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Bioactive Potential of Marine Derived Strain Streptomyces brasiliensis VITJS9 Isolated from South East Coast of Tamil Nadu, India

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Abstract

The aim of the present investigation was to screen the bioactivity of Streptomyces sp. VITJS9 strain isolated from Kanyakumari, South East coast of India. The morphological, physiological, biochemical and phylogenetic properties of the strain was confirmed by conventional methods. The ethyl acetate crude extract tested for antibacterial activity showed better zone of inhibition at 30 mg/ml concentration against bacterial pathogens. In DPPH scavenging assay the IC50 value of the crude extract was found to be 150 µg/ml and showed cytotoxic effect with IC50 of 125 µg/ml on HeLa cells. The GC–MS analysis revealed the presence of three major chemical constituents namely N-Hexadecanoic acid, Octadecanoic acid and Dimethylsulfoxonium formylmethylide.

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References

  1. Skulberg OM (2000) Microalgae as a source of bioactive molecules–experience from cyanophyte research. J Appl Phycol 12:341–348

    Article  Google Scholar 

  2. Singh V, Praveen V, Khan F, Kant C, Tripathi M (2009) Phylogenetics of an antibiotic producing Streptomyces strain isolated from soil. Bioinfo 4(2):53–58

    Article  Google Scholar 

  3. Raja M, Ravikumar S, Gnanadesigan M, Vijayaumar V (2010) In vitro antibacterial activity of diterpene and benzoxazole derivatives from Excoecaria agallocha L. Int J Biol Chem Sci 4(3):692–701

  4. Williams ST, Cross T (1971) Isolation, purification, cultivation and preservation of actinomycetes. Methods Microbiol 4:295–334

    Article  Google Scholar 

  5. Nonomura H (1974) Key for classification and identification of 458 species of the Streptomycetes included in ISP. J Ferment Technol 52:78–92

    Google Scholar 

  6. Alexander M (1977) Introduction to soil microbiology, 2nd edn. Wiley, New York, p 207

    Google Scholar 

  7. Brock TD, Madigan MT, Martiko JM, Parker J (1994) Biology of microorganisms, 7th edn. Hall, International Inc, Englewood Cliffs

    Google Scholar 

  8. Prescott SC, Dunn GG (1959) Industrial microbiology, 3rd edn. McGraw-Hill, New York, pp 762–835

    Google Scholar 

  9. Remya M, Vijayakumar R (2007) Isolation and characterization of marine antagonistic actinomycetes from west coast of India. Med Biol 5:13–19

    Google Scholar 

  10. Pandey B, Ghimire P, Agrawal VP (2004) Studies on the antimicrobial activity of actinomycetes isolated from Khumbu region of Nepal. Ph.D dissertation, Tribhuvan University, Kathmandu

  11. Osawa T, Yoshida A, Kawakishi S, Yamashita K, Ochi H (1995) Protective role of dietary antioxidants in oxidative stress. In: Cutler RG, Packer L, Bertram J, Mori A (eds) Oxidative stress and aging. Birkhauser Verlag, Basel, pp 367–377

    Chapter  Google Scholar 

  12. Freshney RI, Hart E, Russell JM (1982) Isolation and purification of cell cultures from human tumours. In: Reid E, Cook GMW, Moore DJ (eds) Cancer cell organelles; methodological surveys (B): biochemistry, vol 2. Horwood, Chicester, pp 97–110

    Google Scholar 

  13. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

  14. Rainey FA, Ward-Rainey N, Kroppenstedt RM, Tackebrandt E (1996) The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092

    Article  Google Scholar 

  15. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  Google Scholar 

  16. Benson DA, Boguski MS, Lipman DJ, Ostell J, Oullette BFF, Rapp BA, Wheeler DL (1999) GenBank. Nucleic Acids Res 27:12–17

    Article  Google Scholar 

  17. Jemimah Naine S, Nasimunislam N, Vaishnavi B, Mohanasrinivasan V, Subathra Devi C (2012) Isolation of soil actinomycetes inhabiting amirithi forest for the potential source of bioactive compounds. Asian J Pharm Clin Res 5(4):189–192

    Google Scholar 

  18. Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS (2009) Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. Mar Drugs 7:24–44

  19. Dhanasekaran D, Selvamani S, Panneerselvam A, Thajuddin N (2009) Isolation and characterization of actinomycetes in Vellar Estuary, Annagkoil. Tamil Nadu African Afr J Biotechnol 8(17):4159–4162

    Google Scholar 

  20. Michael JM, Lavin MF, Watters DJ (1997) Resistance to radiation-induced apoptosis in Burkitt’s lymphoma cells is associated with defective ceramide signaling. Cancer Res 57(16):3600–3605

    Google Scholar 

  21. Lehmann S, Bengtzen S, Paul A, Christensson B, Paul C (2001) Effects of arsenic trioxide (As2O3) on leukemic cells from patients with non-M3 acute myelogenous leukemia: studies of cytotoxicity, apoptosis and the pattern of resistance. European Eur J Haematol 66(6):357–364

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Acknowledgment

The authors wish to thank VIT University for providing necessary facilities.

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Authors and Affiliations

  1. Industrial Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India

    S. Jemimah Naine, C. Subathra Devi, V. Mohanasrinivasan & B. Vaishnavi

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  1. S. Jemimah Naine
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  2. C. Subathra Devi
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  3. V. Mohanasrinivasan
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  4. B. Vaishnavi
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Correspondence to C. Subathra Devi.

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Jemimah Naine, S., Subathra Devi, C., Mohanasrinivasan, V. et al. Bioactive Potential of Marine Derived Strain Streptomyces brasiliensis VITJS9 Isolated from South East Coast of Tamil Nadu, India. Natl. Acad. Sci. Lett. 38, 221–224 (2015). https://doi.org/10.1007/s40009-014-0334-4

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  • DOI: https://doi.org/10.1007/s40009-014-0334-4

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