Abstract
An attempt has been made to accelerate the flavour development in cheese slurry with the help of exogenous proteolytic and lipolytic enzymes (1:1 proportion, each at the rate of 0.025 % by weight of cheese-base) and ripening at elevated temperatures (i.e. 20 ± 1 °C) for up to 12 days. Bitterness, the necessary consequence of accelerated cheese ripening, has been the main objective of the present investigation. Adjunct cultures Lactobacillus casei and Lactobacillus helveticus were used as debittering aids. The debittering activity was monitored using electrophoretic pattern and sensory evaluation of the product. Freeze-shocked adjunct Lactobacillus helveticus produced enzyme-modified cheese slurry with no detectable bitterness. The usage of adjunct cultures either viable or attenuated, ripening period and interactions amongst these parameters had significant (P < 0.05) influence on all of the sensory characteristics monitored.
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References
Ziemer CJ, Gibson GR (1998) An overview of probiotcs, prebiotics and synbiotics in the functional food concept: perspectives and future strategies. Int Dairy J 8:473–479
de Souza CHB, Buriti FCA, Behrens JH, Saad SMI (2008) Sensory evaluation of probiotic Minas fresh cheese with Lactobacillus acidophilus added solely or in co-culture with a thermophillic starter culture. Int J Food Sci Technol 43:871–877
Smit LE, Schönfeldt HC, de Beer WHJ, Smith MF (2001) The influence of factory and region on the composition of South African Cheddar and Gouda Cheese. J Food Compd Anal 14:177–198
Kheadr EE, Vuillemard JC, El-Deeb SA (2003) Impact of liposome-encapsulated enzyme cocktails on cheddar cheese ripening. Food Res Int 36:241–252
Cogan TM, Beresford TP (2002) Microbiology of hard cheese. In: Robinson RK (ed) Dairy microbiology handbook, 3rd edn. Wiley, New York
Singh TK, Drake MA, Cadwallader KR (2003) Flavour of cheddar cheese: a chemical and sensory perspective comprehensive reviews. Food Sci Food Saf 2:139–162
Banks JM, Yvon M, Gripon JC, de la Fuente MA, Brechany EY, Williams AG, Muir DD (2001) Enhancement of amino acid catabolism in Cheddar cheese using α-ketoglutarate: amino acid degeneration in relation to volatile compounds and aroma character. Int Dairy J 11:235–243
Sudhir K, Jha YK, Pratibha S (2010) Influence of adjuncts as a debittering aids in encountering the bitterness developed in cheese slurry during accelerated ripening. Int J Food Sci Technol 45:1403–1409
Luckow T, Delahunty C (2004) Which juice is ‘healthier’? A consumer study of probiotic non-dairy juice drinks. Food Qual Pref 15:751–759
Urala N, Lahteenmaki L (2004) Attitudes behind consumers’s willingness to use functional food. Food Qual Pref 15:793–803
Sousa MJ, Ardö Y, McSweeney PLH (2001) Advances in the study of proteolysis during cheese ripening. Int Dairy J 11:327–345
Rajak J, Jha YK (2000) Effect of microbial enzymes, lactic acid bacteria and higher ripening temperatures on accelerated cheese flavour development in acid coagulated curd milk slurry. Bev Food World 27:33–36
AOAC (1975) Official methods of analysis, 12th edn. Association of Official Analytical Chemists, Washington, DC
Ezzat NI, El- Shafei HM (1991) Accelerated ripening of Ras cheese using freeze-shocked and heat-shocked Lactobacillus helveticus. Egypt J Dairy Sci 19:347–358
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Bhowmik T, Riesterer R, MAJS van Boekel, Marth EH (1990) Characteristics of low-fat Cheddar cheese made with added Micrococcus or Pediococcus species. Milchwissenschaft 45:230–235
Snedecor GW, Cochran WG (1968) Statistical methods, 6th edn. Iowa State University Press, Ames, p 593
El-Soda M, Madkor SA, Tong PS (2000) Evaluation of commercial adjuncts for use in cheese ripening: comparison between attenuated and not attenuated lactobacilli. Milchwissenschaft 55:260–293
El-Soda M, Madkor SA, Tong PS (2000) Adjuncts cultures: recent developments and potential significance to the cheese industry. J Dairy Sci 83:609–619
Katsiari MC, Voutsinas LP, Kondyli E (2002) Improvement of sensory quality of low-fat Kefalograviera-type cheese by using commercial special starter cultures. J Dairy Sci 85:2759–2767
Fox PF, Singh TK, McSweeney PLH (1995) Biogenesis of flavour compounds in cheese. In: Malin EL (ed) Chemistry of structure/function relationships in cheese. Plenum Press, New York, pp 59–98
Law BA (1984) Flavour development in cheese. In: Davies FL, Law BA (eds) Advances in the microbiology and biochemistry of cheese and fermented milk. Elsevier, Applied Science Publishers, London, pp 187–208
Brandsma RL, Mistry VV, Anderson DL, Baldwin KA (1994) Reduced fat Cheddar cheese from condensed milk. 3. Accelerated ripening. J Dairy Sci 77:897–906
Di Cagno R, De Pasquale I, De Angelis M, Gobbetti M (2012) Accelerated ripening of Caciocavallo Pugliese cheese with attenuated adjuncts of selected non-starter lactobacilli. J Dairy Sci 95(9):95–4784
Kim MS, Jenson LA, Olson NF (1987) Enhancing flavour characteristics of Gouda cheese from ultra filtered milk by addition of commercial enzyme preparations and freeze shocked Lactocillus helveticus. J Dairy Sci 70(Suppl. 1):66
Kim MS, Kim SC, Olson NF (1994) The effect of commercial fungal proteases and freeze-shocked Lactobacillus helveticus CRD 101 on accelerating cheese fermentation and proteolysis. Milchwissenschaft 49:442–445
Bartels HJ, Johnson ME, Olson NF (1987) Accelerated ripening of Gouda cheese. II. Effect of freeze-shocked Lactobacillus helveticus on proteolysis and flavour development. Milchwissenschaft 42:139–144
El-Soda M, Chen C, Riesterer B, Olson N (1991) Acceleration of low fat cheese ripening using lyophilized extracts of freeze-shocked cells of some cheese related microorganisms. Milchwissenschaft 46:358–360
Aly ME (1994) Flavour enhancement of low-fat Kasshkaval cheese using heat-shocked or freeze-shocked Lactobacillus delbrueckii var. helveticus cultures. Nahrung 38:501–510
Kebary KMK, Khader AE, Zedan AN, Mahmoud SF (1996) Accelerated ripening of low fat Ras cheese by attenuated lactobacilli cells. Food Res Int 29:705–713
Kebary KMK, Salem OM, Hamed AI, EI-Sisi AS (1997) Flavour enhancement of direct acidified kareish cheese using attenuated lactic acid bacteria. Food Res Int 30:265–272
Broome MC, Krause DA, Hickey MW (1990) The use of non starter lactobacilli in Cheddar cheese manufacture. Aust J Dairy Technol 45:67–73
Drake MA, Boylston TD, Spence KD, Swanson BG (1996) Chemical and sensory effects of Lactobacillus adjunct in Cheddar cheese. Food Res Int 29:381–387
Muir DD, Banks JM, Hunter EA (1996) Sensory properties of cheddar cheese: effect of starter type and adjunct. Int Dairy J 6:407–423
Akin MS (2012) Accelerated ripening of Kashar cheese with encapsulated protease. Afr J Biotechnol 11(66):13007–13015
Bartels HJ, Johnson ME, Olson NF (1987) Accelerated ripening of Gouda cheese. I. Effect of heat-shocked thermophillic lactobacilli and streptococci on proteolysis and flavour development. Milchwissenschaft 42:83
Frey JP, Johnson ME, Marth EH (1986) Peptidases and proteases in barley extract: a potential source of enzymes for use in cheese ripening. Milchwissesschaft 41:488
Lance RM (2011) Effect of adjunct cultures, sodium gluconate and ripening temperature on low fat cheese flavor. MSc Thesis submitted to Utah State University, Logan, Utah
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Kumar, S., Jha, Y.K. & Singh, P. Influence of Adjuncts as Debittering Aids on the Sensory and Biochemical Properties of Enzyme Modified Cheese-Base. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 83, 347–355 (2013). https://doi.org/10.1007/s40011-012-0146-6
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DOI: https://doi.org/10.1007/s40011-012-0146-6