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Proton NMR and Chromatography-Based Phytochemical Analysis of Cajanus cajan Pods

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Abstract

The present research work was aimed towards the preparation of different of C. cajan (Fabaceae) pods; their phytochemical analysis by 1H-NMR, HPTLC and hyphenated techniques like HR-LC/MS and GC-MS. Based on results of preliminary phytochemical studies, petroleum ether and ethanolic extracts were selected for further 1H-NMR, HPTLC and HR-LC/MS and GC-MS analysis. After preliminary detection of phytochemicals, ethanolic and petroleum ether extracts of C. cajan pods tested for distribution of hydrogens in phytochemicals using 1H-NMR. Then, HPTLC profiling of both extracts confirmed the classes of phytochemicals. Further, HR-LC/MS analysis of ethanolic extract carried out at both positive and negative ion mode, indicated the presence of more than 70 compounds, many of which, as per METLIN database, predicted as phytochemicals were different types of flavonoids and other iridoids and terpenoids. GC-MS analysis of petroleum ether extract showed various non-polar compounds, which were predicted as simple hydrocarbons. Previous reports revealed that most of these predicted compounds are biologically active. C. cajan pods contain different phytochemicals like flavonoids, iridoids, steroids, both in free and glycosidic forms and also non-polar hydrocarbons; appearance of all these compounds is significant in chemotaxonomic surveillance of this plant.

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Acknowledgements

We, the authors of this research article are thankful to NMR facility, Central Instrumentation Facility (CIF), Department of Chemistry, Savitribai Phule Pune University, Pune; Graduate School of Pharmacy, Gujarat Technological University, Gandhinagar; Sophisticated Analytical Instrument Facility (SAIF)—Indian Institute of Technology-Bombay, Mumbai for providing Proton NMR, HPTLC, and HR-LC/MS and GC-MS facilities, respectively.

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  1. TVES’s Hon.ble Loksevak Madhukarrao Chaudhari College of Pharmacy, Faizpur, 425 503, Tal-Yawal, Dist-Jalgaon, Maharashtra, India

    Shriniwas P. Patil, Rajesh Y. Chaudhari & Mahesh S. Nemade

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  1. Shriniwas P. Patil
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  2. Rajesh Y. Chaudhari
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Correspondence to Shriniwas P. Patil.

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Patil, S.P., Chaudhari, R.Y. & Nemade, M.S. Proton NMR and Chromatography-Based Phytochemical Analysis of Cajanus cajan Pods. Natl. Acad. Sci. Lett. 47, 139–145 (2024). https://doi.org/10.1007/s40009-023-01329-5

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  • DOI: https://doi.org/10.1007/s40009-023-01329-5

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