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Isolation of Ammonia Oxidizing Bacteria (AOB) from Fish Processing Effluents


In the present study, autotrophic ammonia oxidizing bacteria (AOB) were isolated and characterized from a fish processing effluent treatment plant. Sequencing of 16S rRNA gene showed AOB affiliation to Nitrosomonas marina, Nitrosomonas nitrosa and Nitrosospira lineages of Betaproteobacteria. The isolates were further confirmed with using ammonia mono-oxygenase subunit-A gene (amoA) specific fluorescence in situ hybridization assay. The reduction of ammonia by these isolates was comparable with the standard AOB pure culture Nitrosomonas europaea ATCC 19718. Among all the cultures, AOB-21 was found to be having highest potential for ammonia removal. The presence of AOB in fish processing effluent treatment plants suggests the usefulness of these isolates in sustainable removal of ammonia from fish processing waste effluent.

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Authors gratefully acknowledge the funding support to the research project under the ICAR- NAIP- National Fund for Basic, Strategic & Frontier Application Research in Agriculture. Grateful thanks to Prof. Daniel J. Arp, Oregon State University, USA for providing the Nitrosomonas europea ATCC 19718 culture.

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Correspondence to Indrani Karunasagar.

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Reddy, A.D., Subrahmanyam, G., NaveenKumar, S. et al. Isolation of Ammonia Oxidizing Bacteria (AOB) from Fish Processing Effluents. Natl. Acad. Sci. Lett. 38, 393–397 (2015).

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  • Ammonia oxidizing bacteria
  • Ammonia oxidation
  • Fish processing effluents
  • Fluorescence in situ hybridization
  • amoA gene
  • 16S rRNA gene