Skip to main content
SpringerLink
Log in

Heterosis Breeding Could Improve Root and Stem Anatomical Traits Conferring Bacterial Wilt Disease Tolerance in Tomato Cultivars

  • Full-Length Research Article
  • Published:
Agricultural Research Aims and scope Submit manuscript

Abstract

Twelve F1 hybrids developed through line × tester design along with seven parents were evaluated for fruit yield, quality, incidence of bacterial wilt disease and anatomical features of root and stem to study the manifestation of heterobeltiosis, and to understand the implication of anatomical characters towards tolerance against bacterial wilt disease. Two hybrids (Utkal Deepti × CLN-2764A and Utkal Kumari × CLN-2777G) registering substantial tolerance against bacterial wilt exhibited marked heterobeltiosis for fruit yield per plant and other component traits. Hypocotyls of the highly tolerant hybrid did not show any bacterial colonization in xylem tissues under light microscope even at 8 days of inoculation with native bacterial isolate. The potted seedlings of tolerant hybrid showed mean disease index of 1.16 even at 20 days of inoculation with bacterial isolate which indicated promise for its utilization in bacterial wilt prone areas. Establishment of significant negative correlations between the incidence of bacterial wilt disease and the ratio of large vessels/small vessels in stem, diameter of large vessels in stem, and diameter of large vessels in roots suggested that these anatomical characters might be considered as important indices for selection of hybrid tolerant to bacterial wilt disease. Significant heterobeltiosis was also manifested for three anatomical traits. This study justified the scope of utilizing the anatomical features of stem and root in breeding tomato cultivars tolerant to bacterial wilt disease. Overwhelming response of non-additive genetic control for these anatomical traits also exposed the evidence of heterosis breeding for their improvement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Abdullah H, Rahman MA (1998) Multiplication of Ralstonia solanacearum in Capsicum annnum. In: Prior P, Allen C, Elphinstone J (eds) Bacterial wilt disease: molecular and ecological aspects. New York

    Google Scholar 

  2. Acosta JE, Gilbert IC, Quinon VL (1964) Heritability of bacterial wilt resistance in tomato. Proc Amer Soc Hort Sci 84:55

    Google Scholar 

  3. Álvarez B, Biosca EG, López MM (2010) On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen. In: Méndez-Vilas A (ed) Microbial Biotechnology. Formatex, Bada-joz

    Google Scholar 

  4. Baker RJ (1978) Issues in diallel analysis. Crop Sci 18:533–536

    Article  Google Scholar 

  5. Beckman CH (1987) The nature of wilt diseases of plants. APS press, St. Paul, MN

    Google Scholar 

  6. Beckman CH, Brun WA, Buddenhagen IW (1962) Water relations in banana plants infected with Pseudomonas solanacearum. Phytopath 52:1144–1148

    CAS  Google Scholar 

  7. Bhutia ND, Seth T, Shende VD, Dutta S, Chattopadhyay A (2015) Estimation of heterosis, dominance effect and genetic control of fresh fruit yield, quality and leaf curl disease severity traits of chilli pepper (Capsicum annuum L.). Sci Hort 182:47–55

    Article  Google Scholar 

  8. Buddenhagen IW, Kelman A (1964) Biological and physiological aspects of bacterial wilt caused by Pseudomonas solanacearum. Ann Rev Phytopath 2:203–230

    Article  Google Scholar 

  9. Caldwell D, Caldwell D, Kim B-S, Anjali S, Iyer-Pascuzzi (2017) Ralstonia solanacearum differentially colonizes roots of resistant and susceptible tomato plants. Phytopath 107:528–536

    Article  CAS  Google Scholar 

  10. Chattopadhyay A, Dutta S, Bhattacharya I, Karmakar K, Hazra P (2007) Technology for vegetable crop production. In: All India coordinated research project on vegetable crops. Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Nadia, West Bengal, p 226.

    Google Scholar 

  11. Chattopadhyay A, Dutta S, Dutta P, Hazra P (2011) Studies on heterobeltiosis, combining ability and gene action in tomato (Solanum lycopersicum). Int J Pl Breed 5(2):88–93

    Google Scholar 

  12. Chattopadhyay A, Seth T, Dutta S, Ghosh PP, Chattopadhyay SB, Majumdar D, Hazra P (2012) Breeding eggplant for higher productivity and bacterial wilt tolerance. Int J Veg Sci 18(4):376–392

    Article  Google Scholar 

  13. Dahal D, Heintz D, Van Dorsselaer A, Braun HP, Wydra K (2009) Pathogenesis and stress related, as well as metabolic proteins are regulated in tomato stems infected with Ralstonia solanacearum. Pl Physiol Biochem 47:838–846

    Article  CAS  Google Scholar 

  14. Danesh D, Aarons S, McGill GE, Young ND (1994) Genetic dissection of oligogenic resistance to bacterial wilt in tomato. Mol Plant Microbe Interact 7:464–471

    Article  CAS  PubMed  Google Scholar 

  15. Dutta P, Rahman B (2012) Varietal screening of tomato against bacterial wilt disease under subtropical humid climate of Tripura. Int J Farm Sci 2(2):40–43

    Google Scholar 

  16. Fegan M, Prior P (2005) How complex is the Ralstonia solanacearum species complex? In: Allen C, Hayward AC (eds) American Phytopathological Society Press St Paul Bacterial wilt: the Disease and the Ralstonia solanacearum Species Complex. MN USA

    Google Scholar 

  17. Ghosh PP, Dutta S, Seth T, Chattopadhyay A (2015) Influence of stem and root vascular anatomy of eggplant germplasm on severity of vascular bacterial wilt disease. Indian Phytopath 68(4):363–367

    Google Scholar 

  18. Göhre V, Robatzek S (2008) Breaking the barriers: microbial effector molecules subvert plant immunity. Ann Rev Phytopath 46:189–215

    Article  CAS  Google Scholar 

  19. Gomez KA, Gomez AA (1984) Statistical Procedures for Agricultural Research, 2nd edn. John Wiley and Sons, New York USA

    Google Scholar 

  20. Griffing B (1956) Concept of general and specific combining ability in relation to diallel cross system. Aust J Biol Sci 9:463–493

    Article  Google Scholar 

  21. Grimault V, Gélie B, Lemattre M, Prior P, Schmit J (1994) Comparative histology of resistant and susceptible tomato cultivars infected by Pseudomonas solanacearum. Physiol Mol Plant Pathol 44:105–123

    Article  Google Scholar 

  22. Grimault V, Prior P, Anais G (1995) A monogenic dominant resistance of tomato to bacterial wilt in Hawaii 7996 is associated with plant colonization by Pseudomonas solanacearum. J Phytopath 143:349–352

    Article  Google Scholar 

  23. Hannan MM, Ahmed MB, Razvy MA, Karim R, Khatun M, Haydar A, Hossain M, Roy UK (2007) Heterosis and correlation of yield and yield components in tomato (Lycopersicon esculentum Mill). Amer Eur J Sci Res. 2(2):146–150

    Google Scholar 

  24. Hayes HK, Immer FR, Smith DC (1965) Methods of Plant Breeding, Biotech Books India, P. 552.

  25. Ishihara T, Mitsuhara I, Takahashi H, Nakaho K (2012) Transcriptome analysis of quantitative resistance-specific response upon Ralstonia solanacearum infection in tomato. PLoS ONE 7:e46763

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Kelman A (1954) The relationship of pathogenicity of Pseudomonas solanacearum to colony appearance in a tetrazolium medium. Phytopath 44:693–695

    Google Scholar 

  27. Gyu KS, On-Sook H, Na-Young Ro, Ho-Cheol Ko, Ju-Hee R, Sook SJ, Kyoung-Yul R, Sok-Young L, Jin BH (2016) Evaluation of resistance to Ralstonia solanacearum in tomato genetic resources at seedling stage. Plant Pathol J 32(1):58–64

    Article  Google Scholar 

  28. Kumar R, Srivastava K, Singh NP, Vasistha NK, Singh RK, Singh MK (2013) Combining ability analysis for yield and quality traits in tomato (Solanum lycopersicum L.). J Agric Sci 5(2):213–218

    Google Scholar 

  29. Lowe-Power TM, Devanshi K, Caitilyn A (2018) How Ralstonia solanacearum exploits and thrives in the flowing plant xylem environment. Trends Microbiol 26(11):929–942

    Article  CAS  PubMed  Google Scholar 

  30. Manna BK, Jash S, Das SN (2003) Field Screening of brinjal germplasm lines against bacterial wilt. Environ Ecol 21(3):730–732

    Google Scholar 

  31. Opena RT, Green SK, Talekar NS, Chen JT (1990) Genetic Improvement of Tomato Adaptability to the Tropics. In: Green SK, Griggs T, McLean BT (eds) Tomato and Pepper Production in the Tropics. Asian Vegetable Research and Development Center, Tainan, Taiwan

    Google Scholar 

  32. Pandiarana N, Chattopadhyay A, Seth T, Shende VD, Dutta S, Hazra P (2015) Heterobeltiosis, potence ratio and genetic control of processing quality and disease severity traits in tomato. NZ J Crop Hort Sci 43(4):282–293

    Article  CAS  Google Scholar 

  33. Papenfort K, Bassler BL (2016) Quorum sensing signal-response systems in Gram-negative bacteria. Nat Rev Microbiol 14:576–588

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Peter KV, Goth RW, Webb RE (1984) Indian hot pepper as new source of resistance to bacterial wilt, Phytophthora root rot and root knot nematode. HortScience 19:227–278

    Google Scholar 

  35. Prior P, Bart S, Leclercq S, Darrasse A, Anais G (1996) Resistance to bacterial wilt in tomato as discerned by spread of Pseudomonas (Burholderia) solanacearum in the stem tissues. Pl Path 45:720–726

    Article  Google Scholar 

  36. Prior P, Grimault V, Schmit J (1994) Resistance to bacterial wilt (Pseudomonas solanacearum) in tomato: Present status and prospects. In: Hayward AC, Hartman GL (eds) Bacterial Wilt: The Disease and its Causative Agent Pseudomonas solanacearum. CAB International Wallingford, England

    Google Scholar 

  37. Rahman MA, Abdullah H (1997) Susceptibility of capsicum species and cultivars to Ralstonia solanacearum; anatomical differences and bacterial multiplication in resistant and susceptible cultivars. Pertanika J Trop Agric Sci 20(1):1–11

    Google Scholar 

  38. Ranganna S (2001) Analysis and quality control for fruits and vegetable products, 2nd edn. Tata McGraw-Hill Publication, New Delhi, India, pp 110–112

    Google Scholar 

  39. Rashid MA, Singh DP (2000) A manual on vegetable seed production in Bangladesh. AVRDC-USAID-Bangladesh project, Horticultural Research Centre, Bangladesh

    Google Scholar 

  40. Roberts DP, Denny TP, Schell MA (1988) Cloning of the egl gene of Pseudomonas solanacearum and analysis of its role in phytopathogenicity. J Bacteriol 170:1445–1451

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Salimath PM, Bahl PN (1985) Heterosis and combining ability for earliness in chickpea (Cicer arietinum L.). Indian J Genet 45:97–100

    Google Scholar 

  42. Scott JW, Somodi GC, Jones JB (1988) Bacterial spot resistance is not associated with bacterial wilt resistance in tomato. Proc Flo St Hort Soc 101:390–392

    Google Scholar 

  43. Sharma KC, Gupta JC, Rathee VK (2005) Genetics of resistance to bacterial wilt in tomato (Lycopersicon esculentum Mill.). In: Proceedings of Integrated Plant Disease management: Challenging Problems in horticultural and forest pathology, Solan, India, 14–15 November, 2003, pp. 137–141.

  44. Shende VD, Seth T, Mukherjee S, Chattopadhyay A (2012) Breeding tomato (Solanum lycopersicum L.) for higher productivity and better processing qualities. SABRAO J Breed Genet. 44(2):302–321

    Google Scholar 

  45. Singh Ramesh K, Rai N, Major S, Singh SN, Srivastava K (2014) Genetic analysis to identify good combiners for ToLCV resistance and yield components in tomato using interspecific hybridization. J Genet 93(3):623–629

    Article  CAS  PubMed  Google Scholar 

  46. Solieman TH, El-Gabry MAH, Abido A (2013) Heterosis, potence ratio and correlation of some important characters in tomato (Solanum lycopersicum L.). Sci Hort 150:25–30

    Article  Google Scholar 

  47. Thoquet P, Olivier J, Sperisen C, Rogowsky P, Laterrot H, Grimsley N (1996) Quantitative trait loci determining resistance to bacterial wilt in the tomato cultivar Hawaii 7996. Mol Plant Microbe Interact 9:826–836

    Article  CAS  Google Scholar 

  48. Thoquet P, Olivier J, Sperisen C, Rogowsky P, Prior P, Anaïs G, Mangin B, Bazin B, Nazer R, Grimsley N (1996) Polygenic resistance of tomato plants to bacterial wilt in the French West Indies. Mol Plant Microbe Interact 9:837–842

    Article  CAS  Google Scholar 

  49. Tomasik AA, Goodman RN (1986). Pathogenesis, migration and population dynamics of Erwinia amylovora in susceptible Jonathan and resistant Red Delicious apple shoots. In: EL Civerolo, A Collmer, RE Davis and AG Gillaspie. (eds.) Plant pathogenic bacteria: proceedings of the sixth international conference on plant pathogenic bacteria. Maryland.

  50. Winstead NN, Kelman A (1952) Inoculation technique for evaluating resistance to Pseudomonas solanacearum. Phytopath 42:628–634

    Google Scholar 

  51. Wynne JC, Emery DA, Rice PM (1970) Combining ability estimates in Arachis hypogaea L.II Field performance hybrids. Crop Sci 10(6):713–715

    Article  Google Scholar 

  52. Yabuuchi E, Kosako Y, Yano L, Hotta H, Nishiuchi Y (1996) Validation of the publication of new names and new combinations previously effectively published outside the IJSB. Int J Syst Bacteriol 46:625–626

    Article  Google Scholar 

  53. Yadeta KA, Thomma BPHJ (2013) The xylem as battleground for plant hosts and vascular wilt pathogens. Front Plant Sci 4:97

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

Authors wish to acknowledge the help and cooperation rendered by the Project Coordinator, AICRP on Vegetable Crops, IIVR, Varanasi, India, and the Officer-in-Charge, AICRP on Vegetable Crops, OUAT, Bhubaneswar, Odisha, India, for providing partial financial support and bacterial wilt tolerant varieties to conduct the entire study.

Author information

Authors and Affiliations

  1. Department of Vegetable Science, Faculty of Horticulture, Bidhan Chandra Krishi Viswavidyalaya, Nadia, Mohanpur, 741252, West Bengal, India

    Subhramalya Dutta, Arup Chattopadhyay, Brati Acharya & Pranab Hazra

  2. Department of Plant Pathology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Nadia, Mohanpur, 741252, West Bengal, India

    Subrata Dutta & Asit Kumar Mandal

Authors
  1. Subhramalya Dutta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arup Chattopadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Brati Acharya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Subrata Dutta
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Asit Kumar Mandal
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pranab Hazra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arup Chattopadhyay.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dutta, S., Chattopadhyay, A., Acharya, B. et al. Heterosis Breeding Could Improve Root and Stem Anatomical Traits Conferring Bacterial Wilt Disease Tolerance in Tomato Cultivars. Agric Res 11, 341–351 (2022). https://doi.org/10.1007/s40003-021-00571-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40003-021-00571-x

Keywords

Search

Navigation