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National Academy Science Letters

, Volume 42, Issue 2, pp 179–184 | Cite as

Seasonal Diversity of Microfungi in Rhizosphere of Dalbergia sissoo in New Forest, Campus, Dehradun (India)

  • Pallavi BhatiaEmail author
  • R. C. Dubey
  • N. S. K. Harsh
  • P. K. Kaushik
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Abstract

Rhizosphere microfungi were isolated by using serial dilution plate method. The highest fungal population was recorded in rainy season as compared to other seasons. Thirty-six fungal species belonging to fifteen genera were recovered throughout the seasons with the highest population and relative abundance of Cuninghamella burtholletiae (8.4%) followed by Mucor varians (7.5%) and Penicillium frequentens (7.2%) and lowest by Cladosporium cladosporioides and sterile mycelia I (0.5% each). The members of ascomycetes (66.67%) were dominant in the study site followed by that of Phycomycetes (20%), Basidiomycetes (6.67%) and sterile mycelia (6.67%). The highest fungal diversity index was found in summer (3.33), followed by rainy (3.17) and winter (2.98) seasons. The greatest similarity index in soil fungi occurred between summer and rainy (88.52) seasons followed by winter and rainy (76.92) and winter and summer (72.72). Significant positive correlations were observed between moisture and available nitrogen with fungal population in winter season, while soil temperature, moisture, organic matter, organic carbon, available nitrogen and available potassium showed positive correlations with fungal population in summer and winter. Isolated saprophytic fungi were also found dominant over the pathogenic isolates of the specie due to their antagonistic behavior against the pathogens.

Keywords

Antagonistic property Colony forming unit Diversity index Relative abundance Rhizosphere fungi Similarity index 

References

  1. 1.
    Finlay RD (2007) The fungi in soil. In: Van Elsas JD, Jansson JK, Trevors JT (eds) Modern soil microbiology, 2nd edn. CRC Press, New York, pp 107–146Google Scholar
  2. 2.
    Sarbhoy AK, Agarwal DK, Varshney JL (1996) Fungi of India. CBS Publishers and Distributors, New DelhiGoogle Scholar
  3. 3.
    Dubey RC, Maheshwari DK (2011) Cultivation and isolation of microorganisms. Practical Microbiology, 2nd edn. S. Chand and Co, New Delhi, pp 78–109Google Scholar
  4. 4.
    Barnett HL, Hunter BB (1972) Illustrated genera of imperfect fungi. Burgess Publication, MinneapolisGoogle Scholar
  5. 5.
    Bissett J (1992) Trichoderma atroviride. Can J Bot 70:691–693CrossRefGoogle Scholar
  6. 6.
    Booth C (1971) The genus Fusarium. Commonwealth Mycological Institute, KewGoogle Scholar
  7. 7.
    Gilman JC (1957) A manual of soil fungi. Iowa State College Press, AmesCrossRefGoogle Scholar
  8. 8.
    Stalpers JA (1978) Identification of wood inhibiting aphyllophorales in pure culture. Central Bureau Voor Schimmel Culture. Stud Mycol 1:248Google Scholar
  9. 9.
    Sen M (1973) Cultural diagnosis of Indian polyporaceae. Indian Forest Records, Forest Research Institute, DehradunGoogle Scholar
  10. 10.
    Walkley A, Black IA (1934) An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38ADSCrossRefGoogle Scholar
  11. 11.
    Rodrigues BF, Jaiswal V (2008) Effect of distribution on arbuscular mycorrihzal (AM) fungal population in coastal sand dune vegetation of Goa. India J Mycol Plant Pathol 38(1):73–79Google Scholar
  12. 12.
    Shannon CE, Weiner W (1963) The mathematical theory of communication. University of Illinois Press, ChampaignGoogle Scholar
  13. 13.
    Sorrenson T (1948) A method of establishing groups of equal amplitude in plant sociology based on similarity of species. Cotent-Det. Korgelige danske Videnskabernes Selstab. Biol Skr Bind 5:1–34Google Scholar
  14. 14.
    Skidmore AM, Dickinson CH (1976) Colony interaction and hyphal interference between Septoria nodorum and phylloplane fungi. Trans Br Mycol Soc 66:57–64CrossRefGoogle Scholar
  15. 15.
    Dwivedi RS (1966) Ecology of soil fungi of some grasslands of Varanasi-II, Distribution of soil mycoflora. Bull Int Soc Trop Ecol 7:84–99Google Scholar
  16. 16.
    Persiani AG, Maggi MA, Pineda FDC (1998) Diversity and variability in soil fungi from a distributed tropical rain fores. Mycologia 90(2):206–214CrossRefGoogle Scholar
  17. 17.
    Rama Rao P (1969) Studies on soil fungi-III, seasonal variation and distribution of microfungi in some soils of Andhra Pradesh. Trans of Br Mycol Soc 52(2):277–298Google Scholar
  18. 18.
    Dkhar MS, Mishra R (1987) Microbial population, fungal biomass and CO2 evolution in maize (Zea mays) field soils. Plant Soil 99:277–283CrossRefGoogle Scholar
  19. 19.
    Entry JA, Emmingham WH (1996) Influence of vegetation on microbial degradation of atrazine and 2, 4-dichlorophenoxyacetic acid in riparian soils. Can J Soil Sci 76:101–106CrossRefGoogle Scholar
  20. 20.
    Tsai SH, Selvam A, Yang SS (2007) Microbial diversity of topographical gradient profiles in Fushan Forest soils of Taiwan. Ecol Res 22:814–824CrossRefGoogle Scholar
  21. 21.
    Jha DK, Sharma GD, Mishra RR (1992) Ecology of soil microflora and mycorrhizal symbionts in degraded forests at two attitudes. Biol Fertil Soils 12:272–278CrossRefGoogle Scholar
  22. 22.
    Schimel J (1995) Ecosystem consequences of microbial diversity and community structure. In: Korner C (ed) Arctic and alpine biodiversity—patterns, causes and ecosystem consequences. Ecological Studies, Heidelberg, pp 239–254Google Scholar
  23. 23.
    Bossio DA, Girvan MS, Verchot L, Bullimore J, Borelli T, Albrecht A, Scow KM, Ball AS, Pretty JN, Osborn AM (2005) Soil microbial community response to land use change in an agricultural landscape of Western Kenya. Microb Ecol 49:50–62CrossRefGoogle Scholar
  24. 24.
    Kennedy NM, Gleeson DE, Connoly J, Clipson NJW (2005) Seasonal and management influences on bacterial community structure in an upland grassland soil FEMS. Microbiol Ecol 53:329–337CrossRefGoogle Scholar
  25. 25.
    Schreven DA (1967) The effect of intermittent drying and wetting of a calcareous soil on carbon and nitrogen mineralization. Plant Soil 26:14–32CrossRefGoogle Scholar
  26. 26.
    Tiwary CK, Pandey U, Singh JS (1982) Soil and litter respiration rates in different microhabitats of a mixed oak-conifer forest and their control by edaphic conditions and substrate quality. Plant Soil 65:233–238CrossRefGoogle Scholar
  27. 27.
    Majdah Al-Tuwaijri (2009) Role of the biocontrol agents Trichoderma viride and Bacillus subtilis in elimination of the deteriorative effects of the root-rot pathogens Fusarium oxysporum and F. solani on some metabolic and enzyme activities of cucumber plants. Egypt J Exp Biol 5:29–35Google Scholar
  28. 28.
    Diallo S, Crepin A, Barbey C, Orange N, Burini JF, Latour X (2011) Mechanisms and recent advances in biological control mediated through the potato rhizosphere. FEMS Microbiol Ecol 75(3):351–364CrossRefGoogle Scholar
  29. 29.
    Belete E, Ayalew A, Ahmed S (2015) Evaluation of local isolates of Trichoderma spp. against black root rot (Fusarium solani) on faba bean. Plant Pathol Microbiol 6(6):1–5Google Scholar
  30. 30.
    Larkin RP, Fravel DR (1998) Efficacy of various fungal and bacterial biocontrol organisms for control of Fusarium wilt of tomato. Plant Dis 82:1022–1028CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • Pallavi Bhatia
    • 1
    Email author
  • R. C. Dubey
    • 1
  • N. S. K. Harsh
    • 2
  • P. K. Kaushik
    • 3
  1. 1.Department of Botany and MicrobiologyGurukula Kangri VishwavidyalayaHaridwarIndia
  2. 2.Forest Pathology DivisionForest Research InstituteDehradunIndia
  3. 3.Chemistry DivisionForest Research InstituteDehradunIndia

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