Abstract
Purpose
The pharmaceutical industry has been searching for drugs that aim at the prevention, the protection, and the recovery of the skin. Thus, the aim of this study was to evaluate the anti-inflammatory and anti-edematogenic activities of a topical emulsion containing Helianthus annuus oil (5%), glycerin (30%), and vitamin B3 (5%).
Methods
The aim was verified through chronic inflammatory responses and edema that were induced by irritant croton oil in the ears of mice, followed by histological and leukocyte infiltration. The study also affirmed the stability and the cytotoxicity of the emulsion.
Results
A stable, homogeneous, shiny, slightly-white, and gelatinous emulsion was obtained. The cytotoxicity evaluation allowed for the verification that only in high concentrations were unsatisfactory results presented. The treatment was effective in reversing the inflammatory parameters that were induced by croton oil, such as edema, neutrophil migration, and cell hyperproliferation.
Conclusion
The developed emulsion containing sunflower oil, glycerin, and vitamin B3 had parameters of stability and presented anti-inflammatory activities. The researchers’ findings suggest that the topical use of this formulation is a new and novel strategy for the treatment of inflammatory conditions of the skin.
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AHS and LMF conceived and designed the research. APC, EW, L de MF, AR, MFPF, and GJVA conducted the experiments. FTTA, INR, AHS, ESO, and IG analyzed the data. FTTA, LMF, INR, AHS, and IG wrote the manuscript. All of the authors have read and approved the manuscript for publication.
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The study was carried out in accordance with the Institutional Guidelines for the Care and Use of Animals and the Ethical Standards of the Lutheran University of Brazil (ULBRA, Canoas, Rio Grande do Sul, Brazil). The procedures were conducted after previous approbation from the Institutional Research Ethics Committee under the protocol 2014-30P.
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All applicable International, National, and/or Institutional Guidelines for the Care and Use of Animals were followed. All of the procedures that were performed in the studies involving the animals were conducted in accordance with the Ethical Standards of the Research Ethics Committee of the Lutheran University of Brazil (ULBRA, Canoas, Rio Grande do Sul, Brazil), and this University is where the studies were administered under protocol 2014-30P.
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Freitas, L.M., Antunes, F.T.T., Obach, E.S. et al. Anti-inflammatory effects of a topical emulsion containing Helianthus annuus oil, glycerin, and vitamin B3 in mice. J. Pharm. Investig. 51, 223–232 (2021). https://doi.org/10.1007/s40005-020-00508-6
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DOI: https://doi.org/10.1007/s40005-020-00508-6