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Distribution of Gastrin Immune Reactive Cells in the Alimentary Canal of a Stomachless Hill Stream Loach, Lepidocephalus guntea (Hamilton): An Immuno-Histochemical study

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Abstract

The presence of gastrin producing enteroendocrine cells characteristic of so called stomach were also present in stomachless loach Lepidocephalus guntea (Hamilton). The immunohistochemical response reveals their presence in the anterior and middle regions of the intestine. Both open and closed type of cells have been recorded but the former type predominates. Some of the open type of gastrin immune reactive cells, amidst mucosal epithelium have been observed to release their content into the lumen of the intestine, suggesting hormone–parahormone–lumone role of gastrin. The closed type of gastrin immunoreactive cells are mostly located in the sub-mucosal region with characteristic cytoplasmic extensions projecting towards both lumen as well as basal zone of the villi. Keeping their morphological appearance in consideration, the present authors propose that both the types of cells are interconvertible as per their requirement as hormone or parahormone or lumone.

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References

  1. Fange R, Grove D (1979) Digestion. In: Hoar WS, Randall DJ, Brett JR (eds) Fish Physiology, vol 8. Academic Press, New York, pp 162–260

    Google Scholar 

  2. Moyes CD, Schulte PM (2007) Digestion. In: Principles of Animal Physiology. Pearson Education, New Delhi, pp 536–579

  3. Smith DM, Grasty RC, Theodosion NA, Tabin CJ, Nascone-Yoder NM (2000) Evolutionary relationship between the amphibian, avian and mammalian stomachs. Evolu and Develop 2(6):348–359

    Article  CAS  Google Scholar 

  4. Secor S (2005) Evolutionary and cellular mechanisms regulating intestinal performance of amphibians and reptiles. Integr Comp Biol 45:282–294

    Article  PubMed  Google Scholar 

  5. Walker WF, Liem KF (1994) Functional Anatomy of the Vertebrates: An Evolutionary Prospective. Saunders College Publishing, New York

    Google Scholar 

  6. Takei Y, Loretz CA (2011) The gastrointestinal tract as an endocrine/neuroendocrine/paracrine organ: organization, chemical messengers and physiological targets. In: “Multi-functional Gut of fish (Fish Physiology, Vol. 30). Elsvier, USA. pp 260–317

  7. Feyrter F (1938) Ober diffuse endoknne epitheliale Organe. Barth, Leipzig

    Google Scholar 

  8. Pearse AGE, Takor T (1979) Embryology of the diffuse neuroendocrine system and its relationship to the common peptides. Fed Proc Fed A Soc Exp Biol 38:2288–2294

    CAS  Google Scholar 

  9. Edkins JS (1905) On the chemical mechanism of gastric secretion. Proc Roy Soc Lond (B) 76:376

    Article  Google Scholar 

  10. Edkins JS (1906) The chemical mechanism of gastric secretion. J Physiol 34:133–144

    PubMed  Google Scholar 

  11. Baylsis WM, Starling EH (1902) The mechanism of pancreatic secretion. J Physiol (Lond) 28:325–353

    Google Scholar 

  12. Ivy AC, Oldberg E (1928) A hormone mechanism for gallbladder contraction and evacuation. Am J Physiol 86:599–613

    CAS  Google Scholar 

  13. Inui A, Asakawa A, Bowers CY et al (2004) Ghrelin, appetite, and gastric motility: the emerging role of the stomach as an endocrine organ. FASEB J 18(3):439–456

    Article  PubMed  CAS  Google Scholar 

  14. Roux CW, Ghatei MA, Gibbs JSR, Bloom SR (2005) The putative satiety hormone PYY is raised in cardiac cachexia associated with primary pulmonary hypertension. Heart 91:241–242

    Article  PubMed  Google Scholar 

  15. Hassouna R, Zizzari P, Tolle V (2010) The ghrelin/obestatin balance in the physiological and pathological control of growth hormone secretion, body composition and food intake. J Neuroendocrinol 22(7):793–804

    PubMed  CAS  Google Scholar 

  16. Wynne K, Field BC, Bloom SR (2010) The mechanism of action for oxyntomodulin in the regulation of obesity. Curr Opin Investig Drugs 11(10):1151–1157

    PubMed  CAS  Google Scholar 

  17. Ahlman H, Nilsson O (2001) The gut as the largest endocrine organ of the body. Ann Oncol 12(S2):S63–S68

    Article  PubMed  Google Scholar 

  18. Johnson LR (1977) Gastrointestinal hormones and their functions. Ann Rev Physiol 39:135–158

    Article  CAS  Google Scholar 

  19. Dawson IMP (1978) Visualization of the diffused endocrine system. J Clin Path 33(8):7–11

    Article  Google Scholar 

  20. Holmgren S, Olsson C (2009) The neuronal and endocrine regulation of gut function. In: Bernier NJ, Van Der Kraak G, Farell AP, Brauner CJ (eds) Fish Neuroendocrinology. Academic Press, Amsterdam, pp 467–512

    Chapter  Google Scholar 

  21. Jordan P Jr, Yip BS (1972) The presence of gastrin in fasting and stimulated gastric juice of man. Surgery 72:352–356

    PubMed  Google Scholar 

  22. Uvnas-Wallensten K (1977) Occurrence of gastrin in gastric juice, in antral secretion and in antralperfusates of cats. Gastroenterology 73:487–491

    PubMed  CAS  Google Scholar 

  23. Kapoor BG, Smit H, Verighina IA (1975) The alimentary canal and digestion in teleosts. Adv Mar Biol 13:109–239

    Article  CAS  Google Scholar 

  24. Kobegenova SS (1988) On a possible cause of reduction of the stomach in some teleosts. Vopr Ikhtiol 6:983–992

    Google Scholar 

  25. Dockray GJ, Varro A, Dimaline R, Wang T (2001) The gastrins: their production and biological activities. Annu Rev Physiol 63:119–139

    Article  PubMed  CAS  Google Scholar 

  26. Danzer M, Jocic M, Samberger C, Painsipp E, Bock E, Pabst MA, Crailsheim K, Schicho R, Lippe IT, Holzer P (2004) Stomach brain communication by vagal afferents in response to luminal acid back diffusion, gastrin, and gastric acid secretion. Am J Physiol 286:G403–G411

    CAS  Google Scholar 

  27. Guilloteau P, Le Meuth-Metzinger V, Morisset J, Zabielski R (2006) Gastrin, cholecystokinin and gastrointestinal tract functions in mammals. Nutr Res Rev 19:254–283

    Article  PubMed  CAS  Google Scholar 

  28. Kaise H, Vine N (1998) The effect of 2-phenoxyethanol and transport packing density on post transport survival rate and metabolic activity in the gold fish Carassius auratus. Aquarium sci conserv 2:1–7

    Article  Google Scholar 

  29. Brown D, Lydon J, McLaughlin M, Stuart-Tilley A, Tyszkowski R, Alper S (1996) Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS). Histochem Cell Biol 105(4):261–267

    Article  PubMed  CAS  Google Scholar 

  30. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580

    Article  PubMed  CAS  Google Scholar 

  31. Ishizuka J, Martinez J, Townsend CM Jr, Thompson JC (1992) The effect of gastrin on growth of human stomach cancer cells. Ann Surg 215(5):528–534

    Article  PubMed  CAS  Google Scholar 

  32. Jain RN, Samuelson LC (2006) Differentiation of the Gastric Mucosa II. Role of gastrin in gastric epithelial cell proliferation and maturation. Am J Physiol Gastrointest Liver Physiol 291(5):G762–G765

    Article  PubMed  CAS  Google Scholar 

  33. Abad M, Binkhorst F, Elbal M, Rombout JH (1987) A comparative immunocytochemical study of the gastroentero-pancreatic (GEP) endocrine system in a stomachless and a stomach–containing teleost. Gen Comp Endocrinol 66(1):123–136

    Article  PubMed  CAS  Google Scholar 

  34. Fang Z, Yamada J, Pan Q (1988) Immunohistochemical localization of peptide hormones in endocrine cells of the gut of a stomachless teleost fish, Grass Carp. J Huazhong Agric Univ 7:169–175

    Google Scholar 

  35. Patricia B, Holmgren S (1989) A comparative study of neuropeptides in the intestine of two stomachless teleosts (Poecilia reticulata, Leuciscus idus melanotus) under conditions of feeding and starvation. Cell Tissue Res 255(1):245–254

    Google Scholar 

  36. Pan QS, Fang ZP, Zhao YX (2000) Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes. World J Gastroenterol 6(1):96–101

    PubMed  Google Scholar 

  37. Kuru N, Cinar K, Senol N, Demirbag E, Diler D (2010) Endocrine cells in the gastrointestinal tract of Garra rufa. Kafkas Univ Vet Fak Derg 16(B):235–241

    Google Scholar 

  38. Wilson JM, Castro LFC (2011) Morphological diversity of the gastrointestinal tract in fishes. In: “Multifunctional Gut of fish” (Fish Physiology), Vol. 30. Elsevier, New York, pp. 1–55

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Acknowledgments

One of the authors Ritesh Kumar Shukla is thankful to UGC Eastern Regional Office, Kolkata for funding the project as minor research project (F. PSJ-011/10-11). We are also grateful to the authorities of St. Xavier’s College, Ranchi for the research facilities.

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  1. St. Xavier’s College, Ranchi, Ranchi, Jharkhand, India

    N. Venkat Appa Rao & Ritesh Kumar Shukla

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  1. N. Venkat Appa Rao
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  2. Ritesh Kumar Shukla
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Correspondence to N. Venkat Appa Rao.

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Venkat Appa Rao, N., Shukla, R.K. Distribution of Gastrin Immune Reactive Cells in the Alimentary Canal of a Stomachless Hill Stream Loach, Lepidocephalus guntea (Hamilton): An Immuno-Histochemical study. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 83, 603–607 (2013). https://doi.org/10.1007/s40011-013-0163-0

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  • DOI: https://doi.org/10.1007/s40011-013-0163-0

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