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Effect of Ethanolic Extract of Pedalium murex on Growth and Haemato-Immunological Parameters of Labeo rohita

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Abstract

To evaluate the dietary effect of ethanolic extract of Pedalium murex (Bada Gokshru) on growth and haemato-immunological parameters of an Indian major carp, Labeo rohita fingerlings, a 45 days study was conducted. Fishes were fed with diets containing six graded levels (0.0, 0.1, 0.3, 0.7, 1.1 and 1.5 g/100 g diet) of ethanolic extract of P. murex. There was significant variation (p < 0.05) in growth parameters [i.e. net weight gain and specific growth rate (SGR)] and food conversion ratio. The highest weight gain and SGR were recorded as 0.1 g/100 g with P. murex supplemented diet. However, the inclusion of higher levels (1.5 g/100 g diet) of P. murex did not show any significant increase in the growth of fish. Further, the haematological parameters such as packed cell volume, haemoglobin concentration and red blood cells were not significantly different in P. murex supplemented diets as compared to control. A significant (p < 0.05) proliferation of the white blood cells, improvement in respiratory burst activity and lysozyme, lower level of the blood glucose at dietary level of 0.1 g/100 g were observed. The results of present study explain the role of ethanolic extract of P. murex in growth and immune defence mechanism of fish.

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References

  1. Mukherjee PK (2002) Quality control of herbal drugs: an approach to evaluation of botanicals. Business Horizons Pharmaceutical, New Delhi, p 800

    Google Scholar 

  2. Shukla VN, Khanuja SPS (2004) Pharmacology and botanical studies on Pedalium murex. J Med Aromat Plant Sci 26:64–69

    CAS  Google Scholar 

  3. Shelke TT, Kothai R, Adkar PP, Bhaskar VH, Juvale KC, Kamble BB (2009) Nephroprotective activity of ethanolic extract of dried fruits of Pedalium murex linn. J cell tissue Res 9:1687–1690

    Google Scholar 

  4. Vibhuti D, Tripathi AK (2009) Herbaceous remedial plants of district Haridwar, Uttarakhand (India). Adv Plant Sci 22:589–594

    Google Scholar 

  5. Upadhayay B, Singh KP, Kumar A (2011) Ethno-veterinary uses and informants consensus factor of medicinal plants of Sariska region, Rajasthan, India. J Ethnopharmacol 133:14–25

    Article  Google Scholar 

  6. Balasubramanian MN, Muralidharan P, Balamurugan G (2008) Antihyperlipidemic activity of Pedalium murex (Linn.) fruits on high fat diet fed rats. Int J Pharmacol 4:310–313

    Article  Google Scholar 

  7. Soosairaj S, Natarajan D, Nagamurugan N, Radha B (2008) Aspergillosidal effect of Pongamia glabra and Pedalium murex- a comparative study. J Phytol Res 21:221–224

    Google Scholar 

  8. Balamurugan G, Murlidharan P, Polapala S (2010) Aphrodisiac activity and curative effect of Pedalium murex (L.) against ethanol induced infertility in male rats. Turkish J Biol 34:153–163

    Google Scholar 

  9. Srinivas P, Venkateshwarlu L, Kumar ACH (2011) Antioxidant activity of Pedalium murex fruits in carbon tetrachloride- induced hepatopathy in rats. Int J Pharm Biosci 2:622–628

    Google Scholar 

  10. Anon (2004) The ayurvedic pharmacopoeia of India, p. 232

  11. Puri HS (2003) Rasayana-ayurvedic herbs for longevity and Rejuvination. Taylor and Francies, London, p 263

    Book  Google Scholar 

  12. Venkatarathina KT, Muthusamy VA, Ramanathan S (2005) Evaluation of the petroleum ether extracts of Pedalium murex against Japanese encephalitis vector culex Tritaenlorhynchus. Antiseptic 102:335–336

    Google Scholar 

  13. Muralidharan P, Balamurugan G (2008) Analgesic and anti-inflammatory activities of aqueous extract of Pedalium murex Linn. Biomedicine 28:84–87

    Google Scholar 

  14. Subramanian SS, Nair AGR (1972) Flavonoids of the leaves of Pedalium murex Linn. Phytochemistry 11:464–465

    Article  CAS  Google Scholar 

  15. Prasad TNV, Sastry KV (1998) A note on the chemical examination of Pedalium murex Linn. leaves. Indian Drugs 25:84–85

    Google Scholar 

  16. Shukla YN, Thakur RS (1983) Heptatriacontan-4-one, Tetratriacontanyl octacosanoate and other constituents from Pedalium murex Linn. Phytochemistry 22:973–974

    Article  CAS  Google Scholar 

  17. Harvey SK (1967) A brief comparative pharmacognostic study of certain indigenous drugs. Nat Med J 9:519

    Google Scholar 

  18. Mangle MS, Jolley CI (1998) HPTLC studies on Tribulus terrestris (Chhota gokhru) and Pedalium murex (Bada gokhru). Indian Drugs 35:189–194

    CAS  Google Scholar 

  19. Bhakuni RS, Shukla YN, Thakur RS (1992) Flavonoids and other constituents from Pedalium murex Linn. Phytochemistry 31:2917–2918

    Article  CAS  Google Scholar 

  20. Rastogi JN, Sharma OD, Loiwal SD (1982) Amino acids in certain medicinal plants. Bull Pure Appl Sci 1:11–12

    CAS  Google Scholar 

  21. Kavitha K, Sharma LL (1996) Use of herb (Asparagus recemous Wild) supplemented diet for promoting growth in the fry of Labeo rohita. The fourth Indian Fisheries Forum, Kochi, p 136

    Google Scholar 

  22. Rajendra KR (1990) Prevention and control of fish diseases by herbal medicine. Fish Health Sect News 3:3–4

    Google Scholar 

  23. Dierekbusarakom S (2004) Application of medicinal herbs to aquaculture in Asia. Walailak J Sci Technol 1(1):7–14

    Google Scholar 

  24. Harborne JB (1973) Phytochemical methods. In: A guide to modern techniques of analysis. Chapman and Hall, London

  25. Jobling M (1983) A short critique of methodology used in fish growth and nutrition studies. J Fish Biol 23:685–703

    Article  Google Scholar 

  26. AOAC (1995) Official methods of analysis of AOAC international vol. 1 agriculture chemicals; contaminants, drugs, 16th edn. AOAC International, Arlington

    Google Scholar 

  27. Nelson-Somogyi, 1944, 1945, cited by Oser Bl (1965) ln: Hawk’s physiological Chemistry, 14th edn. McGraw Hill, New York

  28. Secombes CJ (1990) Isolation of salmonid macrophage and analysis of their killing activity. ln: Stolen JSTC, Fletcher DP, Anderson BS, WB Van, M Winkel (Eds) Techniques in fish immunology. SOS Publication, New Jersey

  29. Stasiack AS, Bauman CP (1996) Neutrophil activity as a potent indicator for concomitant analysis. Fish Shellfish Immunol 37:539

    Google Scholar 

  30. Kaleeswaran B, Ilavenil S, Ravikumar S (2011) Growth response, Feed conversion ratio and Antiprotease activity of Cynodon dactylon (L.) Mixed diet in Catla catla (Ham.). J Animal Veterinary Adv 10(4):517–518

    Google Scholar 

  31. Prasad G, Mukhthiraj S (2011) Effect of methanolic extract of Andrographis paniculata (Nees) on growth and haematology of Oreochromis mossambicus (Peters). World J Fish Marine Sci 3(6):473–479

    Google Scholar 

  32. Sanchez JAO, Flores AC, Hernandez JRO (2009) The effect of herbal growth promoter feed additive on Shrimp performance. Res J Biol Sci 4(9):1022–1024

    Google Scholar 

  33. Kour D, Sharma LL, Sharma BK (2004) Use of Bala (Sida cordifolia Linn.) as growth promoter in the supplementary feed of Cirrhinus mrigala (Ham.). Indian J Fish 51(4):501–504

    Google Scholar 

  34. Siddhuraju P, Becker K (2001) Preliminary nutritional evaluation of Mucuna seed meal (Mucuna pruriens var.utilis) in common carp (Cyprinus carpio L.): an assessment by growth performance and feed utilization. Aquaculture 196:105–123

    Article  Google Scholar 

  35. Siddhuraju P, Becker K (2003) Comparative nutritional evaluation of differentially processed mucuna seeds (Mucuna pruriens (L.) DC var. utilis (Wallex Wight) Baker ex Burck) on growth performance, feed utilization and body composition in Nile tilapia (Oreochromis niloticus L.). Aquac Res 34:487–500

    Article  CAS  Google Scholar 

  36. Adeyemo OK (2005) Haematological and histopathological effects of cassava mill effluent in Clarias gariepinus. Afr J Biomed Res 8(3):179–183

    Google Scholar 

  37. Omoniyi I, Agbon AO, Sodunke SA (2002) Effect of lethal and sublethal concentrations of tobacco (Nicotiana tobaccum) leaf dust extract on weight and hematological changes in Clarias gariepinus (Burch.). J Appl Sci Environ Manage 6(2):37–41

    Google Scholar 

  38. Gabriel UU, Obomanu FG, Edori OS (2009) Haematology, Plasma enzymes and organ indices of Clarias gariepinus after intramuscular injection with aqueous leaves extracts of Lepidagathis alopecuroides. Afr J Biochem Res 3(9):312–316

    CAS  Google Scholar 

  39. Sahu S, Das BK, Pradhan J, Mohapatra BC, Mishra BK, Sarangi NN (2007) Effect of Mangifera indica kernel as feed additive on immunity and resistance to Aeromonas hydrophila in Labeo rohita fingerlings. Fish Shellfish Immunol 23:109–118

    Article  PubMed  CAS  Google Scholar 

  40. Chukwudi NH, Siemon O, Chinyere AJ (2011) Analysis of some biochemical and Haematological parameters for Mucuna pruriens (DC) seed powder in male rats. Pakistan J Pharm Sci 24(4):523–526

    Google Scholar 

  41. Rajaram N, Janardhanan K (1991) The biochemical composition and nutritional potential of the tribal pulse, Mucuna gigantean (Wildd) DC. Plant Foods Hum Nutr 44:45–51

    Article  Google Scholar 

  42. Coleseh (1986) Veterinary clinical pathology. Saunders, Philadelphia, p 615

    Google Scholar 

  43. Suresh RN, Amolkumar H (2009) Evaluation of Immunomodulatory activity of an extract of Andrographolides from Andrographis paniculata. Thiemee J planta Med 75(8):785–791

    Article  Google Scholar 

  44. Secombes CJ, Fletcher TC (1992) The role of phagocytes in the protective mechanisms of fish. Ann Rev Fish Dis 2:53–71

    Article  Google Scholar 

  45. Sharp GJE, Secombes CJ (1993) The role of reactive oxygen species in the killing of the bacterial fish pathogen Aeromonas salmonicida by rainbow trout macrophages. Fish Shellfish Immunol 3:119–129

    Article  Google Scholar 

  46. Dugenci SK, Arda N, Candan A (2003) Some medicinal plants as immunostimulant for fish. J Ethnopharmacol 88:99–106

    Article  PubMed  Google Scholar 

  47. Chen X, Wu Z, Yin J, Li L (2003) Effects of four species of herbs on immune function of Carassius auratus gibelio. Journal of Fish Sciences of China 10:36–40 (China)

    Google Scholar 

  48. Neumann NF, Stafford JL, Barreda D, Ainsworth AJ, Belosevic M (2001) Antimicrobial mechanisms of fish phagocytes and their role in host defence. Dev Comp Immunol 25:807–825

    Article  PubMed  CAS  Google Scholar 

  49. Seeley KR, Gillespie PD, Weeks BA (1990) A simple technique for the rapid spectrophotometric determination of phagocytosis by fish macrophages. Marine Environ Res 30:123–128

    Article  Google Scholar 

  50. Ainsworth AJ (1994) A beta-glucan inhibitable zymosan receptor on channel catfish eutrophils. Vet Immunol Immunopathol 41:141–152

    Article  PubMed  CAS  Google Scholar 

  51. Siwicki AK, Anderson DP, Rumsey GL (1994) Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vet Immunol Immunopathol 41:125–139

    Article  PubMed  CAS  Google Scholar 

  52. Jeney G, Galeotti M, Volpatti D, Jeney Z, Anderson DP (1997) Prevention of stress in rainbow trout (Oncorhynchus mykiss) fed diets containing different doses of glucan. Aquaculture 154:1–15

    Article  CAS  Google Scholar 

  53. Metwally MAA (2009) Effects of garlic (Allium sativum) on some antioxidant activities in tilapia nilotica (Oreochromis niloticus). World J Fish Marine Sci 1(1):56–64

    CAS  Google Scholar 

  54. Kumar GR, Reddy KP (1999) Reduced nociceptive responses in mice with alloxan induced hyperglycemia after garlic (Allium sativum Linn.) treatment. Indian J Exp Biol 37:662–666

    PubMed  CAS  Google Scholar 

  55. Thomson M, Ali M (2003) Garlic (Allium sativum): a review of its potential use as an anticancer agent. Curr Cancer Drug Targ 3:67–81

    Article  CAS  Google Scholar 

  56. Sheela CG, Augusti KT (1992) Antidiabetic effects of S-allyl cysteine sulphoxide isolated from garlic, Allium sativum Linn. Indian J Exp Biol 30:523

    PubMed  CAS  Google Scholar 

  57. Alexander JB, Ingram GA (1992) Non-cellular non-specific defence mechanisms of fish. Ann Rev Fish Dis 2:249–279

    Article  Google Scholar 

  58. Jian J, Wu Z (2003) Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson). Aquaculture 218:1–9

    Article  Google Scholar 

  59. Jian J, Wu Z (2004) Influences of traditional Chinese medicine on nonspecific immunity of Jian carp (Cyprinus carpio var. Jian). Fish Shellfish Immunol 16:185–191

    Article  PubMed  Google Scholar 

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Acknowledgement

The authors would like to acknowledge the Director, Central Institute of Fisheries Education (CIFE), Mumbai, ICAR, New Delhi, and Vice Chancellor, Maharana Pratap University of Agriculture and Technology Udaipur, Rajasthan, India for providing facilities for the research work.

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Authors and Affiliations

  1. Aquaculture Division, Central Institute of Fisheries Education, Panch Marg Off Yari Road, Versova Andheri(W), Mumbai, 400061, India

    Manohar Lal Ojha, Narinder Kumar Chadha, Chandra Prakash Gupta & Paramita Banerjee Savant

  2. Aquaculture Research Unit, Directorate of Research, Maharana Pratap University of Agriculture and Technology, Udaipur, 313001, Rajasthan, India

    Ved Prakash Saini

  3. Central Agriculture Research Institute, P.O. Box No. 181, Portblair, Andaman and Nicobar Island, India

    Sibnarayan Damroy

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  1. Manohar Lal Ojha
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Correspondence to Manohar Lal Ojha.

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Ojha, M.L., Chadha, N.K., Saini, V.P. et al. Effect of Ethanolic Extract of Pedalium murex on Growth and Haemato-Immunological Parameters of Labeo rohita . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 997–1003 (2014). https://doi.org/10.1007/s40011-013-0282-7

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