Abstract
Allophylus cobbe is a small tree and an ethnomedicinally important plant species belonging to the family Sapindaceae, traditionally used to treat various ailments. Previous work on the species led to show ethanolic extracts to be active against Ranikhet disease virus and have showed effects on central nervous system and hypothermia. However, literature on its polyphenolic content, antioxidant and antimicrobial activity is inadequate. Because of its wide use in traditional medicine, it was decided to evaluate total polyphenols, antioxidant activity and to screen the antimicrobial activity of leaves and stem bark of A. cobbe. Leaves yielded 63.20 mg tannic acid equivalent per gram total phenolics and 17.39 mg quercetin equivalent per gram total flavonoids. A 0.01 % extract was sufficient to produce 32.78 % radical scavenging activity (RSA). 2,2′-Azinobis (3-ethyl benzo thiazoline-6-sulfonic acid) activity using ascorbic acid and Trolox® as standards were higher than ferric reducing antioxidant power activity. Both the extracts were more effective on gram positive organism, than gram negative organisms and were ineffective on fungal strains. The results herein report methanolic leaf and stem bark extracts of A. cobbe to possess good antibacterial and antioxidant activity.
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Acknowledgments
Authors are indebted to Officer-in-Charge, RMRC, ICMR, Belgaum. The present work was supported by Indian Council of Medical Research (ICMR), New Delhi under Grant–SAC 2012/EB/02, through the internal funds of RMRC, Belgaum. All authors are also thankful to Mr. Bhoopal Talawar, lab attendant for his help in collection and processing of plant material.
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An erratum to this article is available at http://dx.doi.org/10.1007/s40011-014-0473-x.
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Ankad, G.M., Upadhya, V., Pai, S.R. et al. Total Polyphenols, Antioxidant and Antimicrobial Activity of Leaves and Stem Bark Extracts of Allophylus cobbe (L.) Raeusch. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 145–149 (2016). https://doi.org/10.1007/s40011-014-0424-6
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DOI: https://doi.org/10.1007/s40011-014-0424-6