Advertisement

National Academy Science Letters

, Volume 42, Issue 3, pp 259–263 | Cite as

In Vitro Antagonism of Trichoderma Isolates Against Curvularia andropogonis Causing Leaf Blight of Java Citronella

  • Shailesh PandeyEmail author
  • Rajesh Kumar
  • Krishna Giri
Short Communication
  • 39 Downloads

Abstract

Leaf blight is the most devastating disease of Java Citronella (Cymbopogon winterianus) in North east India. The pathogen was isolated and identified as Curvularia andropogonis. A total of 6 Trichodema isolates were recovered from forest soil and tested against the target pathogen. Analysis of variance (ANOVA) revealed significant differences among Trichoderma isolates in checking the mycelial growth of C. andropogonis in dual culture, inverted plate and culture filtrate assay (P < 0.05). Two potential Trichoderma isolates were identified as T. asperellum and T. virens using sequence analysis of internal transcribed spacer region of the ribosomal DNA. Both the species were found more effective and significantly inhibited the growth of the test pathogen in vitro. Our results suggest appealing application possibilities of Trichoderma isolates in the biological management of leaf blight disease of Java Citronella.

Keywords

Curvularia andropogonis Trichoderma virens Trichoderma asperellum Cymbopogon winterianus Biological Control 

Notes

Acknowledgement

The support by the Indian Council of Forestry Research and Education, Dehradun, for funding the Research Project ‘RFRI/2013-14/FP-5′ is gratefully acknowledged. Authors also express our gratitude to National Fungal Culture Collection of India (NFCCI), Pune for confirming the identities of fungal isolates.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Mao AA, Hynniewta TM, Sangappa M (2009) Plant wealth of North east India with reference to Ethnobotany. Indian J Tradit Knowl 8(1):96–103Google Scholar
  2. 2.
    Ganjewala D (2009) Cymbopogon essential oils: chemical compositions and bioactivities. Int J Essent Oil Ther 3:56–65Google Scholar
  3. 3.
    Chutia M, Mahanta JJ, Saikia RC, Baruah AKS, Sharma TC (2006) Influence of Leaf Blight disease on yield and its constituents of Java Citronella and in vitro control of the pathogen using essentials oil. World J Agric Sci 2(3):319–321Google Scholar
  4. 4.
    Yang C, Hamel C, Vujanovic V, Gan Y (2011) Fungicide: modes of Action and Possible Impact on Nontarget Microorganisms. ISRN Ecology.  https://doi.org/10.5402/2011/130289 Google Scholar
  5. 5.
    Subramanian CV (1953) Fungi Imperfecti from Madras. V. Curvularia. Proc Indian Acad Sci Sect B 3:27–39Google Scholar
  6. 6.
    Gil SV, Pastorb S, Marcha GJ (2009) Quantitative isolation of biocontrol agents Trichoderma spp., Gliocladium spp. and actinomycetes from soil with culture media. Microbiol Res 164(2):196–205CrossRefGoogle Scholar
  7. 7.
    Morton DJ, Stroube WH (1955) Antagonistic and stimulating effects of soil micro-organism upon Sclerotium rolfsii. Phytopathology 45:417–420Google Scholar
  8. 8.
    Dennis C, Webster J (1971) Antagonistic properties of species groups of Trichoderma- II. Production of volatile antibiotics. Trans Br Mycol Soc 57:47–48Google Scholar
  9. 9.
    Grover RK, Moore JD (1962) Toximetric studies of fungicides against brown rot organism. Sclerotina fruticola. Phytopathology 52:876–880Google Scholar
  10. 10.
    Anees M, Tronsmo A, HermannVE Hjeljord LG, Heraud C, Steinberg C (2010) Characterization of field isolates of Trichoderma antagonistic against Rhizoctonia solani. Fungal Biol 114:691–701CrossRefGoogle Scholar
  11. 11.
    Diaz G, Corcoles AI, Asencio AD, Torres MP (2012) In vitro antagonism of Trichoderma and naturally occurring fungi from elms against Ophiostoma novo-ulmi. For Pathol 43(1):51–58Google Scholar
  12. 12.
    Tapwal A, Singh U, Teixeira da Silva JA, Singh G, Garg S, Kumar R (2011) In vitro antagonisn of Trichoderma viride against five phytopathogens. Pest Technology 5(1):59–62Google Scholar
  13. 13.
    Muthukumar A, Eswaran A, Sanjeevkumas K (2011) Exploitation of Trichoderma species on the growth of Pythium aphanidermatum in Chilli. Braz J Microbiol 42(4):1598–1607CrossRefGoogle Scholar
  14. 14.
    Sharfuddin C, Mohanka R (2012) In vitro antagonism of indigenous Trichoderma isolates against phytopathogen causing wilt of lentil. Int J Life Sci Pharma Res 2(3):195–202Google Scholar
  15. 15.
    Rahman MA, Begum MF, Alam MF (2009) Screening of Trichoderma isolates as a biological control agent against Ceratocystis paradoxa causing Pineapple Disease of Sugarcane. Mycobiology 37(4):277–285CrossRefGoogle Scholar
  16. 16.
    Dennis C, Webster J (1971) Antagonistic properties of species groups of Trichoderma—II. Production of volatile antibiotics. Trans Br Mycol Soc 57:47–48Google Scholar
  17. 17.
    Schirmbock M, Lorito M, Wang YL, Hayes CK, Arisan AI, Scala F, Herman GE, Kubicek CP (1994) Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi. Appl Environ Microbiol 60(12):4344–4370Google Scholar
  18. 18.
    Kucuk C, Kivanc M (2004) In vitro antifungal activity of strains of Trichoderma harzianum. Turk J Biol 28:111–115Google Scholar
  19. 19.
    Reino JL, Guerrero RF, Galan RH, Collado IJ (2008) Secondary metabolites from species of the biocontrol agent Trichoderma. Phytochem Rev 7:89–123CrossRefGoogle Scholar
  20. 20.
    Vinale F, Sivasithamparam K, Ghisalberti EL, Marra R, Barbetti MJ, Li H, Woo SL, Lorito M (2008) A novel role for Trichoderma secondary metabolites in the interactions with plants. Physiol Mol Plant Pathol 72:80–86CrossRefGoogle Scholar
  21. 21.
    Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma species—opportunistic, avirulent plant symbionts. Nat Rev Microbiol 2:43–56CrossRefGoogle Scholar
  22. 22.
    Druzhinina IS, Kopchinskiy AG, Kubicek CP (2006) The first 100 Trichoderma species characterized by molecular data. Mycoscience 47:55–64CrossRefGoogle Scholar
  23. 23.
    Latha J, Verma A, Mukherjee PK (2002) PCR-fingerprinting of some Trichoderma isolates from two Indian type culture collections—a need for re-identification of these economically important fungi. Curr Sci 83(4):372–374Google Scholar
  24. 24.
    Mukherjee PK, Mukherjee AK, Krathi S (2013) Reclassification of Trichoderma viride (TNAU), the Most Widely Used Commercial Biofungicide in India, as Trichoderma asperelloides. Open Biotechnol J 7:7–9CrossRefGoogle Scholar
  25. 25.
    Druzhinina IS, Komon-Zelazowska M, Kredics L, Hatvani L, Antal Z, Belayneh T, Kubicek CP (2008) Alternative reproductive strategies of Hypocrea orientalis and genetically close but clonal Trichoderma longibrachiatum, both capable of causing invasive mycoses of humans. Microbiology 154:3447–3459CrossRefGoogle Scholar
  26. 26.
    Kuhls K, Lieckfeldt E, Borner T, Gueho E (1999) Molecular reidentification of human pathogenic Trichoderma isolates as Trichoderma longibrachiatum and Trichoderma citrinoviride. Med Mycol 37:25–33CrossRefGoogle Scholar
  27. 27.
    Samuels GJ, Dodd SL, Gams W, Castlebury LA, Petrini O (2002) Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia 94:146–170CrossRefGoogle Scholar
  28. 28.
    Błaszczyk L, Siwulski M, Sobieralski K, Frużynska-Jozwiak D (2013) Diversity of Trichoderma spp. causing Pleurotus green mould diseases in Central Europe. Folia Microbiol 58(4):325–333CrossRefGoogle Scholar
  29. 29.
    Weindling R (1932) Trichoderma lignorum as a parasite of other soil fungi. Phytopathology 22:837–845Google Scholar
  30. 30.
    Benitez T, Rincon AM, Limón MC, Codón AC (2004) Biocontrol mechanisms of Trichoderma strains. Int Microbiol 7:249–260Google Scholar
  31. 31.
    Howell CR (2003) Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis 87:4–10CrossRefGoogle Scholar
  32. 32.
    Harman GE (2011) Multifunctional fungal plant symbionts: new tools to enhance plant growth and productivity. New Phytol 189:647–649CrossRefGoogle Scholar
  33. 33.
    Sarma A, Saikia RC, Sarma TC (2004) Effect of leaf blight disease on citronella oil and its major constituents and in vitro control of disease through application of bio-control agent. J Essent Oil Bear Plants 7(3):288–292CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.Rain Forest Research InstituteJorhatIndia
  2. 2.Forest Protection DivisionForest Research InstituteDehradunIndia

Personalised recommendations