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Identification of RAPD Markers Associated with Morphological, Biochemical and Ionomic Characteristics in Indian Tomato Genotypes

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Random Amplified Polymorphic DNA (RAPD) technique is one of the simplest marker techniques used in the differentiation of organisms. In the present study, 28 Indian tomato genotypes along with morphological, biochemical and ionomic characteristics were subjected to RAPD analysis. Ten random primers were selected from 100 operon primers which produced 88 reproducible bands; 89.8 % (79) of bands were polymorphic and 10.2 % (9) were monomorphic. The total number of markers identified by SMA in different parameters were plant height-06, fruit number-07, fruit cluster-03, fruit weight-03, pH-03, titratable acidity-03, TSS-03, total sugars-07, reducing sugars-06, non-reducing sugars-02, and lycopene-05. In case of macroelements, the total number of markers identified in SMA was P-03, K-05, Ca-06, Mg-03; for microelements, Mn-03, Fe-01, Co-06, Ni-06, Cu-04, Bo-06, Na-04, Mo-07, Cd-02 and Li-06. The total markers associated by SMRA showed the following. Plant height-06, fruit number-11, fruit cluster-06, fruit weight-17, pH-03, titratable acidity-04, TSS-04, total sugars-19, reducing sugars-20, non-reducing sugars-14, lycopene-19, P-13, K-18, Ca-03, Mg-03, Iron-13, Co-04, Ni-13, Cu-16, Zn-26, Bo-22, Na-21, Mo-20, Cd-18 and Li-12. The study suggested that the RAPD markers identified could be used in breeding elite tomato genotypes with good characteristics.

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Acknowledgments

The authors gratefully acknowledge Dr. David Salt for ionomic estimation of 33 Indian genotypes of Tomato at Purdue University, West Lafayette, USA. They also wish to thank Dr. Avtar K Handa and Dr. K. G. Raghothama and USDA for all their valuable inputs and funding of USAID Project. Special thanks are due to Ms. Shuba Karanth and Mr. Santosh Rathod for helping in conducting statistical analysis. The first author thanks Kuvempu University for giving the opportunity to pursue the doctoral study.

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Authors and Affiliations

  1. Department of Applied Botany, Kuvempu University, Shankaraghatta, Shimoga, 577451, Karnataka, India

    Somayaji Prarthana & M. B. Shivanna

  2. Department of Biotechnology, University of Agricultural Sciences, GKVK, Bangalore, 560065, Karnataka, India

    D. Theertha Prasad

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  1. Somayaji Prarthana
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  2. D. Theertha Prasad
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  3. M. B. Shivanna
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Correspondence to M. B. Shivanna.

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Prarthana, S., Prasad, D.T. & Shivanna, M.B. Identification of RAPD Markers Associated with Morphological, Biochemical and Ionomic Characteristics in Indian Tomato Genotypes. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 55–64 (2014). https://doi.org/10.1007/s40011-013-0202-x

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  • DOI: https://doi.org/10.1007/s40011-013-0202-x

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