Skip to main content
SpringerLink
Log in

Differential Expression of Middle Silk Gland Proteins Caused by Cold Stress in Philosamia ricini

  • Short Communication
  • Published:
National Academy Science Letters Aims and scope Submit manuscript

Abstract

This study reports differentially expressed proteins in the middle silkglands of normal and cold stress treated 5th instar Philosamia ricini larvae by using high resolution two-dimensional polyacrylamide gel electrophoresis. About 300 protein spots were resolved in both type of the larvae. Most of the proteins were shown to be distributed in the area from 20 to 70 kDa ranging between pH 4 and 8. Six proteins showed considerably decreased expression in cold stress treated samples. Three proteins displayed increased level of expression after cold stress treatment, while two other proteins were expressed exclusively in cold stress treated silkgland tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Matsumara, Ihizuka Y (1929) The effect of temperature on development of Bombyx mori L. Represent Nagano Seric Exp Stn Jpn 19:1929

    Google Scholar 

  2. Wu DJ, Hou RF (1993) The relationship between thermo tolerancy and heat stable esterase in the silkworm Bombyx mori L., (Lepidoptera: Bombycidae). Appl Entomol Zool 28:371–377

    Google Scholar 

  3. Thiagarajan V, Bhargava SK, Ramesh babu M, Nagaraj B (1993) Differences in seasonal performance of twenty-six strains of silkworm, Bombyx mori (Bombycidae). J Lepid Soc 47:331–337

    Google Scholar 

  4. Reddy PL, Naik SS, Reddy NS (2002) Implications of temperature and humidity on the adult eclosion patterns in silkworm Bombyx mori L. J Entomol Res Soc 26:223–228

    Google Scholar 

  5. Watanabe H (1960) Temperature effects on the heterosis of starvation resistance in the silkworm, Bombyx mori. J Seric Sci Jpn 29:59–62

    Google Scholar 

  6. Ueda S, Lizuka H (1962) Studies on the effects of rearing temperature affecting the health of silkworm larvae and upon the quality of cocoons-1. Effect of temperature in each instar. Acta Sericol Jpn 41:6–21

    Google Scholar 

  7. Shirota T (1992) Selection of healthy silkworm strains through high temperature rearing of fifth instar larvae. Rep Silk Sci Res Inst 40:33–40

    Google Scholar 

  8. Rahmathulla VK (2012) Management of climatic factors for successful silkworm (Bombyx mori L.) crop and higher silk production: a review. Psyche. doi:10.1155/2012/121234

    Google Scholar 

  9. Pant R, Gupta DK (1979) The effect of exposure to low temperature on the metabo lism of carbohydrates, lipids and protein in the larvae of Philosamia ricini. J Biosci 1:441–446

    Article  Google Scholar 

  10. O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    Google Scholar 

  11. Rabilloud TH, Valette C, Lawrence JJ (1994) Sample application by in-gel rehydration improves the resolution of two dimensional electrophoresis with immobilized pH gradients in the first dimension. Electrophoresis 15:1522–1558

    Google Scholar 

  12. Sanchez JC, Rouge V, Pisteur M, Ravier F, Tonella L, Moosmayer M, Wilkins MR, Hochstrasser DF (1997) Improved and simplified in-gel sample application using rewelling of dry immobilized pH gradients. Electrophoresis 18:324–327

    Article  Google Scholar 

  13. Hou Yong, Xia Qingyou, Zhao Ping, Zou Yong, Liu Hongli, Guan Jian, Gong Jing, Xiang Zhonghuai (1997) Studies on middle and posterior silk glands of silkworm (Bombyx mori) using two-dimensional electrophoresis and mass spectrometry. Insect Biochem Mol Biol 37:486–496

    Article  Google Scholar 

  14. Jin Y-X, Chen Y-Y, Xu M-K, Jiang Y-H (2004) Studies on middle silkgland proteins of cocoon colour sex-limited silkworm (Bombyx mori L.) using two-dimensional polyacrylamide gel electrophoresis. J Biosci 29:45–49

    Article  Google Scholar 

  15. Nagaraju J (2000) Recent advances in molecular genetics of the silk moth, Bombyx mori. Curr Sci 78:151–161

    Google Scholar 

  16. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  Google Scholar 

  17. Koenig T, Menze BH, Kirchner M et al (2008) Robust prediction of the MASCOT score for an improved quality assessment in mass spectrometric proteomics. J Proteome Res 7(9):3708–3717

    Article  Google Scholar 

  18. Franceschini A, Szklarczyk D, Frankild S, Kuhn M, Simonovic M, Roth A, Lin J, Minguez P, Bork P, von Mering C, Jensen LJ (2013) STRING v9.1: protein–protein interaction networks, with increased coverage and integration. Nucleic Acids Res 41(Database issue):808–815

    Article  Google Scholar 

  19. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  Google Scholar 

Download references

Acknowledgments

AM is highly thankful to University Grants Commission New Delhi for a research fellowship. AM, AV, AB and JB performed the experiments. AM, PKY and DKG analyzed the data. DKG conceived the project.

Author information

Authors and Affiliations

  1. Department of Biotechnology, MNNIT Allahabad, Allahabad, 211004, India

    Ashutosh Mani

  2. Applied Molecular Biology Lab, SLS, Jawaharlal Nehru University, New Delhi, 110067, India

    Akhil Varshney, Ashima Bhaskar, Jyoti Bala & Pramod K. Yadava

  3. Center of Bioinformatics, University of Allahabad, Allahabad, 211002, India

    Ashutosh Mani & Dwijendra K. Gupta

Authors
  1. Ashutosh Mani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akhil Varshney
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ashima Bhaskar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jyoti Bala
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pramod K. Yadava
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dwijendra K. Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ashutosh Mani.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mani, A., Varshney, A., Bhaskar, A. et al. Differential Expression of Middle Silk Gland Proteins Caused by Cold Stress in Philosamia ricini . Natl. Acad. Sci. Lett. 39, 269–272 (2016). https://doi.org/10.1007/s40009-016-0479-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40009-016-0479-4

Keywords

Search

Navigation