Skip to main content

Assessment of Genetic Diversity in Valeriana jatamansi Jones. Germplasm Using RAPD Markers

Abstract

Randomly amplified polymorphic DNA markers were used to evaluate the genetic diversity in a representative population of Valeriana jatamansi Jones., an important medicinal herb from different phytogeographical regions of northern Himalayas. A total of 18 accessions were screened using 79 random oligonucleotide decamer primers. Of the 22 selected screened primers, a total of 106 bands were obtained, representing 89.62 % of the total amplification products. The polymorphism was scored and used in band sharing analysis to identify genetic relationships. Similarity indices ranged from 0.53 to 0.85, indicating a moderate to high genetic variability among the genotypes. 15 genotype specific markers in 12 genotypes of V. jatamansi were detected. The patterns distinguished between the accessions and their analysis established an approach for classification within V. jatamansi germplasm on the basis of these RAPD markers. The accessions from the sites Dharmkot (11) and Chail-I (1) had least similarity (53 %) and therefore were the most divergent.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  1. Raina R, Srivastava SK (1992) Floral polymorphism in Valeriana jatamansi Jones. Indian J Plant Gen Res 5:93–94

    Google Scholar 

  2. Becker H, Chavadej S (1988) Valepotriates: production by plant cell cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, medicinal and aromatic plants, vol 4. Springer, Berlin, pp 294–309

    Chapter  Google Scholar 

  3. Joshi R, Nailwal TK, Tewari M, Shukla A (2009) Exploring biotechnology for conserving himalayan biodiversity. Researcher 1:36–45

    Google Scholar 

  4. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  Google Scholar 

  5. Rafalski JA, Tingey SV (1993) Genetic diagnosis in plant breeding: RAPDs, microsatellites and machines. Trends Genet 9:275–280

    Article  Google Scholar 

  6. Cheng KT, Chang HC, Huang H, Lin CT (2000) RAPD analysis of Lycium barbarum medicine in Taiwan market. Bot Bull Acad Sin 41:11–14

    Google Scholar 

  7. Sharma KD, Singh BM, Sharma TR, Katoch M, Guleria S (2000) Molecular analysis of variability in Podophyllum hexandrum Royle—an endangered medicinal herb of northwest Himalaya. Plant Gen Res Newsl 124:57–61

    Google Scholar 

  8. Alam MA, Gulati P, Gulati AK, Mishra GP, Naik PK (2009) Assessment of genetic diversity among Podophyllum hexandrum genotypes of the North-western Himalayan region for podophyllotoxin production. Indian J Biotechnol 8:391–399

    Google Scholar 

  9. Betal S, Chowdhury PR, Kundu S, Raychaudhary SS (2004) Estimation of genetic variability of Vigna radiata cultivars by RAPD analysis. Bio Plant 48:205–209

    Article  Google Scholar 

  10. Cordeiro AI, Sanchez-Sevilla JF, Alvarez-Tinaut MC, Gomez-Jimenez MC (2008) Genetic diversity assessment in Portugal accessions of Olea europea by RAPD markers. Biol Plant 52:642–647

    Article  Google Scholar 

  11. Pandey S, Kumar S, Mishra U, Rai A, Singh M, Rai M (2008) Genetic diversity in Indian ash gourd (Benincasa hispida) accessions as revealed by quantitative traits and RAPD markers. Sci Hortic 118:80–86

    Article  Google Scholar 

  12. Choudhury PR, Singh IP, George B, Verma AK, Singh NP (2008) Assessment of genetic diversity of pigeonpea cultivars using RAPD analysis. Bio Plant 52:648–653

    Article  Google Scholar 

  13. Jordan-Pla A, Estrelles E, Boscaiu M, Soriano P, Vicente O, Mateu-Andres I (2009) Genetic variability in the endemic Leucojum valentinum. Biol Plant 53:317–320

    Article  Google Scholar 

  14. Padmesh P, Reji JV, Paul B, Mukunthakumar S, Praveen G, Seeni S (2009) Analysis of genetic variability in two diploid Musa cultivars using RAPD. Biol Plant 53:711–714

    Article  Google Scholar 

  15. Ikbal BKS, Dhillon RS (2010) Evaluation of genetic diversity in Jatropha curcas L. using RAPD markers. Indian J Biotechnol 9:50–57

    Google Scholar 

  16. Singh SK, Harsh LN, Pancholy A, Pathak R, Raturi A (2011) Molecular assessment of inter-specific genetic diversity in selected species of Prosopis revealed by RAPD. Indian J Agric Sci 81:67–171

    Google Scholar 

  17. Jindal SK, Tak A, Singh SK, Pancholy A, Pathak R, Raturi A (2011) Molecular assessment of genetic diversity in Acacia senegal. Indian J Agric Sci 81:695–699

    Google Scholar 

  18. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  19. Rohlf FJ (1997) NTSYS pc: numerical taxonomy and multivariate analysis system version 2.02 h. Exeter software, New York

  20. Prakash V (1999) Indian Valerianaceae: a monograph on a medicinally important family. Scientific Publishers, Jodhpur

    Google Scholar 

  21. Saikia Q, Khamjir PS, Shasany AK, Darokar MP, Kukreja AK, Kumar S (2000) Assessment of diversity among Taxus wallichiana accessions from north east India using RAPD analysis. Plant Gen Res Newslett 121:27–31

    Google Scholar 

  22. Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In: Brown ADH, Clegg MT, Kahler AL, Weir BS (eds) Plant population genetics, breeding and genetic resources. Sinauer, Sunderland, pp 304–319

    Google Scholar 

  23. Jain N, Shasany AK, Sundaresan V, Rajkumar S, Darokar MP, Bagchi GD, Gupta AK, Kumar S, Khanuja SPS (2003) Molecular diversity in Phyllanthus amarus assessed through RAPD analysis. Curr Sci 85:1454–1458

    Google Scholar 

  24. Bantawa P, Das A, Ghosh PD, Mondal TK (2011) Detection of natural genetic diversity of landraces by RAPDs: an endangered woody oil bearing plant of Indo-China Himalayas. Indian J Biotechnol 10:294–300

    Google Scholar 

  25. Bharmauria V, Narang N, Verma V, Sharma S (2009) Genetic variation and polymorphism in the Himalayan nettle plant Urtica dioica based on RAPD marker. J Med Plants Res 3:166–170

    Google Scholar 

  26. Shaw RK, Acharya L, Mukherjee AK (2009) Assessment of genetic diversity in a highly valuable medicinal plant Catharanthus roseus using molecular markers. Crop Breed Appl Biotechnol 9:52–59

    Google Scholar 

Download references

Acknowledgments

The authors are thankful to the Professor and Head, Dept. of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, INDIA and Dr. T. Mohapatra, Principal Scientist (Biotechnology), NRCPB, New Delhi for providing all the necessary facilities to carry out the research work.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ajay Kumar Thakur.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kumar, A., Shirkot, P., Thakur, A.K. et al. Assessment of Genetic Diversity in Valeriana jatamansi Jones. Germplasm Using RAPD Markers. Natl. Acad. Sci. Lett. 35, 221–226 (2012). https://doi.org/10.1007/s40009-012-0045-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40009-012-0045-7

Keywords

  • Valeriana jatamansi Jones.
  • RAPD markers
  • Genetic diversity
  • Molecular characterization