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RAPD Analysis to Study Metagenome Diversity in Soil Microbial Community of Arid Zone Plants

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Abstract

The study was conducted by taking six soil samples, two each for Capparis decidua (Ker), Calligonum polygonoides (Phog) collected from Bikaner while third sample, Cuminum cyminum (Cumin) was collected from Jobner. RAPD analysis was done using various decamer primers. The total bands amplified varied from 7 (UBC 153) to 13 (UBC 157). Three primers (UBC 153, 154 and 162) showed 100 % polymorphism. 46 out of 53 polymorphic allelic positions were obtained with 86.7 % polymorphism. The number of bands generated was plant and soil dependent.

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Authors and Affiliations

  1. Department of Life Sciences, Jaipur National University, Jaipur, Rajasthan, India

    G. Sharma & H. N. Verma

  2. Department of Plant Biotechnology, Rajasthan Agriculture University, Bikaner, Rajasthan, India

    R. Sharma

Authors
  1. G. Sharma
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  2. H. N. Verma
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  3. R. Sharma
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Correspondence to H. N. Verma.

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Sharma, G., Verma, H.N. & Sharma, R. RAPD Analysis to Study Metagenome Diversity in Soil Microbial Community of Arid Zone Plants. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 83, 135–139 (2013). https://doi.org/10.1007/s40011-012-0041-1

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  • DOI: https://doi.org/10.1007/s40011-012-0041-1

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