Micromorphological and Anatomical Evaluation of In Vitro and Field Transferred Plants of Coccinia indica
- 38 Downloads
The micromorphological and anatomical changes in micropropagated plantlets of Coccinia indica grown under field conditions were studied. In vitro regeneration of plantlets under heterotrophic conditions is responsible for the induction of plant’s physiological and structural modifications. Significant developmental changes were observed from in vitro to the in vivo shifted plantlets. The micromorphological alterations in terms of stomatal index (from 23.0 to 19.2), vein islets (from 6.2 to 11.5), trichomes (from 11.0 to 16.0), epidermal cells and mesophyll tissues revealed the effective metabolism of photosynthesis and respiration from in vitro to field environment. Abnormal hypertrophied cortical cells with large intercellular spaces and retarded vascular cells were reported from the in vitro grown stem and root, whereas compact storage cells and advanced vascular tissues with the development of secondary xylem and phloem tissues were observed in field transferred plants. Field transfer is stressful to the micropropagated plantlets, which could be repaired by maintaining in vitro regenerated plantlets in the greenhouse to induce the plasticity in the plantlets. This study could help in understanding the abnormal tissues organization and functions of stomata behind the poor acclimatization process which leads to the poor survival of micropropagated plantlets in the field.
KeywordsAcclimatization Coccinia indica Anatomy Morphology Micropropagation
Formaldehyde acetic acid ethyl alcohol
Indole-3 butyric acid
Murashige and Skoog’s medium
Authors are grateful to the Department of Science, Technology and Environment, Government of Puducherry, India, for providing financial support to their laboratory as Grant–In-Aid Scheme.
MM designed and performed the experiments. MSS interpreted the results and wrote the manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest in this publication.
- 7.Deokate UA, Khadabadi SS (2012) Pharmacology and phytochemistry of Coccinia indica. Pharmacophore 3:179–185Google Scholar
- 8.El-Zaher MHA (2008) Studies on micropropagation of Jackfruit 1- behavior of the jackfruit plants through the micropropagation stages. World J Agric Sci 4:263–279Google Scholar
- 9.Facciola S (1990) Cornucopia: a source book of edible plants. Kampong Publications, VistaGoogle Scholar
- 10.Gradist P, Purintrapiban J (2009) The biological activity of Coccinia indica on glucose transporter 1(GLUT1) promoter. Songklanakarni J Sci Technol 31:247–253Google Scholar
- 11.Hazarika BN (2003) Acclimatization of tissue-cultured plants. Curr Sci 85:1704–1712Google Scholar
- 14.Johansen DA (1940) Plant microtechnique. McGraw Hill Book Co., New York, pp 182–197Google Scholar
- 15.Kubota C (2002) Photoautotrophic micropropagation: importance of controlled environment in plant tissue culture. Comb Proc Int Plant Propag Soc 52:609–613Google Scholar
- 18.Mathur S, Shekhawat GS, Batra A (2008) Somatic embryogenesis from cotyledon explants of Salvodora persica L. Phytomorphol 58:57–63Google Scholar
- 21.Paunescu A (2008) In vitro and in vivo variability of histological traits of Dianthus callizonus (Caryophyllaceae) aerial vegetative organs. Phytol Balc 144:17–423Google Scholar
- 22.Pospisilova J, Catsky J, Sestak Z (1997) Photosynthesis in plants cultivated in vitro. In: Pessarakli M (ed) Handbook of photosynthesis. Marcel Dekker Inc, New YorkGoogle Scholar
- 25.Prabhakar M (2004) Structure, delimitation, nomenclature and classification of stomata. Acta Bot Sin 46:242–252Google Scholar
- 26.Preece JE, Sutter EG (1991) Acclimatization of micropropagation plants to the greenhouse and field. In: Debergh PC, Zimmerman RD (eds) Micropropagation technology and application. Kluwer Academic Publishers, Dordrecht, pp 77–93Google Scholar
- 29.Sarker PFMS, Jahan R, Rahmatullah M (2009) In vitro regeneration of Coccinia grandis (L.) Voigt. an indigenous medicinal plant of Bangladesh. Afr J Tradit Complement Altern Med (Abst). World Congress on Medicinal and Aromatic Plants, Cape Town, South AfricaGoogle Scholar
- 30.Sass JE (1940) Elements of botanical microtechnique. McFraw-Hill Book Co, New York, p 222Google Scholar
- 33.Shekhawat MS, Ravindran CP, Manokari M (2014) Development of hormonal regulation of direct shoots and roots regeneration in Coccinia indica L. Int J Nat Sci Res 2:103–112Google Scholar
- 36.Taiz L, Zeiger E (2006) Plant physiology. Sinauer Associates Inc, SunderlandGoogle Scholar
- 37.Taware AS, Mukadam DS, Chavan AM, Taware SD (2010) Comparative studies of in vitro and in vivo grown plants and callus of Stevia rebaudiana (Bertoni). Int J Integr Biol 9:10–15Google Scholar
- 38.Thiripurasundari U, Rao MV (2012) Indirect organogenesis from nodal explants of Coccinia grandis (L.) Voigt. Indian J Biotechnol 11:352–354Google Scholar
- 40.Yadav G, Mishra A (2010) Medical properties of Ivy gourd (Cephalandra indica): a review. Int J Pharma Res Dev 2:92–98Google Scholar