Abstract
Shikimic acid (SA) is a key precursor for synthesis of drug against H5N1 virus. The demand for this compound is met from plant resources, especially from the fruits of Chinese star anise (Ilichium vernum L.). Present study expounds understanding on distribution of SA in various plant species collected from Western Ghats using RP-UFLC analysis. The area under curve obtained after RP-UFLC run were used to comprehend on possible natural groupings and correlations. 14 out of 58 samples yielded SA in an excess of 1 mg/g and Mammea suriga (Buch.-Ham. ex Roxb.) Kosterm (29.34 mg/g) being the highest among all. Probably, this is the first report of M. suriga to be accounted for SA content. Higher yielding samples grouped together in hierarchical clustering analysis.
References
Wang XQ, Guo YJ, Yang CS (2001) Determination of shikimic acid in fruit of Illiciaceae plants by HPLC with diode-array detection. Zhongguo Zhong Yao Za Zhi 26(7):447–449
Hollander H, Amrhein N (1980) The site of the inhibition of the shikimiate pathway by glyphosate. Plant Physiol 66:823–829
Harring T, Streibig JC, Husted S (1998) Accumulation of shikimic acid: a technique for screening glyphosate efficacy. J Agric Food Chem 46:4406–4412
Frost JW, Frost KM, Knop DR (2003) Biocatalytic synthesis of Shikimic acid. US Patent 6,613,552 B1
Kramer M, Bongaerts J, Bovenberg R, Kremer S, Muller S, Orf S et al (2003) Metabolic engineering for microbial production of shikimic acid. Metabolic Eng 5:277–283
Shaner DL, Nadler-Hassar T, Henry WB, Koger CH (2005) A rapid in vivo shikimate accumulation assay with excised leaf discs. Weed Sci 53:769–774
Edmonds M, Payne R (2005) Isolation of shikimic acid from star aniseed. J Chem Educ 82(4):599
Raghavendra TR, Vaidyantahan P, Swathi HK, Ramesha BT, Ganeshaiah KN, Srikrishna A et al (2009) Prospecting for alternate sources of shikimic acid, a precursor of Tamiflu, a bird flu drug. Curr Sci 96(6):771–772
Escalante A et al (2010) Engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system. Micob Cell Fact 9:21. https://doi.org/10.1186/1475-2859-9-21
Hulme AC (1956) Shikimic acid in apple fruits. Nature 178:991–992
Hillis WE (1959) Shikimic acid in the leaves of Eucalyptus sieberiana F. Muell J Exp Bot 10(1):87–89
Sui R (2008) Separation of shikimic acid from pine needles. Chem Eng Technol 31(3):469–473
Yoshida S, Tazaki K, Minamikawa T (1975) Occurrence of shikimic and quinic acids in angiosperms. Phytochemistry 14(1):195–197
Enrich Liza B, Scheuermann Margaret L, Mohadjer A et al (2008) Liquidambar styraciflua: a renewable source of shikimic acid. Tetrahedron Lett 49:2503–2505
Simonart P, Wiaux A (1960) Production of shikimic acid by Penicillium griseofulvum Dierckx. Nature 186:78–79
Ghosh S, Chisti Y, Banerjee UC (2012) Production of shikimic acid. Biotech Adv 30(6):1425–1431
Lydon J, Duke OS (1988) Glyphosate induction of elevated levels of hydroxybenzoic acids in higher plants. J Agric Food Chem 36:813–818
Amrhein N, Deus B, Gehrke P, Steinrucken HC (1980) The site of the inhibition of the shikimate pathway by glyphosate: II. Interference of glyphosate with chorismate formation in vivo and in vitro. Plant Physiol 66:830–834
Berlin J, Witte L (1981) Effects of glyphosate on shikimic acid accumulation in tobacco cell cultures with low and high yields of cinnamoyl putrescines. Zeitschrift für Naturforschung C 36:210–214
Acknowledgements
Authors are thankful to Director-in-Charge, RMRC, ICMR, Belagavi for providing the necessary facilities. Authors also thank Dr. Vinayak Upadhya for his extended help during collection. Thanks to Mr. Venkatesh and Mr. Bhoopal for laboratory assistance. This study was funded by Indian Council of Medical Research, New Delhi (Grant Number: 59/42/2012/online/BMS/TRM).
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Kshirsagr, P.R., Pai, S.R. & Hegde, H.V. Comparison of Shikimic Acid Content: A Precursor for Drug Against H5N1 from Various Plant Species of Western Ghats, India. Natl. Acad. Sci. Lett. 41, 399–402 (2018). https://doi.org/10.1007/s40009-018-0687-1
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DOI: https://doi.org/10.1007/s40009-018-0687-1