Variations in Physiological Response, Lipid Peroxidation, Antioxidant Enzyme Activities, Proline and Isoflavones Content in Soybean Varieties Subjected to Drought Stress
- 543 Downloads
Drought stress is one of the crucial factors affecting the global plant growth and crop productivity. In the present study, Indian soybean varieties (cv. JS 335 and cv. KHSb-2) were examined for the changes in their antioxidant enzyme activity, total isoflavone content and also the influence on its growth under drought stress. Several physiological responses, malondialdehyde, proline contents and antioxidant activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and glutathione reductase were estimated under mild drought stress and recovery stage respectively. Mild water deficit stress for 5 days during late vegetative stage increased total superoxide dismutase activity in KHSb-2 than in JS 335 variety. Water deficit stress triggered the activities of catalase, peroxidase, ascorbate peroxidase and glutathione reductase enzymes in KHSb-2 than in JS 335. High accumulation of proline and lower level of lipid peroxidation were also observed in KHSb-2 than in JS 335. The root and shoot fresh and dry weight along with leaf relative water content of cultivar KHSb-2 exhibited lesser reduction than in JS 335. The partial recovery of antioxidant enzyme activities, elevated relative water content and growth parameters in both varieties were compared to stress plants. Moderate enhancement in total isoflavone content was observed in KHSb-2 (3.44 % increase) and JS 335 (2.84 % increase) in seeds subjected to mild stress. From nutraceutical point of view, the results investigated would be vital for development of soybean cultivar with drought tolerance and moderate enhancement of isoflavone content under drought stress.
KeywordsDrought Soybean Superoxide dismutase Peroxidase Catalase Isoflavone
The author M K Akitha Devi is thankful to CSIR, New Delhi for the fellowship award.
- 6.Masoumi I, Masoumi M, Darvish F, Daneshian JJ, Nourmohammadi G, Habibi D (2010) Change in several antioxidant enzymes activity and seed yield by water deficit stress in soybean (Glycine max L.) varieties. Not Bot Horti Agrobo 38:86–94Google Scholar
- 11.Madani A, Nehal M, Haque SS, Khan A (2010) Perspective of oxidative stress in a biological system and prevention by naturally occurring antioxidant. Proc Natl Acad Sci India Sect B Biol Sci 80:287–295Google Scholar
- 25.Slavick B (1979) Methods of studying plant water relations. Springer-Verlag, New YorkGoogle Scholar
- 29.Luck H (1974) Catalases. In: Bergmeyer HU (ed) Methods in enzymatic analysis, vol 2. Academic Press, New YorkGoogle Scholar
- 31.Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
- 35.Anjum SA, Xie XY, Wang LC, Saleem MF, Man C, Lei W (2011) Morphological, physiological and biochemical responses of plants to drought stress. Afr J Agric Res 6:2026–2032Google Scholar
- 36.Lobato AKS, Neto MAM, Meirelles ACS, Silva LI, Marochio CA, Monteiro ER, Neto HZ, Maleia MP, Moiana LD, Bronzato AR, Cruz FJR (2009) Relationship between leaf relative water content and total soluble proteins in soybean exposed to short water deficit. Res J Biol Sci 4:1061–1067Google Scholar
- 39.Lobato AKD, Neto CFD, Filho BGD, da Costa RCL, Cruz FJR, Neves HKB, Lopes MJD (2008) Physiological and biochemical behavior in soybean (Glycine max cv. Sambaiba) plants under water deficit. Aust J Crop Sci 2:25–32Google Scholar
- 42.Upadhyay RK, Sharma GD, Panda SK (2011) Responses of antioxidant metabolism and defense mechanism of aquatic macrophyte, Pistia stratiotes L. to zinc treatment under copper stress. Proc Natl Acad Sci India Sect B Biol Sci 81:422–427Google Scholar