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Bacteria-induced systemic resistance and growth promotion in Glycine max L. Merrill upon challenge inoculation with Fusarium oxysporum

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

In the present study, two bacterial strains along with Pseudomonas sp. strain VS1 were employed and screened on the basis of plant growth-promotion characteristics. Strains showed in vitro antibiosis against the test fungal pathogen Fusarium oxysporum together with plant growth-promotion properties that included indole-3-acetic acid, 1-aminocyclopropane-1-carboxylic acid and inorganic phosphorus solubilization production. The activities of stress responsive enzymes that included lipoxygenase, phenylalanine ammonia-lyase, catalase, peroxidase and superoxide dismutase were reported in bacteria and control-treated soybean plants. Bacteria treated seeds showed higher enzymatic activities in leaves in comparison with fungal spore treated seeds and were considered as positive control. Treatment with bacteria resulted in remarkable increase in the chlorophyll content in leaves as compared to water (negative control) and fungal spore treated seeds (P < 0.05). Soybean seeds treated with bacteria were subjected to fungal spore stress at 0 day after seeding, strain SJ5 significantly enhanced emergence at 28 days after seeding than other treated seeds and positive control. The fresh weight of both shoot and root of plant was significantly increased when soybean seeds were treated with bacteria in the presence of fungal spores as compared to positive control. The dry weights of both roots and shoots were significantly increased by the bacterial treatments as compared to positive control (P < 0.05). Strain treated seeds significantly enhanced root and shoot length together with a number of lateral roots when spore suspension was applied at 0 day after seeding. Moreover, soybean plants treated with strain SJ5 exhibited significantly greater root and shoot weights as compared to the other treated plants when fungal spore suspension was applied at 0 day after seeding.

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References

  1. Sun HY, Deng SP, Raun WR (2004) Bacterial community structure and diversity in a century-old manure-treated agro-ecosystem. Appl Environ Microbiol 70:5868–5874

    Article  PubMed  CAS  Google Scholar 

  2. Choudhary DK, Sharma KP, Gaur RK (2011) Biotechnological perspectives of microbes in agro-ecosystems. Biotechnol Lett 33:1905–1910

    Article  PubMed  CAS  Google Scholar 

  3. Choudhary DK (2012) Microbial rescue to plant under habitat-imposed abiotic and biotic stresses. Appl Microbiol Biotechnol 96:1137–1155

    Article  PubMed  CAS  Google Scholar 

  4. Choudhary DK, Prakash A, Johri BN (2007) Induced systemic resistance (ISR) in plants: mechanism of action. Indian J Microbiol 47:289–297

    Article  PubMed  CAS  Google Scholar 

  5. Choudhary DK (2011) Plant growth-promotion (PGP) activities and molecular characterization of rhizobacterial strains isolated from soybean (Glycine max L. Merril) plants against charcoal rot pathogen, Macrophomina phaseolina. Biotechnol Lett 33:2287–2295

    Article  PubMed  CAS  Google Scholar 

  6. Choudhary DK, Johri BN (2009) Interactions of Bacillus spp. and plants—With special reference to induced systemic resistance (ISR). Microbiol Res 164:493–513

    Article  PubMed  CAS  Google Scholar 

  7. Hass D, Keel C, Reimann C (2002) Signal transduction in plant beneficial rhizobacteria with biocontrol properties. Antonie van Leeuwenhoek 81:385–395

    Article  Google Scholar 

  8. van Loon LC, Bakker PAHM, Pieterse CMJ (1998) Systemic resistance induced by rhizosphere bacteria. Annu Rev Phytopathol 36:453–483

    Article  PubMed  Google Scholar 

  9. Yan Z, Reddy MS, Ryu CM, Mc Inroy JA, Wilson M, Kloepper JW (2002) Induced systemic protection against tomato late blight elicited by plant growth-promoting rhizobacteria. Phytopathology 92:1329–1333

    Article  PubMed  CAS  Google Scholar 

  10. Chauhan SK (1963) Influence of different soil temperatures on the incidence of fusarium wilt of gram (Cicer arietinum L.). Proc Indian Acad Sci B 33:552–554

    Google Scholar 

  11. Sharma A, Johri BN, Sharma AK, Glick BR (2003) Plant growth-promoting bacterium Pseudomonas sp. strain GRP3 influences iron acquisition in Mung bean (Vigna radiata L. Wilzeck). Soil Biol Biochem 35:887–894

    Article  CAS  Google Scholar 

  12. Hiscox JD, Israelstam GF (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334

    Article  CAS  Google Scholar 

  13. Lowry OH, Rosebrovgh NJ, Farr AC, Randall RJ (1951) Protein measurements with Folin phenol reagent. Biol Chem 193:265–275

    CAS  Google Scholar 

  14. Axelrod B, Cheesbrough TM, Laakso S (1981) Lipoxygenase from soybean. Methods Enzymol 71:441–451

    Article  CAS  Google Scholar 

  15. Pascholati SF, Nicholson RL, Butler DL (1986) Phenylalanine ammnia-lyase activity and anthocyanin accumulation in wounded maize mesocotyls. Phytopathology 115:165–172

    Article  CAS  Google Scholar 

  16. Allain CC, Poon LC, Chan CSG, Richmond W, Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20:470–475

    PubMed  CAS  Google Scholar 

  17. Patterson BD, Payne LA, Chen Y, Graham D (1984) An inhibitor of catalase induced by cold chilling-sensitive plants. Plant Physiol 76:1014–1018

    Article  PubMed  CAS  Google Scholar 

  18. Beyer WF Jr, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566

    Article  PubMed  CAS  Google Scholar 

  19. Kloepper JW (1991) Plant growth-promoting rhizobacteria as biological control agents in soilborne diseases. In: Bay-Paterson J (ed) The biological control of plant diseases, Taipei, pp 142–152

  20. Broetto F, Marchese JA, Leonardo M, Regina M (2005) Fungal elicitor-mediated changes in polyamine content, phenylalanine ammonia-lyase and peroxidase activities in bean cell culture. Gen Appl Plant Physiol 31:235–246

    Google Scholar 

  21. Croft KPC, Voisey CR, Slusarenkl AJ (1990) Mechanism of hypersensitive cell collapse: correlation of increase lipoxygenase activity with membrane damage in leaves of Phaseolus vulgaris cv. Red Maxican inoculated with avirulent race/cells of Pseudomonas syringae pv. Phaseolicola. Physiol Mol Plant Pathol 36:49–62

    Article  CAS  Google Scholar 

  22. Gutierrez-Manero FJ, Ramos-Solano B, Probanza A, Mehouachi J (2001) The plant growth-promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberlins. Physiol Plantarum 111:206–211

    Article  Google Scholar 

  23. Ray H, Douches DS, Hammerschmidt R (1998) Transformation of potato with cucumber peroxidase: expression and disease response. Physiol Mol Plant Pathol 53:93–103

    Article  CAS  Google Scholar 

  24. Hammerschmidt R (1999) Induced disease resistance: how do induced plants stop pathogens? Physiol Mol Plant Pathol 55:77–84

    Article  CAS  Google Scholar 

  25. Podile AR, Lakshmi VDV (1998) Seed bacterization with Bacillus subtilis increases phenylalanine ammonia-lyase and reduces the incidence of fusarium wilt of pigeonpea. Phytopathol 146:255–259

    Article  CAS  Google Scholar 

  26. Liang X, Dron M, Schmid J, Dixon R, Lamb C (1989) Developmental and environmental regulation of a phenylalanine ammonia-lyase-β-glucuronidase gene fusion in transgenic tobacco plants. Proc Natl Acad Sci 86:9284–9288

    Article  PubMed  CAS  Google Scholar 

  27. Benhamou N, Kloepper JW, Tuzun S (1998) Induction of resistance against Fusarium wilt of tomato by combination of chitosan with an endophytic bacterial strain: ultrastructure and cytochemistry of the host response. Planta 204:153–168

    Article  CAS  Google Scholar 

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Acknowledgments

Authors gratefully acknowledge DBT and DST-SERB Grant no. BT/PR1231/AGR/21/340/2011 and SR/FT/LS-129/2012 respectively to DKC for financial support.

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Correspondence to Devendra Kumar Choudhary.

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Jain, S., Vaishnav, A., Kasotia, A. et al. Bacteria-induced systemic resistance and growth promotion in Glycine max L. Merrill upon challenge inoculation with Fusarium oxysporum . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 83, 561–567 (2013). https://doi.org/10.1007/s40011-013-0172-z

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