Abstract
Application of municipal solid waste and sewage sludge to supplement soil is an age-old agronomic practice due to its rich organic contents. These soil-supplements also contain significant amount of non-essential heavy metals, posing threat to crop yield and human health. The present study aims to compare the phytotoxicity of the heavy metals and potential dietary toxicity of some selected vegetables grown in soil supplemented with municipal solid waste and sewage sludge. To assess the phytotoxicity of the supplements, plants were grown in three different sets; (1) Hoagland’s nutrient media [as control] (2) municipal solid waste and (3) sewage sludge soil mix. To study the phytotoxic markers, photosynthetic pigments, proline, protein content and antioxidant enzyme activities were measured and metal content of the vegetables were measured to estimate the potential dietary toxicity. It was observed from the study that vegetables grown in supplemented soils showed reduced chlorophyll and protein content while carotenoid, proline and antioxidative enzymes showed enhanced activity. In both the supplemented soil, it was observed that cadmium was present above the maximum allowable limits, amount of lead was marginal and that of chromium was below the level. It was observed that the two leafy vegetables, i.e., Lagenaria sp. and Cucurbita sp. accumulated more lead in their aerial parts, Lagenaria grown in sewage sludge supplemented soil also accumulated significant amount of cadmium in the aerial parts. Edible parts of Raphanus and maize grains also accumulated considerable amount of cadmium, which were above the value of daily tolerable limits.
Similar content being viewed by others
References
Dulac N (2001) The organic waste flow in integrated sustainable waste management. Tools for Decision-makers Experiences from the Urban Waste Expertise Programme (1995–2001). In: Scheinberg A (ed) Waste Advisors on urban environment and development, Niewhaven 201,2801CW Gouda, The Netherlands
Das S, Bhattacharya BK (2013) Municipal solid waste characteristics and management in Kolkata, India. Int J Emerg Technol Adv Eng 3(2):147–152
Alloway BJ, Jackson AP (1991) The behaviour of heavy metals in sewage sludge-amended soil. Sci Total Environ 100:151–176
Ma OY, Traina SJ, Logan TJ (1994) Effect of aqueous Al, Cd, Fe(II), Ni and Zn on Pb immobilization by hydroxyapatite. Environ Sci Technol 28(7):1219–1228
Eslami A, Kaniki GRJ, Nurani M, Mehrasbi M, Peyda M, Azimi R (2007) Heavy metal in edible green vegetable grown along the sites of the Zanja road river in Zanjan, Iran. J Biol Sci 7(6):943–948
Bahemuka TE, Mubofu EB (1991) Heavy metals in edible green vegetables grown along the sites of the Sinza and Msimbazi river in Dar es salaam, Tanzania. Food Chem 66:63–66
Mohamed HH, Ali Khairia M, Al-Qahtani (2012) Assessment of some heavy metals in vegetables, cereals and fruits in Saudi Arabian markets. Egypt J Aquat Res 38(1):31–37
Turkdogan MK, Kilicel F, Kara K, Tuncer I, Uygan I (2003) Heavy metal in soil vegetable and fruits in the endemic upper gastrointestinal cancer region of Turkey. Environ Toxicol Pharmacol 13(3):175–179
Damek-Poprawa M, Sawicka-Kapusta K (2003) Damage to liver, kidney and testis with reference to burden of heavy metals in yellow-necked mice from areas around steel works and zinc smelters in Poland. Toxicology 186(1–2):1–10
Arora M, Kiran B, Rani S, Rani A, Kaur B, Mittal N (2008) Heavy metal accumulation in vegetables irrigated with water from different sources. Food Chem 111:811–815
WHO (1992) DEFRA (Dept. Of Environ. Food & Rural affairs) & Environmental Agency. Contaminated lead exposure assessment model CCLEA): Technical Basis and algorithms. Bristol UK 2002
Jarup L (2003) Hazards of heavy metal contaminations. Br Med Bull 68:167–182
Methane to Markets. Assessment report on Dhapa disposal site, Kolkata, India (2010) http//www.Globalmethane.org/data/1128_dhapa.assessment.report.4-27-10.pdf Accessed 26 Dec 2013
APHA (1992) Standard methods for the examination of water and wastewater, 14th edn. APHA-AWWA-WPCF, Washington D.C 1020
Arnon DI (1949) Copper enzyme in isolated chloroplasts: polyphenol oxidase in Beta vulgaris. Plant Physiol 24(1):1–15
Daevis BH (1976) Carotenoids. In: Goodwin TW (ed) Chemistry and biochemistry of plant pigments, vol 2. Academic Press Inc, London, pp 38–165
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin-phenol reagent. J Biol Chem 193:265–275
Bates LS, Waldern RP, Teare JD (1973) Rapid determination of proline for water stress studies. Plant Soil 39:205–207
Beuchamp C, Fridovich I (1971) Superoxide dismutase, improved assays and an assay applicable to acrylamide gels. Anal Biochem 44(1):276–287
Chat field JM, Dalton DA (1993) Ascorbate peroxide from soybean root nodules. Plant Physiol 103:661–662
Wen-Chi H, Liang HJ, Wang CC, Liu DZ (2004) Detection of glutathione reductase after electrophoresis on native or sodium dodecyl sulphate polyacrylamide gels. Electrophoresis 25:2926–2931
Rattan RK, Dutta SP, Chhonkar PK, Suribabu K, Singh AK (2005) Long term impact of irrigation with sewage effluents on heavy metal contents in soil, crops and ground water—a case study. Agric Ecosyst Environ 109:310–322
Wang X, Sato T, Xing B, Tao S (2005) Health risks of heavy metal to the general public in Tianjin, China via consumption of vegetable and fish. Sci Total Environ 350:28–37
Asada K (1992) Ascorbate peroxidise—a hydrogen peroxide scavenging enz. in plants. Physiol Plant 85:235–241
Codex Alimentarius Commission (2011) Joint WHO/FAO food standard programme. Codex Committee on contaminants in foods. Fifth Session. The Hague. The Netherlands, 21–25 March, 2011. ftp://ftp.fao.org/codex/meetings/CCCF/cccf5/cf05_INF.pdf
Alloway BJ (1995) Soil processes and the behaviour of metals. In: Alloway BJ (ed) Heavy metals in soils, 2nd edn. Blackie Academic and Professional, London
McBride M, Sauve S, Hendershot W (1997) Solubility control of Cu, Zn, Cd and Pb in contaminated soils. Eur J Soil Sci 48:337–346
Kabata-Pendias A, Pendias H (2001) Trace elements in soils and plants, 3rd edn. CRC Press, Boca Raton
European Union (2002) Heavy metals in wastes, European Commission on Environment, http://eu.europa.eu/environment/waste/studies/pdf/heavymetalsreport.pdf
Cui S, Zhou Q, Chao L (2007) Potential hyperaccumulation of Pb, Zn, Cu and Cd in endurant plant distributed in an old smeltery, northeast China. Environ Geol 51:1043–1048
Baker AJM, Brooks RR (1989) Terrestrial higher plants which hyperaccumulate metallic elements-a review of their distribution, ecology and phytochemistry. Biorecovery 1:81–126
Topcouglu B, Önal MK (2007) Heavy metal accumulation in the eggplant (Solanum melongena) grown in MSW compost applied soil utilization of diversity in land use systems. Sustainable and organic approaches to meet human needs Tropentag 2007, October 9–11, Witzenhausen, Germany, 2007
Nayek S, Gupta S, Saha R (2010) Effects of metal stress on biochemical response of some aquatic macrophytes growing along an industrial waste discharge channel. J Plant Interact 5(2):91–99
Ahmad P, Sharma S, Srivastava PS (2007) In vitro selection of NaHCO3 tolerant cultivars of Morus alba (Local & Sujanpuri) in response to morphological & biochemical parameters. Hortic Sci (Prague) 34:114–122
Hou W, Chen X, Song G, Wang Q, Chang CC (2007) Effects of copper and cadmium on heavy metal polluted waterbody restoration by duckweed (Lemna minor). Plant Physiol Biochem 45:62–69
Koca H, Bor M, Ozdemir F, Turkan I (2007) The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environ Exp Bot 60:344–351
Matysik J, Alia BB, Mohanty P (2002) Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr Sci 82:525–532
Hamid N, Buchari N, Jawaid F (2010) Physiological responses of Phaseolus vulgaris to different lead concentration. Pak J Bot 42(1):239–246
Singh S, Sinha S (2005) Accumulation of metal and its effects in Brassica juncea L. Czern. (var. Rohini) grown on various amendments of tannery wastes. Ecotoxicol Environ Saf 62:118–127
Hassan MJ, Wang Z, Zhang G (2005) Sulfur alleviates growth inhibition and oxidative stress caused by cadmium toxicity in rice. J Plant Nutr 28:1785–1800
Somshekaraiah BV, Padmaja K, Prassad ARK (1992) Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): involvement of lipid peroxides in chlorophyll degradation. Physiol Plant 85:85–89
Vitoria AP, Lea PJ, Azevedo RA (2001) Antioxidant enzymes responses to cadmium in radish tissues. Phytochemistry 57:710–715
Awasthi SK (2000) Prevention of food adulteration Act No. 37 of, 1954. Central and State Rules as Amended for 1999. Ashoka Law House, New Delhi
Acknowledgments
The authors would like to acknowledge the Department of Botany, University of Calcutta for the financial assistance, R.K is grateful to A. Bhattacharya and S. Paul for their help in preparing the manuscript.
Conflict of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pal, R., Kundu, R. Risk Assessment of Some Selected Vegetables Grown in Metal Contaminated Soil Supplements. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 585–593 (2016). https://doi.org/10.1007/s40011-015-0491-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40011-015-0491-3