Some of the plant derived bioactives are called as ‘natural antioxidants’ for their role in protecting the cells from injurious effect of reactive oxygen species. The study investigated the concentration of natural bioactives and antioxidant capacity in Eryngiumfoetidum L. leaves. The antioxidant activity was determined in leaf extracts prepared in five solvents (methanol, acetone, petroleum ether, chloroform and water). The concentration of bioactive compounds (polyphenol, tannin, anthocyanin, flavonoids, carotenoids and ascorbic acid) varied in the extracts prepared with different solvents. The highest recovery of these bioactive compounds was observed with acetone and methanol. The contents of anti-nutritional factors, namely saponin, nitrate, phytate and oxalate content were also estimated in the leaf extracts. HPLC analysis of methanol leaf extract led to detection of several carotenoids (lutein, zeaxanthin, β-cryptoxanthin, β-carotene, chlorophyll-a, chlorophyll-b and pheophytin-b), phenolics (gallic acid, protocatechuic acid, syringic acid, p-coumaric acid, ferulic acid and sinapic acid) and anthroquinones (norlichexanthone, telochistin, secalonic acid D, citreorosein, emodin and parietin). Present study has revealed antioxidant potential of E. foetidum for possible use by food and pharmaceutical industry.
Eryngium foetidum L. Phytochemicals Antioxidant activity Solvents Anti-nutrients HPLC analysis
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The authors are grateful to the Director, Central Agricultural Research Institute, Port Blair for providing facilities to conduct experiments and also acknowledge the settlers and Nicobari tribe for their responses to the survey.
Pratt DE (1992) Natural antioxidants from plant material. In: Huang IMT, Ho CT, Lee CY (eds) Phenolic compounds in food and their effects on health. American Chemical Society, New York, pp 54–72CrossRefGoogle Scholar
Shon MY, Kim TH, Sung NJ (2003) Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chem 82:593–597CrossRefGoogle Scholar
Singh S, Singh DR, Salim KM, Srivastava A, Singh LB, Srivastava RC (2011) Estimation of proximate composition, micronutrients and phytochemical compounds in traditional vegetables from Andaman & Nicobar Islands. Int J Food Sci Nutr 62:765–773PubMedCrossRefGoogle Scholar
Zainol MK, Abd-Hamid A, Yusof S, Muse R (2003) Antioxidant activity and total phenolic compounds of leaf, root and petiole of four accessions of Centellaasiatica (L.) urban. Food Chem 81:575–591CrossRefGoogle Scholar
Sáenz MT, Fernadez MA, Garcia MD (1997) Antiinflammatory and analgesic properties from leaves of Eryngium foetidum L. (Apiaceae). Phytother Res 11:380–383CrossRefGoogle Scholar
Djeridane A, Yousli M, Nadjemi B, Boutassouna D, Stocker P, Vidal N (2006) Antioxidant activity of some Algerian medicinal plant extracts containing phenolic compounds. Food Chem 97:654–660CrossRefGoogle Scholar
Rao EVSP, Puttanna K (2000) Nitrates agriculture and environment. Curr Sci (India) 79:1163–1168Google Scholar
Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods Enzymol 299:152–178CrossRefGoogle Scholar
Lichtenthaler HK, Buschmann (2001) Chlorophylls and carotenoids: measurement and characterization by UV–Vis spectroscopy. In: Wrolstad RE (ed) Current protocols food analytical chemistry. Wiley, New York, pp F4.3.1–F4.3.8Google Scholar
AOAC (1995) Official methods of analysis. Association of official analytical chemists, 16th edn. American Public Health Association, WashingtonGoogle Scholar
Wong CC, Li HB, Cheng KW, Chen F (2006) A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem 97:705–711CrossRefGoogle Scholar
Hassan LG, Dangoggo SM, Umar KJ, Saidu I, Folorunsho FA (2008) Proximate, minerals and anti-nutritional factors of Daniellia oliveri seed kernel. Chem Class J 5:31–36Google Scholar
Olives Barba AL, Huratado CM, Mata SMC, Ruiz FV, Lopez STM (2006) Application of a UV-Vis detection-HPLC method for a rapid determination of lycopene and beta carotene in vegetables. Food Chem 95:328–336CrossRefGoogle Scholar
Ahmad MN, Saleemullah M, Shah H, Khalil IA, Saljoqui AUR (2007) Determination of beta-carotene content in fresh vegetables using high performance liquid chromatography. Sarhad J Agric 23:767–770Google Scholar
Shotipruk A, Kiatsongserm J, Pavasant P, Goto M, Sasaki M (2004) Pressurized hot water extraction of anthraquinones from the roots of Morinda citrifolia. Biotechnol Prog 20:1872–1875PubMedCrossRefGoogle Scholar
Manojlovic TN, Solujic S, Sukdolak S (2002) Antimicrobial activity of an extract and anthraquinones from Caloplaca schaereri. Lichenologist 34:83–85CrossRefGoogle Scholar
Smith MA, Perry G, Richey PL, Sayre LM, Anderson V, Beal MF, Kowal N (1996) Oxidative damage in Alzheimer’s. Nature 382:120–121PubMedCrossRefGoogle Scholar