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Effect of Preconceptional Exposure of Triazophos Formulation on Fertility and Reproductive Performance of Female Wistar Rats, Rattus norvegicus

Abstract

Present study was undertaken to investigate the effects of triazophos (TZ) preconceptional exposure of 30 days at three different doses on fertility and reproductive performance of female rats. Non-significant change in reproductive indices like gestational length and pup viability was observed in all TZ treated groups. Subtle reduction in litter size and significantly altered male:female sex ratio was observed in all the TZ treated groups. Clinical signs such as hyperactivity, impaired gait, forelimb and hindlimb paralysis and increased head elevations were observed in pups born to 1/10th of LD50 TZ treated female rats. Reduced body weight of the pups was observed in 1/10th and 1/20th of LD50 TZ treated group during 30 days postnatal development. Developmental milestones were not significantly altered except fur development which was significantly delayed in pups of 1/10th and 1/20th treated groups. The results suggest that TZ preconceptional exposure may affect reproductive performance. Further investigation with higher doses and skeleton study is suggested for developmental parameters in the offsprings.

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References

  1. Jaga K, Dharmani C (2003) Sources of exposure to and public health implications of organophosphate pesticides. Am J Public Health 14(3):171–185

    Google Scholar 

  2. Casida JE, Quistad GB (2004) Organophosphate toxicology: safety aspects of nonacetylcholinesterase secondary targets. Chem Res Toxicol 17:983–998

    CAS  Article  PubMed  Google Scholar 

  3. Leibson T, Lifshutz M (2008) Organophosphate and carbamate poisoning: review of the current literature and summary of clinical and laboratory experience in southern Israel. Isr Med Assoc J 10:767–770

    PubMed  Google Scholar 

  4. Rani S, Madan VK, Kathpal TS (2001) Persistence and dissipation behavior of triazophos in canal water under Indian climatic conditions. Ecotoxicol Environ Saf 50(1):82–84

    CAS  Article  PubMed  Google Scholar 

  5. Li W, Qiu S, Wu Y (2008) Triazophos residues and dissipation rates in wheat crops and soil. Ecotoxicol Environ Saf 69(2):312–316

    CAS  Article  PubMed  Google Scholar 

  6. Bretveld R, Brouwers M, Ebisch I, Roeleveld N (2007) Influence of pesticides on male fertility. Scand J Work Environ Health 33(1):13–28

    CAS  Article  PubMed  Google Scholar 

  7. Arbuckle TE, Lin Z, Mery LS (2001) An exploratory analysis of the effect of pesticides exposure on the risk of spontaneous abortion in an Ontario farm population. Environ Health Perspect 109(8):851–857

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  8. Chia S, Shi L (2002) Review of recent epidemiological studies on paternal occupational and birth defects. Occup Environ Med 59:149–155

    PubMed Central  Article  PubMed  Google Scholar 

  9. Lacasaña M, Vázquez-Grameix H, Borja-Aborto V, Blanco-Muñòz J, Romieu I, Aguilar-Garduño C, García A (2006) Maternal and paternal occupational exposure to agricultural work and the risk of anencephaly. Occup Environ Med 63:649–656

    PubMed Central  Article  PubMed  Google Scholar 

  10. Garry VF, Schreinemachers D, Harkins ME, Griffith J (1996) Pesticide appliers, biocides, and birth defects in rural Minnesota. Environ Health Perspect 104:394–399

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  11. Pastore LM, Hertz-Picciotto I, Beaumont JJ (1997) Risk of stillbirth from occupational and residential exposures. Occup Environ Med 54:511–518

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  12. Eskenazi B, Rosas LG, Marks AR, Bradman A, Harley K, Holland N, Johnson C, Fenster L, Barr DB (2008) Pesticide toxicity and the developing brain. Basic Clin Pharmacol Toxicol 102:228–236

    CAS  Article  PubMed  Google Scholar 

  13. Rosas LG, Eskenazi B (2008) Pesticides and child neurodevelopment. Curr Opin Pediatr 20:191–197

    Article  PubMed  Google Scholar 

  14. Nurulain SM, Shafiullah M (2012) Teratogenicity and embryotoxicity of organophosphorus compounds in animal models—a short review. Mil Med Sci Lett (Voj. Zdrav. Listy) 81(1):16–26

    Google Scholar 

  15. Sharma D, Sangha GK, Khera KS (2014) Triazophos induced oxidative stress in female albino rats. Int J Adv Res 2(1):746–754

    CAS  Google Scholar 

  16. Sharma D, Sangha GK (2014) Triazophos induced oxidative stress and histomorphological changes in liver and kidney of female albino rats. Pestic Biochem Physiol 110:71–80

    CAS  Article  PubMed  Google Scholar 

  17. Turner KJ, Barlow NJ, Struve MF, Wallace DG, Gaido KW, Dorman DC, Foster PMD (2002) Effects of in utero exposure to the organophosphate insecticide fenitrothion on androgen-dependent reproductive development in the Crl:CD(SD)BR rat. Toxicol Sci 68:174–183

    CAS  Article  PubMed  Google Scholar 

  18. Eskenazi B, Harley K, Bradman A, Weltzien E, Jewell NP, Barr DB, Furlong CE, Holland NT (2004) Association of in utero organophosphate pesticide exposure and fetal growth and length of gestation in an agricultural population. Environ Health Perspect 112(10):1116–1124

    PubMed Central  CAS  Article  PubMed  Google Scholar 

  19. Sortur SM, Kaliwal BB (1999) Effect of methyl parathion formulation on estrous cycle and reproductive performance in albino rats. Indian J Exp Biol l37:176–178

    Google Scholar 

  20. Ngoula F, Watcho P, Kenfack A, Manga JN, Defang HF, Pierre K, Joseph T (2014) Effect of dimethoate (an organophosphate insecticide) on the reproductive system and fertility of adult male rat. Am J Pharmacol Toxicol 9(1):75–83

    Article  Google Scholar 

  21. Breslin WJ, Liberackj AB, Dittenber DA, Quast JF (1996) Evaluation of the developmental and reproductive toxicity of chlorpyrifos in the rat. Fundam Appl Toxicol 29(1):19–130

    Google Scholar 

  22. Shalaby MA, Abo-El-Sooud K, Hamoda AA (2013) Assessment of toxicity of chlorpyrifos insecticide on fetuses and suckling pups of rats. Insight Ecol 2:1–7. doi:10.5567/ECOLOGY-IK.2013.1.7

    Article  Google Scholar 

  23. Sallmén M, Liesivuori J, Taskinen H, Lindbohm ML, Anttila A, Alto L, Hemminki K (2003) Time to pregnancy among wives of Finnish greenhouse workers. Scand J Work Environ Health 29(2):85–93

    Article  PubMed  Google Scholar 

  24. Garry VF, Harkins ME, Erickson LL, Long-Simpson LK, Holland SE, Burroughs BL (2002) Births defects, season of conception, and sex of children born to pesticide applicators living in the Red River Valley of Minnesota, USA. Environ Health Perspect 110(Suppl 3):441–449

    PubMed Central  Article  PubMed  Google Scholar 

  25. N’Go PK, Azzaoui F, Ahami AOT, Soro PR, Najimi M, Chigr F (2013) Developmental effects of Malathion exposure on locomotor activity and anxiety-like behavior in Wistar rat. Health 5(3):603–611

    Article  Google Scholar 

  26. Gandhi DN, Panchal GM, Patel KG (2012) Developmental and neurobehavioral toxicity study of arsenic on rats following gestational exposure. Indian J Exp Biol 50:147–155

    CAS  PubMed  Google Scholar 

  27. Zhao Q, Gadagbui B, Dourson M (2005) Lower birth weight as a critical effect of chlorpyrifos: a comparison of human and animal data. Regul Toxicol Pharmacol 42:55–63

    CAS  Article  PubMed  Google Scholar 

  28. Haave M, Bernhard A, Jellestad FK, Heegaard E, Brattelid T, Lundebye A (2011) Long-term effects of environmentally relevant doses of 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB153) on neurobehavioural development, health and spontaneous behaviour in maternally exposed mice. Behav Brain Funct 7(3):1–14

    Google Scholar 

  29. Murphy HH, Sanusi A, Dilts DR, Djajadisastra M, Hirschhorn N, Yuliantiningsih S (2000) Health effects of pesticide use among Indonesian women farmers. II: reproductive health outcomes. J Agromed 6(4):27–43

    Article  Google Scholar 

  30. Palermo-Neto J, Sakaté M, Flório JC (1997) Developmental and behavioral effects of postnatal amitraz exposure in rats. Braz J Med Biol Res 30:989–997

    CAS  Article  PubMed  Google Scholar 

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Acknowledgments

The authors are very thankful to the Head, Department of Zoology, Punjab Agricultural University Ludhiana, Punjab for providing necessary facilities to carry out the research work.

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.

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Correspondence to Gurinder Kaur Sangha.

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Sharma, D., Sangha, G.K. & Khera, K.S. Effect of Preconceptional Exposure of Triazophos Formulation on Fertility and Reproductive Performance of Female Wistar Rats, Rattus norvegicus . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 85, 987–992 (2015). https://doi.org/10.1007/s40011-014-0460-2

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  • DOI: https://doi.org/10.1007/s40011-014-0460-2

Keywords

  • Preconceptional exposure
  • Sex ratio
  • Developmental milestones
  • Triazophos