Skip to main content
SpringerLink
Log in

Efficiency of Phosphatases in Mobilization of Native Phosphorus Fractions Under Different Vegetation

  • Full-Length Research Article
  • Published:
Agricultural Research Aims and scope Submit manuscript

Abstract

The efficiency of acid and alkaline phosphatase toward hydrolysis of different P fractions was studied under fallow, rhizosphere and non-rhizosphere soils of crops, trees and grasses from Aridisol, and forest rhizosphere and non-rhizosphere soils of Inceptisol. Native P of soil was fractioned into four different pools: water-soluble inorganic phosphorus (Pi) and organic phosphorus (Po), NaHCO3 (0.5 M)-extractable Pi and Po, NaOH (0.1 M)-extractable Pi and Po, HCl (1.0 M)-extractable Pi and Po and residual P after digestion to H2O2:H2SO4 (1:4). The decrease in different Po fractions due to the action of both acid and alkaline phosphatase showed 41.3–86.7 % of water-soluble fraction, 50–84.1 % of NaHCO3 fraction, 14.6–25.9 % of NaOH fraction and 8.0–19.2 % of HC1 fraction was hydrolyzed under different vegetation. The rate of organic phosphorus hydrolysis by acid and alkaline phosphatase was initially rapid followed by a gradual declining rate, which was constant after 8 h onwards for acid phosphatase and after 9 h onward till 24 h for alkaline phosphatase. At initial stage (<4 h), the rate of hydrolysis by acid phosphatase was almost doubled than alkaline phosphatase. The result indicates that acid phosphatase was 8.8–13.8 % more efficient as compared to the alkaline phosphatase toward hydrolysis of different soil P fractions, while the residual P fractions were hydrolyzed 17.3 % more by alkaline phosphatase than acid phosphatase. Among the different P fractions, the residual P was most resistant and water soluble and NaHCO3-P was most susceptible to phosphatase enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Anderson G (1980) Assessing organic phosphorus in soils. In: Khasawneh FE, Sample EC, Kamprath EJ (eds) The role of phosphorus in agriculture. American Society of Agronomy, Madison, pp 411–432

    Google Scholar 

  2. Bowman RA, Cole CV (1978) An exploratory method for fractionation of organic phosphorus from grassland soil. Soil Sci 125:95–101

    Article  CAS  Google Scholar 

  3. Cade- Menum BJ, Berch SM, Preston CM, Lavkulich LM (2000) Phosphorus forms and related soil chemistry of podzolic soils on northern Vancouver Island. I. A comparison of two forest types. Can J For Res 30:1714–1725

    Article  Google Scholar 

  4. Chen CR, Condron LM, Sinaj S, Davis MR, Sherlock RR, Frossard E (2003) Effect of plant species on phosphorus availability in a range of grassland soils. Plant Soil 256:115–130

    Article  CAS  Google Scholar 

  5. Dinkelaker B, Marschner H (1992) In vivo demonstration of acid phosphatase activity in the rhizosphere of soil grown plants. Plant Soil 144:199–205

    Article  CAS  Google Scholar 

  6. Dong D, Peng X, Yan X (2004) Organic acid exudation induced by phosphorus deficiency and/or aluminum toxicity in two contrasting soybean genotypes. Physiol Plant 122:190–199

    Article  CAS  Google Scholar 

  7. Fixen PE, Grove JH (1990) Testing soils for phosphorus. In: Westerman RL (ed) Soil testing and plant analysis. Soil Science Society of America (SSSA), Madison, pp 141–180

    Google Scholar 

  8. Föhse D, Claassen N, Jungk A (1988) Phophorus efficiency of plants I. External and internal P requirement and P uptake efficiency of different plant species. Plant Soil 110:101–109

    Article  Google Scholar 

  9. Föhse D, Claassen N, Jungk A (1991) Phosphorus efficiency of plants. II. Significance of root radius, root hairs and cation anion balance for phosphorus influx in seven plant species. Plant Soil 132:261–272

    Google Scholar 

  10. Geoge TS, Gregory PJ, Wood M, Read D, Bresh RJ (2002) Phosphatase activity and organic acids in the rhizosphere of potential agroforestry species and maize. Soil Biol Biochem 34:1487–1497

    Article  Google Scholar 

  11. Grierson PF, Adams MA (2000) Plant species affect acid phosphatase, ergosterol and microbial P in a jarrah (Eucalyptus marginata Donn ex Sm.) forest in south-western. Soil Biol Biochem 32:1817–1827

    Article  CAS  Google Scholar 

  12. Haussling M, Marschner H (1989) Organic and inorganic soil phosphates and acid phosphatase activity in the rhizosphere of 80-year-old Norway spruce (Picea abies (L.) Karst.) trees. Biol Fertil Soils 8:128–133

    Article  Google Scholar 

  13. Hayes JE, Richardson AE, Simpson RJ (2000) Components of organic phosphorus in soil extracts that are hydrolyzed by phytase and acid phosphatase. Biol Fertil Soils 32:279–286

    Article  CAS  Google Scholar 

  14. Haynes RJ, Swift RS (1988) Effects of liming and phosphate addition on changes in enzyme activities, microbial biomass and levels of extractable nitrogen, sulfur, and phosphorus in an acid soil. Biol Fertil Soils 6:153–158

    Article  CAS  Google Scholar 

  15. Jackson ML (1967) Soil chemical analysis. Prentice-Hall of India, Delhi, p 498

    Google Scholar 

  16. Jones DL, Dennis PG, Owen AG, Van Hees PAW (2003) Organic acid behaviour in soils—misconceptions and knowledge gaps. Plant Soil 248:31–41

    Article  CAS  Google Scholar 

  17. Kucey RMN, Janzen HH, Leggett ME (1989) Microbially mediated increases in plant-available phosphorus. Adv Agron 42:199–228

    Article  CAS  Google Scholar 

  18. McCallister DL, Bahadir MA, Blumenthal JM (2002) Phosphorus partitioning and phosphatase activity in semi-arid region soils under increasing crop growth intensity. Soil Sci 167:616–624

    Article  CAS  Google Scholar 

  19. Mehta NC, Legg JO, Goring CAI, Black CA (1954) Determination of organic phosphorus in soil. I. Extraction method. Soil Sci Soc Am Proc 18:443–449

    Article  CAS  Google Scholar 

  20. Oberson A, Fardeau JC, Besson JM, Sticher H (1993) Soil phosphorus dynamics in cropping systems managed according to conventional and biological agricultural methods. Biol Fertil Soils 16:111–117

    Article  CAS  Google Scholar 

  21. Oberson A, Besson JM, Maire N, Sticher H (1996) Microbiological processes in soil organic phosphorus transformations in conventional and biological cropping systems. Biol Fertil Soils 21:138–148

    Article  CAS  Google Scholar 

  22. Oberson A, Friesen DK, Tiessen H, Morel C, Stahel W (1999) Phosphorus status and cycling in native savanna and improved pastures on an acid low-P Colombian Oxisol. Nutr Cycl Agroecosyst 55:77–88

    Article  Google Scholar 

  23. Pant HK, Edwards AC, Vaughan D (1994) Extraction, molecular fractionation and enzyme degradation of organically associated phosphorus in soil solutions. Biol Fertil Soils 17:196–200

    Article  CAS  Google Scholar 

  24. Radersma S, Grierson PF (2004) Phosphorus mobilization in agroforestry: organic anions, phosphatase activity and phosphorus fractions in the rhizosphere. Plant Soil 259:209–219

    Article  CAS  Google Scholar 

  25. Römer W, Beissner L, Schenk H, Jungk A (1995) Einflussvon sorte und phosphordungung auf den phosphorgehalt und die Aktivitat der sauren phosphatases von Weizen und Gersle. Ein Beitrag Zur Diagnose der P-versorgung von Pflanzon. Zeitschrift Pflanzenernähr Bodenkunde 158:3–8

    Article  Google Scholar 

  26. Seeling B, Jungk A (1996) Utilization of organic phosphorus in calcium chloride extracts of soil by barley plants and hydrolysis of acid and alkaline phosphatases. Plant Soil 178:179–184

    Article  CAS  Google Scholar 

  27. Shand CA, Macklon AES, Edwards AC, Smith S (1994) Inorganic and organic P in soil solutions from three upland soils. I. Effect of soil solution extraction conditions, soil type and season. Plant Soil 159:255–264

    Article  CAS  Google Scholar 

  28. Tabatabai MA (1982) Soil enzymes. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, part 2. American Society of Agronomy, Madison, pp 903–947

    Google Scholar 

  29. Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307

    Article  CAS  Google Scholar 

  30. Tarafdar JC (1998) Hydrolysis of organic P by acid phosphatases in a cambisol. J Ind Soc Soil Sci 46:310–313

    CAS  Google Scholar 

  31. Tarafdar JC, Claassen N (1988) Organic phosphorus compounds as a phosphorus source for higher plants through the activity of phosphatases produced by plant roots and microorganisms. Biol Fertil Soils 5:308–312

    Article  CAS  Google Scholar 

  32. Tarafdar JC, Jungk A (1987) Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol Fertil Soils 3:199–204

    Article  CAS  Google Scholar 

  33. Tarafdar JC, Marschner H (1994) Efficiency of VAM hyphae in utilization of organic phosphorus by wheat plants. Soil Sci Plant Nutr 40:593–600

    Article  CAS  Google Scholar 

  34. Tarafdar JC, Yadav RS, Niwas R (2002) Relative efficiency of fungal intra and extracellular phosphatases and phytase. J Plant Nutr Soil Sci 165:17–19

    Article  CAS  Google Scholar 

  35. Tarafdar JC, Yadav RS, Bareja M, Singh G (2006) Phosphorus fractionation under crops, trees and grasses. J Ind Soc Soil Sci 54:38–44

    Google Scholar 

  36. Turner BL, McKelvie ID, Haygarth PM (2002) Characterisation of water-extractable soil organic phosphorus by phosphatase hydrolysis. Soil Biol Biochem 34:27–35

    Article  CAS  Google Scholar 

  37. Turner BL, Cade-Menun BJ, Westermann DT (2003) Organic phosphorus composition and potential bioavailability in semi-arid arable soils of the Western United States. Soil Sci Soc Am J 67:1168–1179

    Article  CAS  Google Scholar 

  38. Yadav RS, Tarafdar JC (2003) Phytase and phosphatases producing fungi in arid and semi-arid soils and their efficiency in hydrolyzing different organic P. Soil Biol Biochem 35:745–751

    Article  CAS  Google Scholar 

  39. Zhang BG, Li GT (1998) Roles of soil organisms on the enhancement of plant availability of soil phosphorus. Acta Pedol Sin 35:104–111

    CAS  Google Scholar 

  40. Zhou YY, Li JQ, Zhang M (2001) Vertical variations in kinetics of alkaline phosphatase and P species in sediments of a shallow Chinese eutrophic lake (Lake Donghu). Hydrobiologia 450:91–98

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Jai Narain Vyas University, Jodhpur, Rajasthan, 342005, India

    Amita Dhariwal Gharu

  2. ICAR-Central Arid Zone Research Institute, Jodhpur, Rajasthan, 342003, India

    J. C. Tarafdar

Authors
  1. Amita Dhariwal Gharu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. C. Tarafdar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. C. Tarafdar.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gharu, A.D., Tarafdar, J.C. Efficiency of Phosphatases in Mobilization of Native Phosphorus Fractions Under Different Vegetation. Agric Res 5, 335–345 (2016). https://doi.org/10.1007/s40003-016-0235-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40003-016-0235-5

Keywords

Search

Navigation