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Optimized Densitometric Analysis for Determination of Triterpenoid Isomers in Vitex negundo L. Leaf

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Abstract

Triterpenoid isomers oleanolic acid (OA) and betulinic acid (BA) were densitometrically separated and quantified using an optimized high performance thin layer chromatography analysis in Vitex negundo. Multiple wavelengths (400–675 nm at 25 nm increment) were scanned during the densitometric analysis, and 600 nm was found optimum to quantify OA and BA simultaneously. This technique allowed optimum separation of OA and BA with distinguishing band color and corresponding Rf than the previous reports. The leaf extract contained higher amount of BA (56.74 ± 2.83 ng/band) compared to OA (16.25 ± 0.81 ng/band). The method provides better separation, resolution at optimized wavelength for these compounds at lower concentration levels.

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Acknowledgements

The authors are thankful to Indian Council of Medical Research (ICMR), New Delhi, India for necessary support. SRP is also indebted to Science and Engineering Research Board, Department of Science and Technology, New Delhi, for financial support (Grant No. SB/YS/LS-71/2013) during the work.

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  1. Sandeep R. Pai

    Present address: Amity Institute of Biotechnology, Amity University, Mumbai - Pune Expressway, Bhatan, Post – Somatane, Panvel, Mumbai, Maharashtra, 410206, India

Authors and Affiliations

  1. Department of Plant Biotechnology and Tissue Culture, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belagavi, Karnataka, 590 010, India

    Sandeep R. Pai

  2. Department of Phytochemistry, Regional Medical Research Centre, Indian Council of Medical Research, Nehru Nagar, Belagavi, Karnataka, 590 010, India

    Rajesh K. Joshi

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Correspondence to Rajesh K. Joshi.

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Pai, S.R., Joshi, R.K. Optimized Densitometric Analysis for Determination of Triterpenoid Isomers in Vitex negundo L. Leaf. Natl. Acad. Sci. Lett. 41, 323–327 (2018). https://doi.org/10.1007/s40009-018-0680-8

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  • DOI: https://doi.org/10.1007/s40009-018-0680-8

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