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Development and Validation of HPTLC Method for the Estimation of Formaldehyde in Milk

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Abstract

The present study reports a rapid, routine and cost effective method using high performance thin layer chromatography (HPTLC) for the estimation of formaldehyde in milk samples. The standardization of the method was based on simulation parameters of chemical composition of mobile phase, nature of stationary phase and saturation time. Optimized stationary phase was aluminium baked HPTLC plates pre-coated with silica gel 60 F254s, mobile phase: chloroform: dichloromethane: diethyl ether (4:5:0.8, v/v/v), saturation time: 5 min. The proposed method was validated by testing its specificity, linearity, accuracy, precision, limit of detection and limit of quantification.

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Acknowledgments

The authors are grateful to Prof. P. K. Kalra, Director, Prof. Sahab Das, Head, Department of Chemistry, Dayalbagh Educational Institute, Dayalbagh, Agra. The authors are also thankful to University Grants Commission, New Delhi, for rendering financial assistance.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra, 282110, India

    Roopa Rani & M. M. Srivastava

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  1. Roopa Rani
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  2. M. M. Srivastava
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Correspondence to M. M. Srivastava.

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Rani, R., Srivastava, M.M. Development and Validation of HPTLC Method for the Estimation of Formaldehyde in Milk. Natl. Acad. Sci. Lett. 39, 21–24 (2016). https://doi.org/10.1007/s40009-015-0414-0

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  • DOI: https://doi.org/10.1007/s40009-015-0414-0

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