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Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in sodium alginate beads with or without hyaluronic acid

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Abstract

Human adipose-derived mesenchymal stem cells (AD-MSCs) have attracted much interest as an alternative to autologous chondrocytes and bone marrow-derived mesenchymal stem cells for cell-based therapy to repair cartilage defects. Sodium alginate (SA) beads have been widely known as a conventional stem cell delivery system for cartilage repair. Hyaluronic acid (HA) has been known to induce cell proliferation and chondrogenic differentiation. Herein, we prepared AD-MSCs-encapsulating SA beads with HA (SA–HA beads) and without HA (SA beads). Then, the morphology, proliferation, and chondrogenic differentiation of AD-MSCs cultured in SA–HA beads or SA beads with a conventional chondrogenic media were evaluated. There was no discernible difference in the morphology of AD-MSCs between SA–HA and SA beads. However, the proliferation (MTT optical density and DNA contents) and chondrogenic differentiation (s-GAG contents and type II collagen staining) of AD-MSCs were significantly enhanced in SA–HA beads as compared to SA beads. The present results suggest that HA can be added to SA beads-based cell delivery systems of AD-MSCs in order to improve their chondrogenesis-inducing capacity for repair of cartilage defects.

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Abbreviations

SA:

Sodium alginate

HA:

Hyaluronic acid

MSCs:

Mesenchymal stem cells

AD-MSCs:

Adipose-derived mesenchymal stem cells

BM-MSCs:

Bone marrow-derived mesenchymal stem cells

DMEM-HG:

Dulbecco’s modified eagle’s medium–high glucose

TGF-β1:

Transforming growth factor-β1

ITS:

Insulin–transferrin–selenium

CM:

Chondrogenic medium (serum-free DMEM-HG supplemented with 10 ng/mL of TGF-β1, 50 nM ascorbate, 100 nM dexamethasone, and 5 μg/mL of ITS)

EM:

Expansion medium (DMEM-HG supplemented with 10 % fetal bovine serum)

SA–HA beads:

HA-containing SA beads

SA beads:

HA-free SA beads

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Acknowledgments

This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (No. 2011-0030635).

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Authors and Affiliations

  1. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 151-742, Republic of Korea

    Dong-Hwan Kim & Dae-Duk Kim

  2. College of Pharmacy, Mokpo National University, Jeonnam, 534-729, Republic of Korea

    In-Soo Yoon

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  1. Dong-Hwan Kim
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Correspondence to In-Soo Yoon.

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Kim, DH., Kim, DD. & Yoon, IS. Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in sodium alginate beads with or without hyaluronic acid. Journal of Pharmaceutical Investigation 43, 145–151 (2013). https://doi.org/10.1007/s40005-013-0059-2

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