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Morpho-Molecular Identification of Lasiodiplodia theobromae Causing Root Rot of Mulberry in India


The root rot disease caused by Lasiodiplodia theobromae in Morus alba emerged as a serious problem that affect sustainable mulberry production. In this study, the disease symptoms like yellowing of leaves, blackening, and rotting of roots were observed. The associated pathogen was isolated from infected root tissue and pure culture was raised. Based on morphological features, ITS sequencing, and phylogenetic analyses, the fungal pathogen is identified as Lasiodiplodia theobromae. The sequence was deposited in NCBI with the accession number MK811183. The pathogenicity was confirmed by artificial inoculation on mulberry seedlings. This disease can cause great economic loss, as every part of the plant is infected. So, correct identification of disease to take timely management measures is a must, this basic work will give proper identification of pathogen and help to improvise the mulberry production in the country.

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Significance Statement This work is carried out to find the causal organism of mulberry root rot disease. Here, symptoms caused by the pathogen on host plant, its morphological character and molecular identification reveals that Lasiodiplodia theobromae is the causal organism. Further, pathogenicity test also validates the above prediction.

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Kanyakumari, R., Somasekhara, Y.M., Basavaraj, S. et al. Morpho-Molecular Identification of Lasiodiplodia theobromae Causing Root Rot of Mulberry in India. Natl. Acad. Sci. Lett. 45, 451–456 (2022).

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  • Root rot
  • Disease
  • Mulberry
  • Bombyx mori
  • Identification