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National Academy Science Letters

, Volume 35, Issue 3, pp 221–226 | Cite as

Assessment of Genetic Diversity in Valeriana jatamansi Jones. Germplasm Using RAPD Markers

  • Amit Kumar
  • Poonam Shirkot
  • Ajay Kumar ThakurEmail author
  • R. Raina
Research Article

Abstract

Randomly amplified polymorphic DNA markers were used to evaluate the genetic diversity in a representative population of Valeriana jatamansi Jones., an important medicinal herb from different phytogeographical regions of northern Himalayas. A total of 18 accessions were screened using 79 random oligonucleotide decamer primers. Of the 22 selected screened primers, a total of 106 bands were obtained, representing 89.62 % of the total amplification products. The polymorphism was scored and used in band sharing analysis to identify genetic relationships. Similarity indices ranged from 0.53 to 0.85, indicating a moderate to high genetic variability among the genotypes. 15 genotype specific markers in 12 genotypes of V. jatamansi were detected. The patterns distinguished between the accessions and their analysis established an approach for classification within V. jatamansi germplasm on the basis of these RAPD markers. The accessions from the sites Dharmkot (11) and Chail-I (1) had least similarity (53 %) and therefore were the most divergent.

Keywords

Valeriana jatamansi Jones. RAPD markers Genetic diversity Molecular characterization 

Notes

Acknowledgments

The authors are thankful to the Professor and Head, Dept. of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, INDIA and Dr. T. Mohapatra, Principal Scientist (Biotechnology), NRCPB, New Delhi for providing all the necessary facilities to carry out the research work.

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Copyright information

© The National Academy of Sciences, India 2012

Authors and Affiliations

  • Amit Kumar
    • 1
  • Poonam Shirkot
    • 1
  • Ajay Kumar Thakur
    • 1
    Email author
  • R. Raina
    • 2
  1. 1.Department of BiotechnologyDr. Y.S. Parmar University of Horticulture and ForestryNauni, SolanIndia
  2. 2.Department of Forest ProductsDr. Y.S. Parmar University of Horticulture and ForestryNauni, SolanIndia

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