Journal of Pharmaceutical Investigation

, Volume 46, Issue 3, pp 283–291 | Cite as

Pharmacokinetic evaluation of cefdinir-loaded floating alginate beads in rabbits using LC–MS/MS

  • Praveen R
  • Sandeep Kumar Singh
  • Priya Ranjan Prasad Verma


The present investigation aims to compare the pharmacokinetic parameters of Cefdinir in rabbits, from floating alginate (an anionic polysaccharide obtained from cell walls of brown algae) beads and conventional suspension, using a new LC–MS/MS method. Formulations equivalent to 20 mg/kg were administered orally to test and reference group and blood samples were collected at selected time intervals up to 24 h. Plasma concentrations of Cefdinir were determined using validated LC–MS/MS method and pharmacokinetic parameters were derived by non-compartment model. Statistically significant (p < 0.05) increase in Cmax, Tmax, AUC0–∞ and MRT was observed in case of floating alginate beads, whereas KE and t1/2 remained relatively constant. MRT and tmax increased significantly as a result of controlled drug release. Relative bioavailability was 337.45 % for the floating beads. Thus, alginate based floating formulation improve the bioavailability (3.37 fold) of Cefdinir compared to suspension. The absorption of Cefdinir from floating beads was found mainly from duodenum (73.0 %) and Jejunum 1 and 2 (13.0 %).


Alginate Bioavailability Gastroretentive 



All authors (Praveen R, S. K. Singh, P. R. P. Verma) declare that they have no conflict of interest. Praveen R, is thankful to UGC, New Delhi, India for financial assistance in the form of basic scientific research fellowship (Ref.: F.7-32/2007).


  1. FDA Guidance for Industry (2013) Bioanalytical Method Validation, US Department of Health and Human Services, Food and Drug Administration, Center for drug Evaluation and Research (CDER).
  2. Guay DRP (2000a) Cefdinir: an expanded-spectrum oral cephalosporin. Ann Pharmacother 34(12):1469–1477CrossRefPubMedGoogle Scholar
  3. Guay DRP (2000b) Pharmacodynamics and pharmacokinetics of cefdinir, an oral extended spectrum cephalosporin. Pediatr Infect Dis J 19(12):S141–S146CrossRefPubMedGoogle Scholar
  4. Hishida A, Ohishi K, Nagashima S, Kanamaru M, Obara M, Kitada A (1998) Pharmacokinetic study of an oral cephalosporin, cefdinir, in hemodialysis patients. Antimicrob Agents Chemother 42(7):1718–1721PubMedPubMedCentralGoogle Scholar
  5. Jana S, Gangopadhaya A, Bhowmik BB, Nayak AK, Mukherjee A (2015) Pharmacokinetic evaluation of testosterone-loaded nanocapsules in rats. Int J Biol Macromol 72:28–30CrossRefPubMedGoogle Scholar
  6. Jin H-E, Kim I-B, Kim C-K, Maeng H-J (2013) Determination of cefdinir levels in rat plasma and urine by high-performance liquid chromatography-tandem mass spectrometry: application to pharmacokinetics after oral and intravenous administration of cefdinir. Biomed Chromatogr 27:1423–1430CrossRefPubMedGoogle Scholar
  7. Joseph NJ, Lakshmi S, Jayakrishnan A (2002) A floating-type oral dosage form for piroxicam based on hollow polycarbonate microspheres: in vitro and in vivo evaluation in rabbits. J Control Release 79:71–79CrossRefPubMedGoogle Scholar
  8. Okamoto Y, Itoh K, Namiki Y, Matsushita J, Fujioka M, Yasuda T (1996) Method development for the determination of cefdinir and its related substances by high-performance liquid chromatography. J Pharm Biomed Anal 14:739–748CrossRefPubMedGoogle Scholar
  9. Praveen R, Singh SK, Verma PRP, George JK (2014) Sustained delivery of cefdinir to upper gastrointestinal tract using calcium alginate beads: a formulation by design. J Pharm Investig 44(6):455–463CrossRefGoogle Scholar
  10. Praveen R, Singh SK, Verma PRP, George JK (2015) Cross linked alginate gel beads as floating drug delivery system for cefdinir: optimization using Box-Behnken design. J Pharm Investig 45(2):187–199CrossRefGoogle Scholar
  11. Tsuji A, Tamai I, Nakanishi M, Terasaki T, Hamano S (1993) Intestinal brush-border transport of the oral cephalosporin antibiotic, cefdinir, mediated by dipeptide and monocarboxylic acid transport systems in rabbits. J Pharm Pharmacol 45:996–998CrossRefPubMedGoogle Scholar

Copyright information

© The Korean Society of Pharmaceutical Sciences and Technology 2016

Authors and Affiliations

  • Praveen R
    • 1
  • Sandeep Kumar Singh
    • 1
  • Priya Ranjan Prasad Verma
    • 1
  1. 1.Department of Pharmaceutical Sciences and TechnologyBirla Institute of Technology, MesraRanchiIndia

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