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Plants Genetically Engineered by use of Tissue Culture Based Transgenosis and Regeneration Procedures Require Genetic Background Purification by Backcross Breeding Before Their Application

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Abstract

Populations of transgenics produced by tissue culture based transgenosis and regeneration procedures are likely to be genetically heterogeneous because of unwanted presence of genetic and epigenetic changes. Before they are used in the definition of function specified by transgene or tested for biosafety and performance in the farmer’s fields, they should be in the first instance purified to genetic and epigenetic stability of the genetic background by backcross breeding.

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References

  1. Oksman-Caldentey K, Barz W (2002) Plant biotechnology and transgenic plants. Marcel Dekker, New York

    Book  Google Scholar 

  2. Lorz H, Wenzel G (2005) Molecular marker systems in plant breeding and crop improvement. Springer, New York

    Book  Google Scholar 

  3. Sleper DA, Poehlman JM (2006) Breeding fields crops. Amer, Iowa

    Google Scholar 

  4. Kouzarides T (2007) Chromatin modifications and their function. Cell 128:693–705

    Article  Google Scholar 

  5. Zhang X (2008) The epigenetic landscape of plants. Science 320:489–492

    Article  Google Scholar 

  6. Kumar S, Kumari R, Sharma V, Sharma V (2013) Roles, and establishment, maintenance and erasing of the epigenetic cytosine methylation marks in plants. J Genet 92:629–666

    Article  Google Scholar 

  7. Law JA, Jacobsen SE (2010) Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat Rev Genet 11:204–220

    Article  Google Scholar 

  8. Zhu JK (2009) Active DNA demethylation mediated by DNA glycosylases. Annu Rev Genet 43:143–166

    Article  Google Scholar 

  9. Johannes F, Porcher E, Teixeira FK, Saliba-Colombani V, Simon M et al (2009) Assessing the impact of transgenerational epigenetic variation on complex traits. PLoS Genet 5:e1000530

    Article  Google Scholar 

  10. Lisch D (2009) Epigenetic regulation of transposable elements in plants. Ann Rev Plant Biol 60:43–66

    Article  Google Scholar 

  11. Luna E, Bruce TJ, Roberts MR, Flors V, Ton J (2012) Next-generation systemic acquired resistance. Plant Physiol 158:844–853

    Article  Google Scholar 

  12. Slaughter A, Daniel X, Flors V, Luna E, Hohn B, Mauch-Mani B (2012) Descendants of primed Arabidopsis plants exhibit resistance to biotic stress. Plant Physiol 158:835–843

    Article  Google Scholar 

  13. Pfeifer GP (2006) Mutagenesis at methylated CpG sequences. CTMI 301:259–281

    Google Scholar 

  14. Walsch CP, Xu GL (2006) Cytosine methylation and DNA repair. CTMI 301:283–315

    Google Scholar 

  15. Pavet V, Quintero C, Cecchini NM, Rosa AL, Alvarez ME (2006) Arabidopsis displays centromeric DNA hypomethylation and cytological alterations of heterochromatin upon attack by Pseudomonas syringae. Mol Plant Microbe Interact 19:577–587

    Article  Google Scholar 

  16. Choi CS, Sano H (2007) Abiotic-stress induces demethylation and transcriptional activation of a gene encoding a glycerophosphodiesterase-like protein in tobacco plants. Mol Genet Genomics 277:589–600

    Article  Google Scholar 

  17. Verhoeven KJF, Jansen JJ, van Dijk PJ, Biere A (2010) Stress-induced DNA methylation changes and their heritibility in asexual dandelions. New Phytol 185:1108–1118

    Article  Google Scholar 

  18. Bilichak A, Ilnystkyy Y, Hollunder J, Kovalchuk I (2012) The progeny of Arabidopsis thaliana plants exposed to salt exhibit changes in DNA methylation, histone modification and gene expression. PLoS One 7:e30515

    Article  Google Scholar 

  19. Rasmann S, De Vos M, Casteel CL, Tian D, Halitschke R, Sun JY et al (2012) Herbivory in the previous generation primes plants for enhanced insect resistance. Plant Physiol 158:854–863

    Article  Google Scholar 

  20. Miguel C, Marum L (2011) An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond. J Exp Bot 62:3713–3725

    Article  Google Scholar 

  21. Smulders MJM, de Klerk GJ (2011) Epigenetics in plant tissue culture. Plant Growth Regul 63:137–146

    Article  Google Scholar 

  22. Schellenbaum P, Mohler V, Wenzel G, Walter B (2008) Variation in DNA methylation patterns of grapevine somaclones (Vitis Vinifera L.). BMC Plant Biol 8:78

    Article  Google Scholar 

  23. Sharma S, Bryan G, Winfield M, Millam S (2007) Stability of potato (Solanum tuberosum L.) plants regenerated via somatic embryos, axillary bud proliferated shoots, microtubers and true potato seeds: a comparative phenotypics cytogenetic and molecular assessment. Planta 226:1449–1458

    Article  Google Scholar 

  24. Paredo EL, Revilla MA, Arroyo-García R (2006) Assessment of genetic and epigenetic variation in hop plants regenerated from sequential subcultures of organogenic calli. J Plant Physiol 163:1071–1079

    Article  Google Scholar 

  25. Jaligot E, Beule T, Baurens FC, Billotte N, Rival A (2004) Search for methylation-sensitive amplification polymorphism associated with the ‘mantled’ variant phenotype in oil palm (Elaeis guineensis Jacq.). Genome 47:224–228

    Article  Google Scholar 

  26. Zhang M, Xu C, Yan H, Zhao N, Von Wettstein D, Liu B (2009) Limited tissue culture-induced mutations and linked epigenetic modifications in F1 hybrids of sorghum pure lines and accompanied by increased transcription of DNA methyltransferases and 5-methylcytosine glycosylases. Annu Rev Genet 43:143–166

    Article  Google Scholar 

  27. Yu X, Li X, Zhao X, Jiang L, Miao G, Pang J, Qi X, Liu B (2011) Tissue culture-induced genomic alteration in maize (Zea mays) inbred lines and F1 hybrids. Ann Appl Biol 158:237–247

    Article  Google Scholar 

  28. Wang X, Wu R, Lin X, Bai Y, Song C, Yu X, Xu C, Zhao N, Dong Y, Liu B (2013) Tissue culture-induced genetic and epigenetic alterations in rice pure-lines, F1 hybrids and polyploids. BMC Plant Biol 13:77

    Article  Google Scholar 

  29. Stroud H, Ding B, Simon SA, Feng S, Bellizzi M, Pellegrini M, Wang G-L, Meyers BC, Jacobsen SE (2013) Plants regenerated from tissue culture contain stable epigenome changes in rice. eLIFE 2:e00354

    Article  Google Scholar 

  30. Yang C, Chen H, Tang W, Lin Y-J (2012) Effect of successive backcrossing on eliminating somaclonal variation caused by Agrobacterium-mediated transformation in rice. Acta Agron Sin 38:814–819

    Article  Google Scholar 

  31. Bregitzer P, Dahleen LS, Neate S, Schwarz P, Manoharan M (2008) A single backcross effectively eliminates agronomic and quality alterations caused by somaclonal variation in transgenic barley. Crop Sci 48:471–479

    Article  Google Scholar 

  32. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743

    Article  Google Scholar 

  33. Zhou B, Qu S, Liu G, Dolan M, Sakai H, Lu G, Bellizzi M, Wang GL (2006) The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea. Mol Plant Microbe Interact 19:1216–1228

    Article  Google Scholar 

  34. Zhang Q, Folta KM, Davis TM (2014) Somatic embryogenesis, tetraploidy and variant leaf morphology in transgenic diploid strawberry (Fragaria vesca subspecies vesca ‘Hawaii 4’). BMC Plant Biol 14:23

    Article  Google Scholar 

  35. Becker C, Hagmann J, Muller J, Koenig D, Stegle O, Borgwardt K, Weigel D (2011) Spontaneous epigenetic variation in the Arabidopsis thaliana methylome. Nature 480:245–249

    Article  Google Scholar 

  36. Otagaki S, Kasai M, Masuta C, Kanazawa A (2013) Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1 ortholog using a virus vector in Nicotiana benthamiana. Front Genet 4: Article44, 1-10

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Authors and Affiliations

  1. Education and Development (SKAIRED), SKA Institution for Research, 4/11 Sarv Priya Vihar, New Delhi, 110016, India

    Sushil Kumar, Renu Kumari & Vishakha Sharma

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  1. Sushil Kumar
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  2. Renu Kumari
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  3. Vishakha Sharma
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Correspondence to Sushil Kumar.

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Kumar, S., Kumari, R. & Sharma, V. Plants Genetically Engineered by use of Tissue Culture Based Transgenosis and Regeneration Procedures Require Genetic Background Purification by Backcross Breeding Before Their Application. Natl. Acad. Sci. Lett. 37, 573–577 (2014). https://doi.org/10.1007/s40009-014-0282-z

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