long protein-hormone, is the product of obese gene secreted by adipocytes. In the present study the expression of Leptin and Aromatase-P450 in the fresh ejaculates, using SYBR Green chemistry in real time PCR, from a total of 10 bulls- 4 buffalo and 6 crossbred cattle bulls were studied. The experimental animals were further categorized into acceptable (individual motility >50 %, post thaw motility >45 % in cattle and >40 % in buffalo) or poor semen samples (individual motility <50 %, post thaw motility <45 % in cattle and < 40 % in buffalo). The expression of Aromatase-P450 was found to be higher in acceptable semen ejaculates whereas for Leptin gene it was higher in poor quality semen for both the species.Sperm mitochondrial activity index (SMAI) was performed to determine the association between the activity of sperm mitochondria, individual motility with the expression of both the genes. Samples with high mass motility were showing high depositions of NBT dye and high expression of Aromatase-P450 gene but low expression of Leptin gene as compared to low mass motility. In conclusion, higher expression of aromatase and leptin genes in fresh ejaculates is directly and inversely proportional respectively, to higher sperm motility in both cattle and buffalo bulls.

Keywords

Leptin AromataseP450 Expression Sperm motility Real time polymerase chain reaction 

Notes

Acknowledgments

The present work has been funded by the Rashtriya Krishi Vikash Yojna (RKVY Scheme), Department of Agriculture and Cooperation, Ministry of Agriculture, Government of India.

Conflict of interest

There is no conflict of interest among the authors

References

  1. 1.
    Tiwari A, Sharma MK, Kumar OS (2008) Expression of cytochrome P450 aromatase transcripts in buffalo (Bubalus bubalis)-ejaculated spermatozoa and its relationship with sperm motility. Domest Anim Endocrinol 34:238–249CrossRefPubMedGoogle Scholar
  2. 2.
    Hess RA, Bunick D, Lee KH, Bahr J, Taylor JA, Korach KS (1997) A role for estrogens in the male reproductive tract. Nature 12:390–509Google Scholar
  3. 3.
    Robertson KM, O’Donnell L, Jones ME, Meacham SJ, Boon WC, Fisher CR (1999) Impairment of spermatogenesis in mice lacking a functional aromatase (Cyp 19) gene. Nat Acad Sci USA 96:7986–7991CrossRefGoogle Scholar
  4. 4.
    Abavisani A, Baghbanzadeh A, Shayan P, Tajik P, Dehghani H, Mirtorabi M (2009) Leptin mRNA expresses in the bull reproductive organ. Vet Res Commun 33:823–830CrossRefPubMedGoogle Scholar
  5. 5.
    Glander HJ, Lammert A, Paasch U, Glasow A, Kratzsch J (2002) Leptin exist in tubuli seminiferi and in seminal plasma. Andrologia 34:227–233CrossRefPubMedGoogle Scholar
  6. 6.
    Lackey BR, Gray SL, Henricks DM (2002) Measurement of leptin and insulin-like growth factor-I in seminal plasma from different species. Physiol Res 51:309–311PubMedGoogle Scholar
  7. 7.
    Jope T, Lammert A, Kratzsch J, Paasch U, Glander HJ (2003) Leptin and leptin receptor in human seminal plasma and in human spermatozoa. Int J Androl 26:335–341CrossRefPubMedGoogle Scholar
  8. 8.
    Aquila S, Rago V, Guido C, Zupo S, Casaburi I, Carpino A (2008) Leptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival. Reproduction 136:23–32CrossRefPubMedGoogle Scholar
  9. 9.
    Jorsaraei SGA, Shibahara H, Ayustawati, Hirano Y, Suzuki T, Marzony ET, Zainalzadeh M, Suzuki M (2010) The Leptin concentrations in seminal plasma of men and its relationship to semen parameters. Iran J Reprod Med 8(3):95–100Google Scholar
  10. 10.
    Barash IA, Cheung CC, Weigle DS, Ren H, Kabigting EB, Kuijper JL, Clifton DK, Steiner RA (1996) Leptin is a metabolic signal to the reproductive system. Endocrinology 137:3144–3314PubMedGoogle Scholar
  11. 11.
    Bhat GK, Seaq TL, Olatinwo MO, Simorangkir D, Ford GD, Ford BD, Mann DR (2005) Influence of a leptin deficiency on testicular morphology, germ cell apoptosis and expression levels of apoptosis-related genes in the mouse. J Androl 22:1–30Google Scholar
  12. 12.
    Wrann CD, Eguchi J, Bozec A, Xu Z, Mikkelsen T, Gimble J, Nave H, Wagner EF, Ong S-E, Rosen ED (2012) FOSL2 promotes leptin gene expression in human and mouse adipocytes. J Clin Invest 122(3):1010–1021CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Mukhopadhyay CS, Verma A, Joshi BK, Chakravarty AK (2008) Association of sperm mitochondrial activity index with gross seminal parameters and fertility in crossbred bulls. Indian J Anim Res 42(4):282–284Google Scholar
  14. 14.
    Toda K, Takeda K, Okada T, Akira S, Saibara T, Kaname T, Yamamura K, Onishi S, Shizuta Y (2001) Targeted disruption of the aromatase P450 gene (Cyp19) in mice and their ovarian and uterine responses to 17-oestradiol. J Endocrinol 170:99–111CrossRefPubMedGoogle Scholar
  15. 15.
    Jones ME, Boon WC, Poietto J, Simpson ER (2006) Of mice and men: the evolving phenotype of aromatase deficiency. Trends Endocrinol Metab 17:55–64CrossRefPubMedGoogle Scholar
  16. 16.
    Kunova S, Hleba L, Hascik P, Cubon J, Kacaniova M (2011) Determination of leptin expression in beef cattle blood samples used by RTQ PCR. J Microbiol, Biotechnol Food Sci 1(1):21–38Google Scholar
  17. 17.
    Palmer NO, Bakos HW, Fullston T, Lane M (2012) Impact of obesity on male fertility, sperm function and molecular composition. Spermatogenesis 2(4):253–263CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Benjamin BR, Sahni KL, Mohan G (1989) Applicability of NBT reductase test for evaluating the extended semen of cattle and buffaloes. Indian J Anim Sci 59(1):99–100Google Scholar
  19. 19.
    Gopalkrishnan K, Hinduja IN, Kumar TC (1991) Assessment of mitochondrial activity of human spermatozoa: motility/viability in fertile/infertile men. Mol Androl 3:243–250Google Scholar
  20. 20.
    Hrudka F (1979) Cytochemistry of oxidoreductases in spermatozoa: the technique revisited. Andrologia 11(5):337–353CrossRefPubMedGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2015

Authors and Affiliations

  • Jasdeep Kaur Dhanoa
    • 1
  • C. S. Mukhopadhyay
    • 1
  • Jaspreet Singh Arora
    • 1
  • Simarjeet Kaur
    • 2
  1. 1.School of Animal BiotechnologyGuru Angad Dev Veterinary and Animal Sciences University (GADVASU)LudhianaIndia
  2. 2.Department of Animal Genetics and BreedingGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia

Personalised recommendations