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Effect of Dietary Spice Supplementations on Welfare Status of Sea Bass, Dicentrarchus labrax L.

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Abstract

The present study investigated the effects of dietary thyme, rosemary and fenugreek supplementation on welfare status of sea bass, Dicentrarchus labrax L. assessed by blood parameters. Three experimental diets were supplemented with 1 % of the different spices. After 45 days of the feeding trial, the spices did not significantly affect the erythrocyte count, hematocrit ratio, hemoglobin level and superoxide dismutase activity (P > 0.05). However, white blood cell count, neutrophils, monocytes, phagocytic activity, lysozyme activity and myeloperoxidase activity were enhanced in spice treated groups (P < 0.05). Fish fed with the rosemary diet showed a higher respiratory burst activity as opposed to the control group (P < 0.05). Spice-supplemented diets significantly decreased serum glucose, triglyceride, cholesterol, low density lipoprotein, very low density lipoprotein, alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, creatine kinase and lactate dehydrogenase levels (P < 0.05). On the other hand, the total protein, globulin, lipase and high density lipoprotein levels increased (P < 0.05). However, serum bilirubin, phosphorus, magnesium, chloride, calcium and iron were similar to those of the treatment groups (P > 0.05). The results of the present study indicate that dietary supplementation of 1 % thyme, rosemary or fenugreek in commercial diets could improve some hematological, immunological and biochemical status in sea bass hence they can be utilized as immunostimulants and health promoter in fish culture.

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Acknowledgments

The authors would like to thank the Çanakkale Onsekiz Mart University Research Fund for financial assistance (Project Number COMU BAP 2010/79) and Assist. Prof. Dr. Dilek Ülker ÇAKIR from University of Canakkale Onsekiz Mart, Department of Biochemistry, for help in the study. They also thank Assoc. Prof. Dr. Murat KAHVECİ for reviewing the manuscript.

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Authors and Affiliations

  1. Department of Aquaculture, Faculty of Marine Sciences and Technology, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey

    Sevdan Yılmaz & Sebahattin Ergün

  2. Department of Basic Science, Faculty of Marine Sciences and Technology, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Ekrem Şanver Çelik

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  1. Sevdan Yılmaz
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  2. Sebahattin Ergün
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  3. Ekrem Şanver Çelik
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Correspondence to Sevdan Yılmaz.

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Yılmaz, S., Ergün, S. & Çelik, E.Ş. Effect of Dietary Spice Supplementations on Welfare Status of Sea Bass, Dicentrarchus labrax L.. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 229–237 (2016). https://doi.org/10.1007/s40011-014-0444-2

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